Geminiviruses: Models for Plant DNA Replication, Transcription, and Cell Cycle Regulation

Hanley‐Bowdoin, Linda; Settlage, Sharon B.; Orozco, Beverly M.; Nagar, Steven; Robertson, Dominique

doi:10.1016/s0735-2689(99)00383-4

Cited by 371 publications

(326 citation statements)

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“…Geminiviruses infect a broad variety of plants and induce a wide range of symptoms (Hanley-Bowdoin et al, 2000). Recent studies indicated that they can evade the plant immune system by interfering with host antiviral pathways Wang et al, 2003;Florentino et al, 2006;Piroux et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Geminiviruses are a group of plant DNA viruses characterized by single-stranded circular genomes encapsidated in twinned icosahedral particles that range in size from 18 to 30 nm (Hanley-Bowdoin et al, 2000;Rojas et al, 2005). They can be divided into four genera (Mastrevirus, Topocuvirus, Curtovirus, and Begomovirus) based on genome structure, insect vectors, and host range (Fauquet and Stanley, 2005).…”

mentioning

confidence: 99%

“…Within the family Geminiviridae, begomoviruses are the most numerous and geographically widespread viruses. Begomoviruses have either monopartite or bipartite genomes (Hanley-Bowdoin et al, 2000;Fauquet et al, 2003). In recent years, a b-satellite molecule, which is a circular single-stranded DNA of approximately 1,350 nucleotides, has been associated with some monopartite begomoviruses, where it is essential for the induction of typical disease symptoms (Saunders et al, 2000(Saunders et al, , 2003Briddon et al, 2001;Jose and Usha, 2003;Cui et al, 2004).…”

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confidence: 99%

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Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

et al. 2011

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The bC1 protein of tomato yellow leaf curl China b-satellite functions as a pathogenicity determinant. To better understand the molecular basis of bC1 in pathogenicity, a yeast two-hybrid screen of a tomato (Solanum lycopersicum) cDNA library was carried out using bC1 as bait. bC1 interacted with a tomato SUCROSE-NONFERMENTING1-related kinase designated as SlSnRK1. Their interaction was confirmed using a bimolecular fluorescence complementation assay in Nicotiana benthamiana cells. Plants overexpressing SnRK1 were delayed for symptom appearance and contained lower levels of viral and satellite DNA, while plants silenced for SnRK1 expression developed symptoms earlier and accumulated higher levels of viral DNA. In vitro kinase assays showed that bC1 is phosphorylated by SlSnRK1 mainly on serine at position 33 and threonine at position 78. Plants infected with bC1 mutants containing phosphorylation-mimic aspartate residues in place of serine-33 and/or threonine-78 displayed delayed and attenuated symptoms and accumulated lower levels of viral DNA, while plants infected with phosphorylation-negative alanine mutants contained higher levels of viral DNA. These results suggested that the SlSnRK1 protein attenuates geminivirus infection by interacting with and phosphorylating the bC1 protein.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

et al. 2011

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“…However, WDV-mediated RNA silencing was not impaired in the mutant lines (Fig. 3a, b), possibly because geminivirus transcription depends on host-DNA-dependent RNA polymerase (Hanley-Bowdoin et al 2000). VIGS by geminivirus might be amplified by other host RDRs such as RDR2 and RDR6 (Muangsan et al 2004).…”

Section: Discussionmentioning

confidence: 95%

Analysis of rice RNA-dependent RNA polymerase 1 (OsRDR1) in virus-mediated RNA silencing after particle bombardment

et al. 2010

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RNA-dependent RNA polymerases (RDRs) play a key role in various RNA silencing pathways in many organisms. Using the nucleotide sequence of SGS2/SDE1/ RDR6 in Arabidopsis as the search query for sequences that flank the insertions of rice retrotransposon Tos17, we selected rice mutant lines (OsRDR1). RT-PCR analysis showed that OsRDR1 mRNA was undetectable in leaves and calli of the mutants, while it was detected in wild type. RNA silencing was induced by particle bombardment to investigate any effects of OsRDR1 on RNA silencing with b-glucuronidase or green fluorescent protein DNA/RNA in the mutant lines. The results showed that RNA silencing was impaired in these mutant lines by inverted repeat (IR) DNA or in vitro transcribed double-stranded RNA. Further, the mutant lines were bombarded with Brome mosaic bromovirus (BMV, a ssRNA virus) or Wheat dwarf geminivirus (WDV, a ssDNA virus), each carrying the IR sequence of a reporter gene. As a result, RNA silencing was impaired by BMV. Interestingly, however, it was not impaired by WDV. Thus we propose that OsRDR1 is required for RNA silencing mediated by Bromovirus, but not by Geminivirus in this system.

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“…Begomoviruses are either mono or bipartite genomes each of *2.7 kb in length. DNA A encodes the coat protein and genes involved in viral replication while DNA B is involved in systemic viral movement, symptom expression and host range determination [2][3][4]. Considering the importance of weeds as reservoirs of begomoviruses, a study was undertaken to identify begomovirus species in Indian weed plants.…”

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confidence: 99%

Molecular Evidence for the Occurrence of Abutilon mosaic virus, A New World Begomovirus in India

Jyothsna

Haq

Jayaprakash³

et al. 2013

Indian J. Virol.

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During an investigation in the year 2010, on the weed reservoir of begomovirus, Abutilon pictum showing bright yellow mosaic symptoms was observed in Udhagamandalam, Tamil Nadu, India. The complete bipartite genome of a begomovirus was cloned and sequenced which revealed association of Abutilon mosaic virus (AbMV). Nicotiana benthamiana plants inoculated biolistically with the concatemers generated through rolling circle amplification of the cloned DNAs were asymptomatic; however three out of nine plants showed presence of viral DNA A. A recombination event in the ORF BC1 with ToLCNDV DNA B (HM989846) was detected. This is the first molecular evidence of AbMV in India.

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Geminiviruses: Models for Plant DNA Replication, Transcription, and Cell Cycle Regulation

Cited by 371 publications

References 0 publications

Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

Analysis of rice RNA-dependent RNA polymerase 1 (OsRDR1) in virus-mediated RNA silencing after particle bombardment

Molecular Evidence for the Occurrence of Abutilon mosaic virus, A New World Begomovirus in India

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