Gender Preselection in Cattle with Intracytoplasmically Injected, Flow Cytometrically Sorted Sperm Heads1

Hamano, Koh‐ichi; Li, Xihe; Qian, Xiaohong; Funauchi, Katsutoshi; Furudate, Makoto; Minato, Yoshiaki

doi:10.1095/biolreprod60.5.1194

Cited by 87 publications

(56 citation statements)

References 19 publications

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“…The ICSI procedure has resulted in birth of live offspring in many species [5]. Since the first attempt of bovine ICSI [6], the developmental potential of the ICSI 45 oocytes into blastocysts in vitro or live calves in vivo still low, unstable and far from satisfactory regardless of the numerous efforts [7][8][9][10][11][12][13][14][15][16]. Beside the technical difficulties caused by darkness of the ooplasm, large size of the sperm heads, and elasticity and toughness of the oolemma, the inability of the mechanical stimulation and/or the injected spermatozoon itself to induce proper oocyte activation after ICSI [17] may be responsible for this low blastocyst yields.…”

Section: Introduction 40mentioning

confidence: 99%

“…This hypothesis is supported by the low blastocyst yields after ICSI without any exogenous activation stimuli [8,10,11,[13][14][15][16]. To improve 55 the blastocyst yields after bovine ICSI, additional exogenous activation stimuli that induce intracellular calcium spike such as direct current [19], calcium ionophore [20], ionomycin [8,13,14,16,17] or ethanol [9][10][11]15,16] have been applied. However, the monotonic calcium increase triggered by these stimuli [21,22] was insufficient to completely inactivate the MPF due to re-accumulation of the cyclin B [23] and brought the oocytes to arrest again at the M-III stage 60 [17,23,24].…”

Section: Introduction 40mentioning

confidence: 99%

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A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

et al. 2009

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Regardless of the presence of sperm-borne oocyte-activating factors, activation of bovine oocytes with exogenous activation stimuli is required for further development after intracytoplasmic sperm injection (ICSI). The present study was designed to develop a new activation regimen for improving the blastocyst yield after ICSI of bovine oocytes harvested from ovaries stored at 10-12 oC for 24 h. Following ICSI, oocytes were treated with 5 μM ionomycin for 5 min, 7% ethanol for 5 or 10 min, ionomycin followed by ethanol (5 or 10 min), ionomycin followed by 10 µg/mL Cycloheximide for 5 h, or ionomycin followed by 1.9 mM 6-dimethylaminopurine for 3 h. Across the activation regimens, the cleavage rates of ICSI oocytes (45-77%) were higher than those of parthenogenetically activated oocytes (11-21%; P<0.05). Activating the ICSI oocytes with ionomycin plus ethanol improved the blastocyst yield (29-30%) comparing to the non-treated oocytes (12%; P<0.05) but the other regimens did not (9-18%; P>0.05). The higher blastocyst yields were due to increasing the proportion of ICSI oocytes that passed through the early postfertilization events until cleavage. None of the regimens have any adverse effect on the quality of the blastocysts regarding the total cell number or the proportion of the inner cell mass cells. Thus, a new activation regimen composed from two triggers for single calcium increase has been proven effective to improve the blastocyst yield after bovine ICSI using oocytes harvested from stored ovaries.Dear Dr. John P. Kastelic, Thank you very much for prompt reply and accepting our manuscript for publication in Theriogenology. We have replied for each comment from Co-editor and the reviewer-2 in pointby-point basis.Sincerely yours, Shinichi Hochi (Corresponding author)Hany Abdalla (First author)Co-editor 1-P value has been added to the Abstract (Lines 27,29).2-The number of the references in Introduction section has been reduced. Reviewer #21-Regarding omitting the two groups (5 min ethanol group and ionomycin followed by 5 min ethanol group); We do not think that the presence of these two groups causes any difficulty to recognize the superiority of ionomycin and ethanol combination. Since activation of 7% ethanol for 5 min 4 hr after ICSI is a commonly applied method for bovine ICSI, presence of such group must be important to declare the superiority of the new combination (ionomycin and ethanol) over the methods of ethanol alone under the same experimental circumstance regarding oocyte quality, injection skill, culture condition, and so on. For the ionomycin followed by 5 min ethanol, the efficiency of this method to improve the cleavage and the blastocyst yield was similar to that of ionomycin followed by 10 min ethanol, making it possible to choose the method in which oocytes are exposed to minimum exogenous stimuli. Tables 1 and 2. 2-The time of 2 nd polar body detection has been added in footnote of 15Running head: Bovine oocyte activation after ICSI * Manuscript 2 AbstractRegardless of the pres...

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Section: Introduction 40mentioning

confidence: 99%

Section: Introduction 40mentioning

confidence: 99%

A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

et al. 2009

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“…Up to ten 'ICSI-cuts' can be obtained from a single straw that can be thawed separately for its use in differed ICSI procedures (Rader et al 2016). Additionally, some authors maximize the use of valuable straws not only by using this strategy, but also by diluting and refreezing sperm doses, for example, when a frozen semen store is limited or when expensive sex-sorted sperm straws are employed (Hamano et al 1999, Rader et al 2016, Canel et al 2017.…”

Section: The Technique Step By Stepmentioning

confidence: 99%

Intracytoplasmic sperm injection in domestic and wild mammals

Salamone¹,

Canel²,

Rodríguez³

2017

Reproduction

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Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical applications in the horse-breeding industry. However, both ICSI blastocyst and offspring production continues to be limited for most farm and wild species. This article reviews technical differences of ICSI performance among species, possible biological and methodological reasons for the variable efficiency and potential strategies to improve the outcomes. One of the major applications of ICSI in animal production is the reproduction of high-value specimens. Unfortunately, some domestic species like the bovine show low rates of pronuclei formation after sperm injection, which led to the development of various artificial activation protocols and sperm pre-treatments that are discussed in this article. The impact of ICSI technique on equine breeding programs is considered in detail, since in contrast to other species, its use for elite horse reproduction has increased in recent years. ICSI has also been used to produce genetically modified animals; however, despite numerous attempts in several domestic species, only transgenic pigs have been consistently produced. Finally, the ICSI is a promising tool for genetic rescue of endangered and wild species. In conclusion, while ICSI has become a consistent ART for some species, it needs further development for others. The low results obtained for some domestic species, the high training needed and the equipment required have limited this technique to the production of elite specimens or for research purposes.Reproduction (2017) 154 F111-F124

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“…However, ICSI oocytes obviously increased their chromosomal risks at the first mitotic division compared with oocytes derived from in vitro fertilization [4]. Sexsorted [5], freeze-dried [6,7] and xenogenetic [8] spermatozoa have also been used for ICSI. Therefore, it is necessary to investigate the normality of spermatozoa for ICSI at DNA and chromosome levels.…”

Section: Introductionmentioning

confidence: 99%

A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes

Watanabe

Suzuki

Tateno

et al. 2010

J Assist Reprod Genet

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Purpose The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa. Methods Oocytes were injected with bull, ram, boar and dog sperm heads, and then fused with enucleated mouse oocytes. Results The increment of oocyte volume increased the rates of morphologically normal oocytes after sperm injection, which induced much higher rates of overall chromosome detection in bull, ram and dog spermatozoa. The recipient oocyte volume did not affect the chromosomal integrity. Furthermore, in bull, the chromosomal integrity detected by fused mouse oocytes was similar to that derived from a homologous system. On the other hand, chromosomal plates of boar spermatozoa could not be detected despite the use of fused oocytes. Conclusion These data indicate that fused mouse oocytes improved the efficiency of chromosome detection in bull, ram and dog spermatozoa.

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Gender Preselection in Cattle with Intracytoplasmically Injected, Flow Cytometrically Sorted Sperm Heads1

Cited by 87 publications

References 19 publications

A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

Intracytoplasmic sperm injection in domestic and wild mammals

A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes

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