Geminiviruses: Molecular biodiversity and global distribution in Jatropha

More, Prashant; Agarwal, Parinita; Agarwal, Pradeep K.

doi:10.1016/j.pmpp.2019.101439

Cited by 4 publications

(2 citation statements)

References 84 publications

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“…The symptoms of the disease include mosaic and curling of leaves and malformed fruits. To date, both monopartite and bipartite geminiviruses have been detected in Jatropha from India, Kenya, Nigeria, Dominic Republican, Jamaica and the United States of America (USA) 13 .…”

Section: Introductionmentioning

confidence: 99%

Artificial miRNA mediated resistance in tobacco against Jatropha leaf curl Gujarat virus by targeting RNA silencing suppressors

Agarwal

Anand

et al. 2021

Sci Rep

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The leaf curl disease of Jatropha caused by geminiviruses results in heavy economic losses. In the present study, we report the identification of a new strain of a Jatropha leaf curl Gujarat virus (JLCuGV), which encodes six ORFs with each one having RNA silencing suppressor activity. Therefore, three artificial microRNAs (amiRNAs; C1/C4, C2/C3 and V1/V2) were designed employing overlapping regions, each targeting two ORFs of JLCuGV genomic DNA and transformed in tobacco. The C1/C4 and C2/C3 amiRNA transgenics were resistant while V1/V2 amiRNA transgenics were tolerant against JLCuGV. The relative level of amiRNA inversely related to viral load indicating a correlation with disease resistance. The assessment of photosynthetic parameters suggests that the transgenics perform significantly better in response to JLCuGV infiltration as compared to wild type (WT). The metabolite contents were not altered remarkably in amiRNA transgenics, but sugar metabolism and tricarboxylic acid (TCA) cycle showed noticeable changes in WT on virus infiltration. The overall higher methylation and demethylation observed in amiRNA transgenics correlated with decreased JLCuGV accumulation. This study demonstrates that amiRNA transgenics showed enhanced resistance to JLCuGV while efficiently maintaining normalcy in their photosynthesis and metabolic pathways as well as homeostasis in the methylation patterns.

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Section: Introductionmentioning

confidence: 99%

Artificial miRNA mediated resistance in tobacco against Jatropha leaf curl Gujarat virus by targeting RNA silencing suppressors

Agarwal

Anand

et al. 2021

Sci Rep

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“…Recently, Jatropha biofuel was for the rst time used in aircraft (Spicejet's Bombardier Q400) [3], indicating its high e ciency as fuel. The major problem in Jatropha biodiesel production is its lower yield because of various diseases like collar rot, leaf spots, powdery mildew, seedling blight, seedling stem rot and viral infestation [4]. The leaf curl mosaic disease caused by geminivirus belonging to Begomovirus genus and is a severe threat to Jatropha, causing up to 80% yield losses [5].…”

Section: Introductionmentioning

confidence: 99%

The Jatropha leaf curl Gujarat virus on infection regulates the sugar and tricarboxylic acid cycle metabolic pathways in Jatropha

Agarwal

2021

Preprint

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Jatropha, a popular biodiesel crop, suffers severe losses due to Jatropha leaf curl Gujarat virus (JLCuGV) infection in Gujarat (India). Gas chromatography-mass spectrometry (GC-MS) analysis of healthy and infected tissue revealed that the metabolites like sugars, polyols, carboxylic acids, fatty acids, polyphenols, and amino acids were regulated on JLCuGV infection. The sugars (glucose, sucrose, and fructose) increased, while carboxylic acids (malic acid, citric acid and quinic acid) and polyols (galactinol, butanetriol, triethylene glycol, myo-inositol, erythritol) decreased remarkably in infected Jatropha tissue. The increased accumulation of polyphenols in JLCuGV infected tissue suggests activation and involvement of polyphenol biosynthesis pathways as a defence response to geminivirus. All these metabolic variations indicated that sugar metabolism and tricarboxylic acid (TCA) cycle pathways are regulated as a defence response as well as a disease development response to geminivirus infection in Jatropha.

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The Jatropha leaf curl Gujarat virus on infection in Jatropha regulates the sugar and tricarboxylic acid cycle metabolic pathways

Agarwal

2022

3 Biotech

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Geminiviruses: Molecular biodiversity and global distribution in Jatropha

Cited by 4 publications

References 84 publications

Artificial miRNA mediated resistance in tobacco against Jatropha leaf curl Gujarat virus by targeting RNA silencing suppressors

Artificial miRNA mediated resistance in tobacco against Jatropha leaf curl Gujarat virus by targeting RNA silencing suppressors

The Jatropha leaf curl Gujarat virus on infection regulates the sugar and tricarboxylic acid cycle metabolic pathways in Jatropha

The Jatropha leaf curl Gujarat virus on infection in Jatropha regulates the sugar and tricarboxylic acid cycle metabolic pathways

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