Gellan gum-gelatin scaffolds with Ca2+ crosslinking for constructing a structured cell cultured meat model

Chen, Yan; Li, Linzi; Chen, Lin; Shao, Wei; Chen, Xiaohong; Fan, Xiaojing; Liu, Yaping; Ding, Shijie; Xu, Xinglian; Zhou, Guanghong; Feng, Xianchao

doi:10.1016/j.biomaterials.2023.122176

Cited by 26 publications

(7 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent study, Chen et al showed the faster degradation of GG−gelatin scaffolds when compared to GG alone. 32 Both GG and AG-GG hydrogels underwent gradual hydrolytic degradation, and the time frame for degradation was found to be suitable for cellular infiltration and ECM deposition for vocal fold tissue regeneration. 33 Previous studies have shown that gellan gum undergoes surface erosion, 34 but the actual degradation kinetics and mechanism will be known only through in vivo implantation, which is our focus in the future.…”

Section: ■ Discussionmentioning

confidence: 97%

Injectable Tissue Adhesive Microgel Composite Containing Antifibrotic Drug for Vocal Fold Scarring

John,

Nabizath,

Krishnakumar

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Vocal fold (VF) scarring, a complex problem in laryngology, results from injury and inflammation of the layered architecture of the VFs. The resultant voice hoarseness, for which successful therapeutic options are currently limited, affects the patient's quality of life. A promising strategy to reverse this disorder is the use of antifibrotic drugs. The present study proposes a novel microbead-embedded injectable hydrogel that can sustain the release of the antifibrotic drug pirfenidone (PFD) for vocal fold scarring. Microbeads were developed using sodium alginate and gelatin, which were further embedded into a biomimetic and tissue adhesive gellan gum (GG) hydrogel. The microbeadembedded hydrogel exhibited improved injectability, viscoelasticity, tissue adhesiveness, degradability, and swelling compared to the hydrogel without beads. Additionally, the bead-embedded hydrogel could sustain the release of the PFD for a week. In vitro studies showed that the drug-loaded hydrogel could reduce the migration and proliferation of fibroblast cells in a dose-dependent manner. In summary, this study demonstrates the potential of a PFD-loaded injectable hydrogel with enhanced viscoelastic and tissue-adhesive properties for vocal fold scarring applications.

show abstract

Section: ■ Discussionmentioning

confidence: 97%

Injectable Tissue Adhesive Microgel Composite Containing Antifibrotic Drug for Vocal Fold Scarring

John,

Nabizath,

Krishnakumar

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…Most 3D scaffolds are manufactured using natural materials, such as animal collagen, chitosan, and vegetable cellulose, as they are often consumed as-is after producing cultured meat ( Moslemy et al, 2023 ). In most cases, collagen is made into hydrogel or MC, and because it is already extracted from animal protein, this scaffold has a texture similar to meat ( Chen et al, 2023 ). However, when used as a cultured meat scaffold, if the concentration is too high or the amount is too large, hardness increases, and the textural preference is lower than that of meat ( Grønlien et al, 2019 ).…”

Section: Textural Characteristics Of Cultured Meatmentioning

confidence: 99%

The Need for Research on the Comparison of Sensory Characteristics between Cultured Meat Produced Using Scaffolds and Meat

Lee,

Choi

2024

Food Sci Anim Resour

View full text Add to dashboard Cite

Cultured meat is one of the research areas currently in the spotlight in the agricultural and livestock industry, and refers to cells obtained from livestock that are proliferated and differentiated and processed into edible meat. These cell-cultured meats are mainly studied at the lab-scale by culturing them in flasks, and for commercial use, they are produced using scaffolds that mimic cell supports. Scaffolds are broadly divided into fiber scaffolds, hydrogels, and micro-carrier beads, and these are classified according to processing methods and materials. In particular, a scaffold is essential for mass production, which allows it to have appearance, texture, and flavor characteristics similar to meat. Because cultured meat is cultured in a state where oxygen is blocked, it may be lighter in color or produce less flavor substances than edible meat, but these can be compensated for by adding natural substances to the scaffolds or improving fat adhesion. In addition, it has the advantage of being able to express the texture characteristics of the scaffolds that make up the meat in various ways depending on the materials and manufacturing methods of the scaffolds. As a result, to increase consumers’ preference for cultured meat and its similarity to edible meat, it is believed that manufacturing scaffolds taking into account the characteristics of edible meat will serve as an important factor. Therefore, continued research and interest in scaffolds is believed to be necessary.

show abstract

“…One area of interest is cultivated meat, which uses modern engineering and biotechnology to produce animal proteins and meat products, i.e., fat and muscle tissue, for human consumption. , The process of creating cultivated meat can be summarized into four stages: (1) collection of cells from an animal, (2) expansion of cells in a bioreactor, (3) distribution of cells and tissue development on a scaffold, and (4) final packing and distribution to the customer . For the development of structured meat products, the challenge is manufacturing scaffolds to accurately portray a tissue’s structural and physicomechanical properties to guide proper tissue development. − …”

Section: Introductionmentioning

confidence: 99%

Whey Protein Isolate Composites as Potential Scaffolds for Cultivated Meat

Charron,

Tahir,

Foley

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Modern food technology has given rise to numerous alternative protein sources in response to a growing human population and the negative environmental impacts of current food systems. To aid in achieving global food security, one such form of alternative protein being investigated is cultivated meat, which applies the principles of mechanical and tissue engineering to produce animal proteins and meat products from animal cells. Herein, nonmodified and methacrylated whey protein formed hydrogels with methacrylated alginate as potential tissue engineering scaffolds for cultivated meat. Whey protein is a byproduct of dairy processing and was selected because it is an approved food additive and cytocompatible and has shown efficacy in other biomaterial applications. Whey protein and alginate scaffolds were formed via visible light cross-linking in aqueous solutions under ambient conditions. The characteristics of the precursor solution and the physical−mechanical properties of the scaffolds were quantified; while gelation occurred within the homo-and copolymer hydrogels, the integrity of the network was significantly altered with varying components. Qualitatively, the scaffolds exhibited a three-dimensional (3D) interconnected porous network. Whey protein isolate (WPI)-based scaffolds were noncytotoxic and supported in vitro myoblast adhesion and proliferation. The data presented support the hypothesis that the composition of the hydrogel plays a significant role in the scaffold's performance.

show abstract

Gellan gum-gelatin scaffolds with Ca2+ crosslinking for constructing a structured cell cultured meat model

Cited by 26 publications

References 60 publications

Injectable Tissue Adhesive Microgel Composite Containing Antifibrotic Drug for Vocal Fold Scarring

Injectable Tissue Adhesive Microgel Composite Containing Antifibrotic Drug for Vocal Fold Scarring

The Need for Research on the Comparison of Sensory Characteristics between Cultured Meat Produced Using Scaffolds and Meat

Whey Protein Isolate Composites as Potential Scaffolds for Cultivated Meat

Contact Info

Product

Resources

About