Workplace harassment is a significant concern across various industries, including academic libraries, where female professionals often encounter unique challenges. This study assesses the prevalence and effect of workplace harassment among female library professionals in Indian academic libraries. 100 female professionals working in college and university libraries across India participated in the survey conducted in May 2024. The study explores the types, contexts, and perpetrators of harassment and its effects on well-being and job performance. Findings reveal a high prevalence of harassment, predominantly perpetrated by colleagues, with significant effects on individuals’ psychological well-being and job satisfaction. While some security measures are in place, perceptions of their effectiveness vary. Recommendations include integrating security measures into policies, enhancing reporting procedures, and providing better support systems for victims. Addressing workplace harassment requires collaborative efforts to foster inclusive and respectful environments, ensuring the safety and dignity of all library professionals.