Gender-specific association of oxidative stress and immune response in septic shock mortality using NMR-based metabolomics

Pandey, Swarnima; Siddiqui, Mohd Adnan; Trigun, Surendra Kumar; Azim, Afzal; Sinha, Neeraj

doi:10.1039/d1mo00398d

Cited by 5 publications

(8 citation statements)

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“…The criteria used for generating the final list of significant metabolites were VIP (variable importance of projection) score >1, Boneferrroni-corrected p-value <0.05, and FDR < 0.05. 27 The VIP score measures the significance of each variable in the projection used in the OPLS-DA model. The p-value is the probability that in the study, the observed effect has occurred by chance.…”

Section: Discussionmentioning

confidence: 99%

“…57 Isobutyric acid, a short-chain saturated fatty acid and a product of protein catabolism, is elevated in kidney injury and is considered an organ failure-related metabolite that affects patients. 20,27,58 Valine, a marker for increased severity of ARDS, indicates a shift from the usual energy production pathways to alternative resources. It is a product of muscle breakdown and is expected in patients with acute lung injuries.…”

Section: Discussionmentioning

confidence: 99%

“…The samples were left to clot for 30 min at room temperature before being centrifuged at 2000 × g for 10 min to separate the serum. The serum was then transferred into sterile Eppendorf tubes with appropriate labeling and preserved at −80 °C for subsequent analysis …”

Section: Methodsmentioning

confidence: 99%

“…The serum was then transferred into sterile Eppendorf tubes with appropriate labeling and preserved at −80 °C for subsequent analysis. 27…”

Section: Introductionmentioning

confidence: 99%

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Unveiling Pathophysiological Insights: Serum Metabolic Dysregulation in Acute Respiratory Distress Syndrome Patients with Acute Kidney Injury

Singh,

Siddiqui,

Pandey

et al. 2024

J. Proteome Res.

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Acute respiratory distress syndrome (ARDS) is associated with high mortality rates, which are further exacerbated when accompanied by acute kidney injury (AKI). Presently, there is a lack of comprehensive studies thoroughly elucidating the metabolic dysregulation in ARDS patients with AKI leading to poor outcomes. We hypothesized that metabolomics can be a potent tool to highlight the differences in the metabolic profile unraveling unidentified pathophysiological mechanisms of ARDS patients with and without AKI. 1 H nuclear magnetic resonance spectroscopy was used to identify key metabolites in the serum samples of 75 patients. Distinct clusters of both groups were obtained as the study's primary outcome using multivariate analysis. Notable alternations in the levels of nine metabolites were identified. Pathway analysis revealed the dysregulation of five significant cycles, which resulted in various complications, such as hyperammonemia, higher energy requirements, and mitochondrial dysfunction causing oxidative stress. Identified metabolites also showed a significant correlation with clinical scores, indicating severity. This study shows the alterations in the metabolite concentration highlighting the difference in the pathophysiology of both patient groups and its association with outcome, pointing in the direction of a personalized medicine approach and holding significant promise for application in critical care settings to improve clinical outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The serum was then transferred into sterile Eppendorf tubes with appropriate labeling and preserved at −80 °C for subsequent analysis. 27…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Unveiling Pathophysiological Insights: Serum Metabolic Dysregulation in Acute Respiratory Distress Syndrome Patients with Acute Kidney Injury

Singh,

Siddiqui,

Pandey

et al. 2024

J. Proteome Res.

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“…Works by Pandey et al illustrated the metabolic alteration in patients with sepsis and septic shock due to the presence of comorbid conditions like diabetes and hypertension 98 , gender 99 , and alteration in metabolic profile during disease or progression of the disease 100 , 101 .…”

Section: Metabolomicsmentioning

confidence: 99%

Sepsis, Management & Advances in Metabolomics

Pandey

2024

Nanotheranostics

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Though there have been developments in clinical care and management, early and accurate diagnosis and risk stratification are still bottlenecks in septic shock patients. Since septic shock is multifactorial with patient-specific underlying co-morbid conditions, early assessment of sepsis becomes challenging due to variable symptoms and clinical manifestations. Moreover, the treatment strategies are traditionally based on their progression and corresponding clinical symptoms, not personalized. The complex pathophysiology assures that a single biomarker cannot identify, stratify, and describe patients affected by septic shock. Traditional biomarkers like CRP, PCT, and cytokines are not sensitive and specific enough to be used entirely for a patient's diagnosis and prognosis. Thus, the need of the hour is a sensitive and specific biomarker after comprehensive analysis that may facilitate an early diagnosis, prognosis, and drug development. Integration of clinical data with metabolomics would provide means to understand the patient's condition, stratify patients better, and predict the clinical outcome.

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Metabolomics for the identification of biomarkers in endometriosis

Pandey

2024

Arch Gynecol Obstet

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Gender-specific association of oxidative stress and immune response in septic shock mortality using NMR-based metabolomics

Abstract: Background: Sepsis and septic shock are still associated with a high mortality rate. Early-stage prediction of septic shock outcomes would be helpful to clinicians for designing their treatment protocol. In...

Cited by 5 publications

References 88 publications

Unveiling Pathophysiological Insights: Serum Metabolic Dysregulation in Acute Respiratory Distress Syndrome Patients with Acute Kidney Injury

Unveiling Pathophysiological Insights: Serum Metabolic Dysregulation in Acute Respiratory Distress Syndrome Patients with Acute Kidney Injury

Sepsis, Management & Advances in Metabolomics

Metabolomics for the identification of biomarkers in endometriosis

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