Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure

Yu, Wai-Kin; Shi, Yu; Fong, Chi-Chun; Chen, Y.; Merwe, Jason P. van de; Chan, A. H. C.; Fan, Wei; Bo, Jun; Ye, R.; Au, Doris W.T.; Wu, Rudolf S.S.; Yang, Mengsu

doi:10.1016/j.cbd.2013.06.004

Cited by 8 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These differences of those two hepatic stress markers between sexes might be related to additional energetic and metabolic involvement of the female fish liver in reproductive processes, such as the synthesis of the oocyte contents. Furthermore, liver enzymes such as cytochrome P450, GST, aldehyde dehydrogenase, and hydroxysteroid dehydrogenase, were differentially expressed between the two sexes, underlying the sexual differences in the xenobiotic detoxification capabilities. − …”

Section: Resultsmentioning

confidence: 99%

Gender-Specific Toxicological Effects of Chronic Exposure to Pure Microcystin-LR or Complex Microcystis aeruginosa Extracts on Adult Medaka Fish

Manach

Khenfech

Huet

et al. 2016

Environ. Sci. Technol.

View full text Add to dashboard Cite

Cyanobacterial blooms often occur in freshwater lakes and constitute a potential health risk to human populations, as well as to other organisms. However, their overall and specific implications for the health of aquatic organisms that are chronically and environmentally exposed to cyanobacteria producing hepatotoxins, such as microcystins (MCs), together with other bioactive compounds have still not been clearly established and remain difficult to assess. The medaka fish was chosen as the experimental aquatic model for studying the cellular and molecular toxicological effects on the liver after chronic exposures (28 days) to environmentally relevant concentrations of pure MC-LR, complex extracts of MC producing or nonproducing cyanobacterial biomasses, and of a Microcystis aeruginosa natural bloom. Our results showed a higher susceptibility of females to the different treatments compared to males at both the cellular and the molecular levels. Although hepatocyte lysis increased with MC-containing treatments, lysis always appeared more severe in the liver of females compare to males, and the glycogen cellular reserves also appeared to decrease more in the liver of females compared to those in the males. Proteomic investigations reveal divergent responses between males and females exposed to all treatments, especially for proteins involved in metabolic and homeostasis processes. Our observations also highlighted the dysregulation of proteins involved in oogenesis in female livers. These results suggest that fish populations exposed to cyanobacteria blooms may potentially face several ecotoxicological issues.

show abstract

Section: Resultsmentioning

confidence: 99%

Gender-Specific Toxicological Effects of Chronic Exposure to Pure Microcystin-LR or Complex Microcystis aeruginosa Extracts on Adult Medaka Fish

Manach

Khenfech

Huet

et al. 2016

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…For 2-year-old yellow catfish, we have found 12 sex differentially expressed genes in male, including CYP1A, 20BHSD, GTHa, zonadhesin (ZAN) (Hunt et al 2005 ), growth hormone (GH), cytochrome P450 arom, leptin receptor (LEPR) (Liu et al 2010 ; Gong et al 2013 ), Sox9a2 (Nakamoto et al 2009 ), insulin-like growth factor binding protein 1 (IGFBP1) (Yu et al 2013 ), HSP70 (Mayer and Bukau 2005 ; Tao et al 2013 ), RPL15, and myogenin. Only one sex differentially expressed gene was detected in female, which is ppiD (Table 7 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain

Lü

Zheng

et al. 2015

Mar Biotechnol

View full text Add to dashboard Cite

Yellow catfish (Pelteobagrus fulvidraco) is a pivotal freshwater aquaculture species in China. It shows sexual size dimorphism favoring male in growth. Whole transcriptome approach is required to get the overview of genetic toolkit for understanding the sex determination mechanism aiming at devising its monosex production. Beside gonads, the brain is also considered as a major organ for vertebrate reproduction. Transcriptomic analyses on the brain and of different developmental stages will provide the dynamic view necessary for better understanding its sex determination. In this regard, we have performed a de novo assembly of yellow catfish brain transcriptome by high throughput Illumina sequencing. A total number of 154,507 contigs were obtained with the lengths ranging from 201 to 27,822 bp and N50 of 2,101 bp, as well as 20,699 unigenes were identified. Of these unigenes, 13 and 54 unigenes were detected to be XY-specifically expressed genes (SEGs) for one and 2-year-old yellow catfish, while the corresponding numbers of XX-SEGs for those two stages were 19 and 13, respectively. Our work identifies a set of annotated genes that are candidate factors affecting sexual dimorphism as well as simple sequence repeat (SSR) and single nucleotide variation (SNV) in yellow catfish. To validate the expression patterns of the sex-related genes, we performed quantitative real-time PCR (qRT-PCR) indicating the reliability and accuracy of our analysis. The results in our study may enhance our understanding of yellow catfish sex determination and potentially help to improve the production of all-male yellow catfish for aquaculture.Electronic supplementary materialThe online version of this article (doi:10.1007/s10126-015-9650-z) contains supplementary material, which is available to authorized users.

show abstract

“…In our experiments and according to other investigations on small fish ecotoxicology, various hepatotoxic stressors such as cyanotoxins 11 , pesticides 27 28 or aromatic hydrocarbons 29 induce a higher toxicological response in female livers, suggesting that females would be more sensitive to the effects of those molecules than males 15 17 18 . However, some examples also attest to a higher susceptibility of male livers according to certain specific exposure conditions to toxic chemicals 30 , and a careful investigation of the dimorphic detoxification capabilities should aim at being performed for each specific chemical or hepatic stressor evaluation. Indeed, as the liver, being the principal detoxification organ, presents noticeable sex-differences in its drug metabolism ( e.g.…”

Section: Discussionmentioning

confidence: 99%

Deep sexual dimorphism in adult medaka fish liver highlighted by multi-omic approach

Qiao

Manach

Sotton

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Sexual dimorphism describes the features that discriminate between the two sexes at various biological levels. Especially, during the reproductive phase, the liver is one of the most sexually dimorphic organs, because of different metabolic demands between the two sexes. The liver is a key organ that plays fundamental roles in various physiological processes, including digestion, energetic metabolism, xenobiotic detoxification, biosynthesis of serum proteins, and also in endocrine or immune response. The sex-dimorphism of the liver is particularly obvious in oviparous animals, as the female liver is the main organ for the synthesis of oocyte constituents. In this work, we are interested in identifying molecular sexual dimorphism in the liver of adult medaka fish and their sex-variation in response to hepatotoxic exposures. By developing an integrative approach combining histology and different high-throughput omic investigations (metabolomics, proteomics and transcriptomics), we were able to globally depict the strong sexual dimorphism that concerns various cellular and molecular processes of hepatocytes comprising protein synthesis, amino acid, lipid and polysaccharide metabolism, along with steroidogenesis and detoxification. The results of this work imply noticeable repercussions on the biology of oviparous organisms environmentally exposed to chemical or toxin issues.

show abstract

Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure

Cited by 8 publications

References 33 publications

Gender-Specific Toxicological Effects of Chronic Exposure to Pure Microcystin-LR or Complex Microcystis aeruginosa Extracts on Adult Medaka Fish

Gender-Specific Toxicological Effects of Chronic Exposure to Pure Microcystin-LR or Complex Microcystis aeruginosa Extracts on Adult Medaka Fish

Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain

Deep sexual dimorphism in adult medaka fish liver highlighted by multi-omic approach

Contact Info

Product

Resources

About