Very little research has considered how media discrimination could impact men and women’s political ambition. Yet, media discrimination could impact both beliefs about gender roles and political competence, and beliefs about voter bias, both of which could decrease women’s political ambition and increase men’s. Alternatively, media discrimination could lead women to react against discrimination and be motivated politically. This study tests how political ambition of men and women is impacted by media discrimination in a campaign and election lab experiment. Media discrimination in this experiment under-reports on women and uses traditional, stereotypical depictions of men and women. The results suggest that in certain conditions, media discrimination in political news may lead to a reactance or positive challenge effect for women, increasing their political ambition. Men, instead, may feel an aversion to entering politics, lowering their political ambition.