Gemini surfactant-based nanoparticles T-box1 gene delivery as a novel approach to promote epithelial stem cells differentiation and dental enamel formation

Mohabatpour, Fatemeh; Al‐Dulaymi, Mays; Lobanova, Liubov; Scutchings, Brittany; Papagerakis, Silvana; Badea, Ildikó; Chen, Daniel; Papagerakis, Pétros

doi:10.1016/j.bioadv.2022.212844

Cited by 4 publications

(3 citation statements)

References 73 publications

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“…GS-based polyplexes were reported to have manyfold higher transfection efficiency than Lipofectamine2000, while also maintaining significantly higher cell viability. 452…”

Section: Cell-penetrating Peptidesmentioning

confidence: 99%

The menace of severe adverse events and deaths associated with viral gene therapy and its potential solution

Kachanov,

Kostyusheva,

Brezgin

et al. 2024

Medicinal Research Reviews

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Over the past decade, in vivo gene replacement therapy has significantly advanced, resulting in market approval of numerous therapeutics predominantly relying on adeno‐associated viral vectors (AAV). While viral vectors have undeniably addressed several critical healthcare challenges, their clinical application has unveiled a range of limitations and safety concerns. This review highlights the emerging challenges in the field of gene therapy. At first, we discuss both the role of biological barriers in viral gene therapy with a focus on AAVs, and review current landscape of in vivo human gene therapy. We delineate advantages and disadvantages of AAVs as gene delivery vehicles, mostly from the safety perspective (hepatotoxicity, cardiotoxicity, neurotoxicity, inflammatory responses etc.), and outline the mechanisms of adverse events in response to AAV. Contribution of every aspect of AAV vectors (genomic structure, capsid proteins) and host responses to injected AAV is considered and substantiated by basic, translational and clinical studies. The updated evaluation of recent AAV clinical trials and current medical experience clearly shows the risks of AAVs that sometimes overshadow the hopes for curing a hereditary disease. At last, a set of established and new molecular and nanotechnology tools and approaches are provided as potential solutions for mitigating or eliminating side effects. The increasing number of severe adverse reactions and, sadly deaths, demands decisive actions to resolve the issue of immune responses and extremely high doses of viral vectors used for gene therapy. In response to these challenges, various strategies are under development, including approaches aimed at augmenting characteristics of viral vectors and others focused on creating secure and efficacious non‐viral vectors. This comprehensive review offers an overarching perspective on the present state of gene therapy utilizing both viral and non‐viral vectors.

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“…GS-based polyplexes were reported to have manyfold higher transfection efficiency than Lipofectamine2000, while also maintaining significantly higher cell viability. 452…”

Section: Cell-penetrating Peptidesmentioning

confidence: 99%

The menace of severe adverse events and deaths associated with viral gene therapy and its potential solution

Kachanov,

Kostyusheva,

Brezgin

et al. 2024

Medicinal Research Reviews

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show abstract

“…One promising method is to employ micro/nanoparticle delivery systems that protect the incorporated GFs and modulate their release profiles [ [382] , [383] , [384] , [385] , [386] , [387] , [388] , [389] ]. The particles employed can be structurally classified as micelles, dendrimers, liposomes, solid-lipid nanoparticles, and polymeric nanoparticles [ 383 , 386 , 390 ]. The size range of particles used in controlled release varies between 20 and 1000 nm and impacts the release kinetics of the loaded bioactive molecule.…”

Section: Key Issues and Future Advances In Extrusion Bioprintingmentioning

confidence: 99%

Biomaterials / bioinks and extrusion bioprinting

Chen,

Fazel Anvari-Yazdi,

Duan

et al. 2023

Bioactive Materials

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“…Such epithelial stem cells could be used as a base for new promising approaches that would employ the three main elements of tissue regeneration i.e. stem cells, scaffolds and signaling factors with hopes to produce bioengineered enamel tissue to repair the damaged structures [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Bioprinting of alginate-carboxymethyl chitosan scaffolds for enamel tissue engineering in vitro

et al. 2022

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Three dimensional (3D) bioprinting has been emerging enabling the incorporation of living cells into the biomaterials (such a mixture is referred as a bioink) to create scaffolds. However, the bioinks available for scaffold bioprinting are limited, particularly for dental tissue engineering, due to the complicated, yet compromised, printability, mechanical and biological properties simultaneously imposed on the bioinks. This paper shows the development of a novel bioink from carboxymethyl chitosan and alginate for bioprinting scaffolds for enamel tissue regeneration. Carboxymethyl chitosan is used because to its antibacterial ability and superior cell interaction properties, while alginate was added to enhance printability and mechanical properties as well as to regulate the degradation rate. The bioinks with three mixture ratios of alginate and carboxymethyl chitosan (2-4, 3-3 and 4-2) are prepared, and then printed into the calcium chloride crosslinker solution (100 mM) to form a 3D structure of scaffolds. The printed scaffolds are characterized in terms of structural, swelling, degradation, and mechanical properties, followed by their in vitro characterization for enamel tissue regeneration. The results show that the bioinks with higher concentrations of alginate are more viscous and need higher pressure for printing; while the printed scaffolds are highly porous and show a high degree of printability and structural integrity. The hydrogels with higher carboxymethyl chitosan ratios have higher swelling ratios, faster degradation rates, and lower compressive modulus. Dental epithelial cell line, HAT-7, could maintain high viability in the printed constructs after 1, 7 and 14 days of culture. HAT-7 cells are also able to maintain their morphology and secrete alkaline phosphatase after 14 days of culture in the 3D printed scaffolds, suggesting the capacity of these cells for mineral deposition and enamel-like tissue formation. Taken together, alginate-carboxymethyl chitosan may be suitable to print scaffolds with dental epithelial cells for enamel tissue regeneration.

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Gemini surfactant-based nanoparticles T-box1 gene delivery as a novel approach to promote epithelial stem cells differentiation and dental enamel formation

Cited by 4 publications

References 73 publications

The menace of severe adverse events and deaths associated with viral gene therapy and its potential solution

The menace of severe adverse events and deaths associated with viral gene therapy and its potential solution

Biomaterials / bioinks and extrusion bioprinting

Bioprinting of alginate-carboxymethyl chitosan scaffolds for enamel tissue engineering in vitro

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