Gene delivery in conjunction with gold nanoparticle and tumor treating electric field

Tiwari, Pawan Kumar; Lee, Yeon Soo

doi:10.1063/1.4817090

Cited by 19 publications

(12 citation statements)

References 49 publications

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“…Also, the plot in Figure 9(b) shows the relative percentage of light scattering of the average particle size and the distinct peak was found at 91.28 nm, which indicates the presence of nanoscale aggregates within the samples. This result was compared to that found in the literature and was found to be similar [66,67].…”

Section: Dynamic Light Scattering (Dls) and Zeta Potentialsupporting

confidence: 59%

Nanogold for In Vitro Inhibition of Salmonella Strains

Boatemaa

Ragunathan

Naskar

2019

Journal of Nanomaterials

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The pathogenic strains of Salmonella typhi, paratyphi, and typhimurium are the major cause of typhoid and food poisoning in children and adults in developing countries. According to WHO estimation, 22 million cases of typhoid fever and 200,000 related deaths occur worldwide each year with an additional 6 million cases of paratyphoid fever estimated to occur annually with the highest incidence in children, resulting in a high death rate. The high use of antibiotics has also given rise to drug-resistant strains. Hence, it was of importance to assess the inhibition and quick detection of pathogenic strains of Salmonella. This study aims to investigate the chemically synthesized gold nanoparticles (GNPs) for its antibacterial activity against clinical isolates of S. typhi and S. paratyphi including food sample isolates. The GNPs were characterized using visible color change, UV-Vis spectrophotometry, FTIR, XRD, DLS, FESEM, TEM, and zeta potential. The plasmon peak at 525 nm and 535 nm confirmed the synthesis of gold nanoparticles. The size of the chemically synthesized gold nanoparticles (GNPs) were in the range of 40-60 nm, while FESEM and TEM images revealed that the GNPs were spherical in shape. For antimicrobial activities, five of the Salmonella strains were isolated from fish and egg samples, while the other seven were S. typhi and S. paratyphi from clinical samples. The inhibition factor for GNPs showed higher inhibition against S. paratyphi, while the inhibition factor for S. typhi were found to be higher than Ciprofloxacin-30. This is the first study of the antibacterial efficacy of GNPs against pathogenic strains of Salmonella. The obtained results suggest that nanobioconjugated gold may be of interest in the detection of typhoid and high potential use in areas in biomedicine as an alternative to antibiotics.

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Section: Dynamic Light Scattering (Dls) and Zeta Potentialsupporting

confidence: 59%

Nanogold for In Vitro Inhibition of Salmonella Strains

Boatemaa

Ragunathan

Naskar

2019

Journal of Nanomaterials

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“…The NPs are capable of attaching to the bacterial membrane by electrostatic interaction and disrupt its integrity [24]. They can change membrane potential and decrease ATP levels within the cell and inhibit the binding of tRNA with ribosomal subunit, affecting translation [25].…”

Section: Mechanism Of Antibacterial Activity Of Gold Nanoparticlesmentioning

confidence: 99%

Gold Nanoparticles: Their Application as i Antimicrobial Agents and Vehicles of Gene Delivery

Kundu¹

2017

AIBM

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“…HER2 over-expression leads to cancerous cell proliferation and is seen in cancers such as breast cancer, ovarian cancer, stomach cancer, and even lung cancer 27 , and both SKOV3 and MCF7 cells over-express HER2 markers on their surface membrane. It has also been shown that in the presence of an electric field, AuNPs could change the distribution of the field within the cell's cytoplasm resulting in an inward net electric force which potentially could change the function of the cell 28 . Thus, further experiments specifically targeting the two types of cancer cells using HER2 antibody functionalized gold nanoparticles (HER2-AuNPs) were performed to determine if enhanced electric field strength can be induced via the application of nanoparticles, consequently leading to the killing of the cancerous cells without affecting non cancerous HUVECs and MCF10a providing a platform for the development of a non-invasive cancer treatment without any harmful side effects.…”

Section: Introductionmentioning

confidence: 99%

Immuno Nanoparticles Integrated Electrical Control of Targeted Cancer Cell Development Using Whole Cell Bioelectronic Device

Hondroulis¹,

Zhang²,

Zhang³

et al. 2014

Theranostics

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Electrical properties of cells determine most of the cellular functions, particularly ones which occur in the cell's membrane. Manipulation of these electrical properties may provide a powerful electrotherapy option for the treatment of cancer as cancerous cells have been shown to be more electronegative than normal proliferating cells. Previously, we used an electrical impedance sensing system (EIS) to explore the responses of cancerous SKOV3 cells and normal HUVEC cells to low intensity (<2 V/cm) AC electric fields, determining that the optimal frequency for SKOV3 proliferation arrest was 200 kHz, without harming the non-cancerous HUVECs. In this study, to determine if these effects are cell type dependant, human breast adenocarcinoma cells (MCF7) were subjected to a range of frequencies (50 kHz-2 MHz) similar to the previously tested SKOV3. For the MCF7, an optimal frequency of 100 kHz was determined using the EIS, indicating a higher sensitivity towards the applied field. Further experiments specifically targeting the two types of cancer cells using HER2 antibody functionalized gold nanoparticles (HER2-AuNPs) were performed to determine if enhanced electric field strength can be induced via the application of nanoparticles, consequently leading to the killing of the cancerous cells without affecting non cancerous HUVECs and MCF10a providing a platform for the development of a non-invasive cancer treatment without any harmful side effects. The EIS was used to monitor the real-time consequences on cellular viability and a noticeable decrease in the growth profile of the MCF7 was observed with the application of the HER2-AuNPs and the electric fields indicating specific inhibitory effects on dividing cells in culture. To further understand the effects of the externally applied field to the cells, an Annexin V/EthD-III assay was performed to determine the cell death mechanism indicating apoptosis. The zeta potential of the SKOV3 and the MCF7 before and after incorporation of the HER2-AuNPs was also obtained indicating a decrease in zeta potential with the incorporation of the nanoparticles. The outcome of this research will improve our fundamental understanding of the behavior of cancer cells and define optimal parameters of electrotherapy for clinical and drug delivery applications.

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Gene delivery in conjunction with gold nanoparticle and tumor treating electric field

Cited by 19 publications

References 49 publications

Nanogold for In Vitro Inhibition of Salmonella Strains

Nanogold for In Vitro Inhibition of Salmonella Strains

Gold Nanoparticles: Their Application as i Antimicrobial Agents and Vehicles of Gene Delivery

Immuno Nanoparticles Integrated Electrical Control of Targeted Cancer Cell Development Using Whole Cell Bioelectronic Device

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