Existing research about the relationship between gender diversity and performance in cooperatives is not conclusive. In view of this reality, this paper aims firstly to analyse the effectiveness of gender diversity and equality management systems (GDMS) in promoting gender diversity in the decision-making bodies (GDDB) as well as in the performance of agri-food cooperatives. Secondly, the objective is to establish the relationship between GDDB and performance, and subsequently, to analyse the moderating effect of GDDB on three of the business strategies adopted by cooperatives in order to achieve competitive advantages: Integration strategies, internationalisation strategies, and environmental concern, based on a survey carried out in 2018 using a sample of 2489 Spanish agri-food cooperatives. A moderation analysis was conducted to test the proposed model and hypotheses. The results obtained confirm a positive relationship between the implementation by cooperatives of GDMS and GDDB. The relationship between GDMS, GDDB, and performance was not significant. The moderating effect of GDDB was only statistically significant in the case of export intensity (EI) and environmental concern (EC), although, contrary to expectations, this effect was negative, meaning that the impact of both strategies on performance becomes more apparent as GDDB is reduced.