Gene activation by copy transposition in mating-type switching of a homothallic fission yeast

Egel, Richard; Gütz, Herbert

doi:10.1007/bf00419574

Cited by 72 publications

(31 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This lesion is then repaired by homologous donor sequences, mat2-P and mat3-M, located on the same chromosome. These loci are maintained in a silent chromatin state, preventing their transcription or recombination, but they can serve as donors of genetic information at mat1 by recombination (7,15,25). The MT switching pattern, as determined several years ago by pedigree analyses at the single-cell level, follows two strict rules, as depicted in Fig.…”

mentioning

confidence: 99%

Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe

Holmes

Kaykov

Arcangioli

2005

Molecular and Cellular Biology

View full text Add to dashboard Cite

A strand-specific imprint (break) controls mating-type switching in fission yeast. By introducing a thiamine repressible promoter upstream of the mat1 locus, we can force transcription through the imprinted region, erasing the imprint and inhibiting further mating-type switching, in a reversible manner. Starting from a synchronized, virgin M-cell population, we show that the site-and strand-specific break is formed when DNA replication intermediates appear at mat1 during the first S phase. The formation of the break is concomitant with a replication fork pause and binding of the Swi1 protein at mat1 until early G 2 and then rapidly disappears. Upon its formation, the break remains stable throughout the cell cycle and triggers mating-type switching during the second S phase. Finally, we have recreated the mating-type switching pedigree at the molecular and single-cell levels, allowing for the first time separation between the establishment of imprinting and its developmental fate.The fission yeast Schizosaccharomyces pombe possesses a remarkable system for changing its mating type (MT) (reviewed in references 4 and 13). Homothallic MT switching in fission yeast, similar to that in budding yeast, is a genetically programmed event in haploid cells initiated by a site-specific lesion at the mat1 locus. This lesion is then repaired by homologous donor sequences, mat2-P and mat3-M, located on the same chromosome. These loci are maintained in a silent chromatin state, preventing their transcription or recombination, but they can serve as donors of genetic information at mat1 by recombination (7,15,25). The MT switching pattern, as determined several years ago by pedigree analyses at the single-cell level, follows two strict rules, as depicted in Fig. 1A (17, 28, 34). The first, termed the one-in-four rule, indicates that two consecutive divisions are necessary to produce one switched cell among four granddaughter cells. The first division produces two cells with identical MTs, but with different switching potentials, termed unswitchable (u) and switchable (s), such that only the s cell produces one switched cell after the second division. The second, termed the consecutive rule, indicates that the sister of this switched cell is able to switch during subsequent cell divisions. The newly switched cell is unswitchable, and will follow the same fate as the unswitchable (Mu or Pu) cells (where M stands for minus and P stands for plus). Therefore, an exponential culture-or well-grown colony contains not two but four cell types, with P or M MT information, and with two switching potentials, u and s, found in equal proportions (1 Pu:1 Mu:1 Ps:1 Ms). Cells from opposite MTs are able to conjugate in G 1 and sporulate when grown under sporulating conditions. The integrity of this pedigree and switching is conveniently verified by staining cells within a colony with iodine vapors, a marker of spore-containing colonies, such that wild-type homothallic cells stain darkly, whereas mutant cells exhibit a streaky or white phenotype (3...

show abstract

mentioning

confidence: 99%

Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe

Holmes

Kaykov

Arcangioli

2005

Molecular and Cellular Biology

View full text Add to dashboard Cite

show abstract

“…Their segregation will generate one switched and one nonswitched granddaughter cell. The recently switched cell will again imprint its DNA and will repeat the cycle to produce a single switched (Egel and Gutz, 1981;Beach, 1983;Beach and Klar, 1984;Egel, 1984a;Kelly et al, 1988).…”

mentioning

confidence: 99%

The developmental fate of fission yeast cells is determined by the pattern of inheritance of parental and grandparental DNA strands

Klar¹

1989

Cell Differentiation and Development

View full text Add to dashboard Cite

“…Its general principles also seem applicable to the mating-type interconversion in fission yeast Schizosaccharomyces pombe (Egel and Gutz, 1981).…”

Section: Introductionmentioning

confidence: 99%

Homothallism expression in Kluyveromyces lactis

et al. 1983

View full text Add to dashboard Cite

SUMMARYKinetics of the mating-type interconversion was observed after heterothallic nutritionally-marked stocks of Kluyveromyces lactis were mated and sporulated.Conversion to homothallism is not finished within the period of 10 days of growth on the sporulation agar. In general, the expression of homothallism in the a mating-type is often delayed when compared to the a mating-type.Segregation patterns of tetrads suggest the functioning of a mating-type interconversion system including a mating locus and two silent storage copies of the mating-type information.

show abstract

Gene activation by copy transposition in mating-type switching of a homothallic fission yeast

Cited by 72 publications

References 10 publications

Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe

Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe

The developmental fate of fission yeast cells is determined by the pattern of inheritance of parental and grandparental DNA strands

Homothallism expression in Kluyveromyces lactis

Contact Info

Product

Resources

About