Gelation time, homogeneity, and rupture testing of alginate‐calcium carbonate‐hydrogen peroxide gels for use as wound dressings

Alexander, Brendan; Murphy, Kathleen E.; Gallagher, Joanne; Farrell, Garrett F.; Taggart, Gertie

doi:10.1002/jbm.b.31965

Cited by 13 publications

(8 citation statements)

References 21 publications

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“…Figure 2 displays the gel homogeneity, estimated according to the literature [38]. The results show that, despite shrinking, the coating does not substantially influence the homogeneity of the gels.…”

Section: Resultsmentioning

confidence: 99%

“…Gelation time, defined as the time between the addition of GDL and the formation of a fixed gel, was assessed using a method adapted from the literature [38]. The petri dish containing the gelling solution was periodically tilted during the gelation process.…”

Section: Methodsmentioning

confidence: 99%

“…The homogeneity of alginate gels was estimated according to the literature [38]. Hydrogels were cut into four slices of equal dimension (a–d).…”

Section: Methodsmentioning

confidence: 99%

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Alginate Hydrogels Coated with Chitosan for Wound Dressing

Straccia

d’Ayala

Romano³

et al. 2015

Marine Drugs

143

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In this work, a coating of chitosan onto alginate hydrogels was realized using the water-soluble hydrochloride form of chitosan (CH-Cl), with the dual purpose of imparting antibacterial activity and delaying the release of hydrophilic molecules from the alginate matrix. Alginate hydrogels with different calcium contents were prepared by the internal setting method and coated by immersion in a CH-Cl solution. Structural analysis by cryo-scanning electron microscopy was carried out to highlight morphological alterations due to the coating layer. Tests in vitro with human mesenchymal stromal cells (MSC) were assessed to check the absence of toxicity of CH-Cl. Swelling, stability in physiological solution and release characteristics using rhodamine B as the hydrophilic model drug were compared to those of relative uncoated hydrogels. Finally, antibacterial activity against Escherichia coli was tested. Results show that alginate hydrogels coated with chitosan hydrochloride described here can be proposed as a novel medicated dressing by associating intrinsic antimicrobial activity with improved sustained release characteristics.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Alginate Hydrogels Coated with Chitosan for Wound Dressing

Straccia

d’Ayala

Romano³

et al. 2015

Marine Drugs

143

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“…Ca 2+ ions diffuse from the continuous phase into alginate droplets cross-linking them and forming gel particles [21]. The main disadvantage of this method is the formation of non-uniform alginate hydrogels due to the establishment of a gradient of Ca 2+ concentration towards the boundary of the hydrogel where it is in contact with the solution of Ca 2+ and an extremely high rate of the cross-linking reaction [30,31,32]. To some extent, the problem of non-uniform distribution of the cations in external gelation method can be eliminated using alginates of higher molecular weights or carrying out gelation in the buffer solutions containing phosphate ions that also bind calcium ions and, in this way, compete with alginate.…”

Section: Ca2+-alginate Gel Based Scaffoldsmentioning

confidence: 99%

Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

2019

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Formulation of multifunctional biopolymer-based scaffolds is one of the major focuses in modern tissue engineering and regenerative medicine. Besides proper mechanical/chemical properties, an ideal scaffold should: (i) possess a well-tuned porous internal structure for cell seeding/growth and (ii) host bioactive molecules to be protected against biodegradation and presented to cells when required. Alginate hydrogels were extensively developed to serve as scaffolds, and recent advances in the hydrogel formulation demonstrate their applicability as “ideal” soft scaffolds. This review focuses on advanced porous alginate scaffolds (PAS) fabricated using hard templating on vaterite CaCO3 crystals. These novel tailor-made soft structures can be prepared at physiologically relevant conditions offering a high level of control over their internal structure and high performance for loading/release of bioactive macromolecules. The novel approach to assemble PAS is compared with traditional methods used for fabrication of porous alginate hydrogels. Finally, future perspectives and applications of PAS for advanced cell culture, tissue engineering, and drug testing are discussed.

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“…Ionically crosslinked polymer gels such as alginate hydrogels have been extensively developed as scaffolds for tissue engineering . The adjustable kinetics of the alginate gel degradation at neutral pH gives an option to use the gels for multiple applications as wound dressings, anti‐adhesive, and repair materials . Characteristics of alginate gels (hydration, softness, porosity, swelling in water, etc.)…”

Section: Introductionmentioning

confidence: 99%

Design of Porous Alginate Hydrogels by Sacrificial CaCO₃ Templates: Pore Formation Mechanism

Sergeeva

Feoktistova

Prokopović

et al. 2015

Adv Materials Inter

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signifi cantly precise control over the scaffold architecture. Well-defi ned internal structure (porosity) determines both cellular infi ltration into the scaffold and its material properties. Pore size and distribution ensuring diffusion of nutrients into the scaffold and removal of metabolic products is of high importance. [ 4,11 ] Ionically crosslinked polymer gels such as alginate hydrogels have been extensively developed as scaffolds for tissue engineering. [12][13][14][15] The adjustable kinetics of the alginate gel degradation at neutral pH [ 1,16,17 ] gives an option to use the gels for multiple applications as wound dressings, anti-adhesive, and repair materials. [ 14,[18][19][20][21] Characteristics of alginate gels (hydration, softness, porosity, swelling in water, etc.) can be adjusted using a certain fabrication approach and by variation of interactions between charged polyanionic groups and crosslinking counterions; [ 13,17,[22][23][24][25] these interactions are known to be strongly affected by ionic strength, molecule length and structure, solvent pH, salt concentration, and temperature conditions. [26][27][28][29][30][31] This allows the fabrication of alginate scaffolds possessing desired properties corresponding to a certain tissue, for instance cartilage, [ 12 ] or dura mater. [ 21 ] Porous alginate gels can provide space and mechanical support to seed biological cells for tissue formation, [ 1,13,32 ] also allowing a controlled release of gel-laden drugs (peptides and proteins) trapped within the alginate network. [ 14,24,25,33,34 ] To achieve loading of both biological cells and bioactive molecules, the internal structure of hydrogels has to be controlled on the scale of nano-and micrometers. Adjustment of the gel geometry has been demonstrated on substrate surfaces using different micropatterning techniques including a light-addressable electrolytic system, [ 14 ] electrodeposition, [ 15 ] electrochemical patterning, [ 35 ] or the benchtop method using Nylon mesh. [ 11 ] The internal structure of alginate gels has been patterned with regular tube-like pores, [ 16,36 ] interconnected ordered honeycomb pores, [ 12 ] and sponge-like isotropic pores. [37][38][39] However, most of the approaches for alginate gel fabrication lack a precise control over the microstructure and require special equipment. Encapsulation of molecules of interest with controlled spatial distribution is rather complicated or even impossible. There is a need to develop a simple approach for design of a scaffold with welldefi ned internal structure providing both a space to culture cells and cavities to host/release encapsulated (bio)active molecules.Fabrication of porous alginate hydrogels with a well-controlled architecture useful for tissue engineering is still a challenge. Here, CaCO 3 -based templating is utilized to design stable alginate gels with controlled pore dimensions in the range of 5-50 µm. The mechanism of pore formation is studied considering two factors affecting the pore size: i) osmotic pressure generat...

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Gelation time, homogeneity, and rupture testing of alginate‐calcium carbonate‐hydrogen peroxide gels for use as wound dressings

Cited by 13 publications

References 21 publications

Alginate Hydrogels Coated with Chitosan for Wound Dressing

Alginate Hydrogels Coated with Chitosan for Wound Dressing

Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

Design of Porous Alginate Hydrogels by Sacrificial CaCO₃ Templates: Pore Formation Mechanism

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Gelation time, homogeneity, and rupture testing of alginate‐calcium carbonate‐hydrogen peroxide gels for use as wound dressings

Cited by 13 publications

References 21 publications

Alginate Hydrogels Coated with Chitosan for Wound Dressing

Alginate Hydrogels Coated with Chitosan for Wound Dressing

Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

Design of Porous Alginate Hydrogels by Sacrificial CaCO3 Templates: Pore Formation Mechanism

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Product

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Design of Porous Alginate Hydrogels by Sacrificial CaCO₃ Templates: Pore Formation Mechanism