Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae

Wegner, Alex; Casanova, Florencia; Loehrer, Marco; Jordine, Angelina; Bohnert, Stefan; Liu, Xinyu; Schaffrath, Ulrich

doi:10.1007/s12275-022-1074-7

Cited by 15 publications

(12 citation statements)

References 42 publications

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“…This indicated that FsPL may participate in the vegetative growth of pathogenic fungi, as well as in the degradation of host pectin by pathogen fungi, but not in the regulation of sporulation. Similarly, deletion of the pectate lyase gene MoPL1 affected the growth of Magnaporthe oryzae ( 32 ), while deletion of the pectate lyase gene VdPL1-4 affected neither the spore morphology nor the spore production of Verticillium dahliae ( 33 ). In vitro and in vivo inoculation experiments showed that the virulence of the FsPL knockout mutant was significantly reduced compared to wild-type F. sacchari .…”

Section: Discussionmentioning

confidence: 99%

Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence

et al. 2023

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“…8 ). Although PELs have been extensively reported to be involved in pathogenesis in phytopathogenic fungi ( 19 , 21 – 26 ), the role of PEL16 in pathogenicity is rarely reported. In P. capsici , the pectate lyase gene PcPL16 showed strong expression levels during infection, and overexpressing PcPL16 triggered strong cell death in pepper and tobacco leaves ( 25 ).…”

Section: Discussionmentioning

confidence: 99%

“…In another study, 22 PEL genes in Phytophthora capsici were cloned, and the combined silencing of PcPL1 , PcPL15 , PcPL16 , and PcPL20 severely affected virulence on pepper ( 25 ). Gene deletion and constitutive expression of pectate lyase gene 1 resulted in diminished virulence of Magnaporthe oryzae ( 26 ). However, the biological functions of CcPEL s in C. camelliae have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of Colletotrichum camelliae and CcPEL16 Is Required for Full Virulence to Tea Plants

Jiang

Cao

Wang

et al. 2023

mSphere

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In this study, we first established a PEG-mediated protoplast transformation system of C. camelliae and used it to investigate the biological functions of seven pectate lyase genes ( CcPEL s) which were abundantly expressed during infection. The results provided insights into the contributions of pectate lyase to mycelial growth, conidial production, appressorium formation, and the pathogenicity of C. camelliae. We also confirmed the secretory function of CcPEL proteins and their role in suppressing Bax-induced cell death.

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“…To verify the reliability of the selected reference genes. Pectate lyase 31 , an enzyme involved in the digestion of the cell wall of pitaya, was analyzed using RT-qPCR in the early stages of N. dimidiatum infection of pitaya. The specific primers used are shown in Table 1.…”

Section: Validation Of Reference Genesmentioning

confidence: 99%

Identification of RT-qPCR reference genes suitable for gene function studies in the pitaya canker disease pathogen Neoscytalidium dimidiatum

Wang

Sang

et al. 2022

Sci Rep

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Neoscytalidium dimidiatum is the main causal agent of pitaya canker. Most studies of virulence and pathogenicity genes have measured expression levels using real-time quantitative polymerase chain reaction (RT-qPCR). Suitable reference genes are essential for ensuring that estimates of gene expression levels by RT-qPCR are accurate. However, no reference genes can be robustly applied across all contexts and species. No studies to date have evaluated the most effective reference genes for normalizing gene expression levels estimated by RT-qPCR in N. dimidiatum. In this study, RT-qPCR data for individual candidate reference genes were analyzed using four different methods: the delta Ct method and the geNorm, NormFinder, and BestKeeper algorithms. We evaluated the utility of eight candidate reference genes (18S rRNA, Actin (1), Actin (2), Actin, GAPDH (1), GAPDH (2), UBQ, and Tubulin) for normalizing expression levels estimated by RT-qPCR in N. dimidiatum at different developmental stages, at different temperatures, and during interaction with pitaya. All candidate reference genes were suitable for gene expression analysis except for Actin (2). Tubulin and Actin (1) were the most stably expressed reference genes under different temperatures. Actin (1) and Actin were the most stably expressed reference genes in N. dimidiatum at different developmental stages. Tubulin and UBQ were the most stably expressed reference genes during interaction with pitaya. Actin and 18s rRNA were the most stably expressed across all experimental conditions. Subsequently, Tubulin and UBQ were further investigated in analyses of pectinase expression during the pitaya–N. dimidiatum interaction. Our results provide insights that will aid future RT-qPCR studies of gene expression in N. dimidiatum.

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Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae

Cited by 15 publications

References 42 publications

Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence

Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence

Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of Colletotrichum camelliae and CcPEL16 Is Required for Full Virulence to Tea Plants

Identification of RT-qPCR reference genes suitable for gene function studies in the pitaya canker disease pathogen Neoscytalidium dimidiatum

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