Gene cloning of a thaumatin-like (PR-5) protein of western white pine (Pinus monticola D. Don) and expression studies of members of the PR-5 group

Piggott, Nina; Ekramoddoullah, A.K.M.; Liu, Jun‐Jun; Yu, Xueshu

doi:10.1016/j.pmpp.2004.05.004

Cited by 37 publications

(24 citation statements)

References 22 publications

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“…We have reported previously that malting barley TLPs isoforms are glycosylated with different sugar content, TLP-S being two fold higher and with the ability to bind concanavalin A 8 . It is worthy to note that the number of newly recognized glycoproteins among the TLPs family is growing 13,16,18,19,23,26,37 . We have demonstrated TLP-S has higher antifungal and antibacterial activity than TLP-R, regarding the IC 50 value.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Gorjanović

Beljanski

Gavrović-Jankulović

et al. 2007

Journal of the Institute of Brewing

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Two basic proteins were isolated to homogeneity from malting barley (Hordeum vulgare L.) grain. Proteins were identified as members of a Thaumatin-Like Protein (TLP) family, by Western blot. Isoforms, assigned as TLP-S and TLP-R, have slightly different mobility at about 22 and 27 kDa in nonreducing and reducing conditions, and pI values of 9.5 and 9.4, respectively. The antifungal potency of malting barley grain TLPs isoforms was examined on Micrococcus lysodeikticus, Saccharomyces cerevisiae, Candida albicans and plant pathogen Fusarium sporotrichioides growth in vitro. It was found that that IC 50 value for TLP-S was two fold higher. Antibacterial and antifungal activities of both isoforms were completely abolished by divalent (Ca 2+ , Mn 2+ , Mg 2+ ) and monovalent (K + ) cations, at concentrations approximating physiological ionic strength and higher. Glucanase activity was not observed; neither TLP-S nor TLP-R digested glucan. On the basis of these results, the importance of TLP for barley grain protection against fungal diseases has been discussed together with the mechanism of antimicrobial action.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Gorjanović

Beljanski

Gavrović-Jankulović

et al. 2007

Journal of the Institute of Brewing

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“…For example, one key diVerence between our study and many others studies is the use of a coniferous rather than an herbaceous host. There is increasing evidence to suggest that for conifers, the biochemical and molecular basis of SIR are distinct compared to angiospermous models (Hudgins et al 2004;Piggott. et al 2004;Luchi et al 2005;Blodgett et al 2007).…”

Section: Evects Of Fungal and Herbivore Induction On Fungal Challengementioning

confidence: 99%

Cross-induction of systemic induced resistance between an insect and a fungal pathogen in Austrian pine over a fertility gradient

et al. 2007

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Evidence for cross-induction of systemic resistance or susceptibility in plant-fungus-herbivore interactions is mostly derived from herbaceous model systems and not perennial woody plants. Furthermore, the effects of environmental variables such as soil fertility on these tripartite interactions are generally unknown. This study examined cross-induction of systemic resistance in Pinus nigra (Austrian pine) to infection by Sphaeropsis sapinea (a fungal pathogen), or feeding by Neodiprion sertifer (European pine sawfly), by prior induction with either S. sapinea or N. sertifer, over a fertility gradient. In a replicated 3-year study, cross-induction of systemic induced resistance (SIR) was found to be both asymmetric within a single year and variable between years. Prior induction with insect defoliation induced SIR to subsequent fungal challenge in 2006 but not in 2005. In 2005, a fertility-independent negative systemic effect of the fungal infection on herbivore growth was detected while herbivore survival was affected by a significant interaction between induction treatment and fertility level in 2006. Prior infection by the fungus induced SIR against the same fungus in both years regardless of fertility levels. This is the first report of whole-plant SIR against a defoliating insect induced by a fungal pathogen and vice versa, under variable nutrient availability, in a conifer or any other tree.

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“…Many TLPs have been identified in different organisms, such as eudicots, monocots, gymnosperms, and even some animal species (van Loon 1985;Kitajima and Sato 1999;Anna et al 2004;Piggott et al 2004). The results of genome-wide analysis showed that TLPs occur in plants as a multiple gene family, such as in Arabidopsis thaliana and Oryza sativa (Shatters et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa

Zhao

2010

Planta

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Some pathogenesis-related proteins (PR proteins) are subject to positive selection, while others are under negative selection. Here, we report the patterns of molecular evolution in thaumatin-like protein (TLP, PR5 protein) genes of Populus trichocarpa. Signs of positive selection were found in 20 out of 55 Populus TLPs using the likelihood ratio test and ML-based Bayesian methods. Due to the connection between the acidic cleft and the antifungal activity, the secondary structure and three-dimensional structure analyses predicted antifungal activity beta-1,3-glucanase activities in these TLPs. Moreover, the coincidence with variable basic sites in the acidic cleft and positively selected sites suggested that fungal diseases may have been the main environmental stress that drove rapid adaptive evolution in Populus.

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Gene cloning of a thaumatin-like (PR-5) protein of western white pine (Pinus monticola D. Don) and expression studies of members of the PR-5 group

Cited by 37 publications

References 22 publications

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Cross-induction of systemic induced resistance between an insect and a fungal pathogen in Austrian pine over a fertility gradient

Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa

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