Gene delivery targeted to the brain using an Angiopep-conjugated polyethyleneglycol-modified polyamidoamine dendrimer

Ke, Weilun; Shao, Kun; Huang, Rongqin; Han, Liang; Liu, Yang; Li, Jianfeng; Kuang, Yuyang; Ye, Liya; Lou, Jinning; Jiang, Chen

doi:10.1016/j.biomaterials.2009.08.049

Cited by 314 publications

(199 citation statements)

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“…The breadth across applications is attributed to dendrimers being well-defined, monodisperse nanostructures with tunable critical nanoscale design parameters [15,16], such as size, shape, rigidity, surface functionality, and solvent affinity. For this reason, dendrimers have been studied as hosts for a number of guest species: drugs [1][2][3][4], gene [17][18][19], contrast agents [7,20,21], metal ions [9][10][11][12][13][14], polymers [22], and organic pollutants [23,24]. Astruc and coworkers have written an exceptionally comprehensive review on dendrimers functioning as hosts [25].…”

Section: Introductionmentioning

confidence: 99%

Multiscale Modeling for Host-Guest Chemistry of Dendrimers in Solution

Kim

Lamm

2012

Polymers

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Dendrimers have been widely used as nanostructured carriers for guest species in a variety of applications in medicine, catalysis, and environmental remediation. Theory and simulation methods are an important complement to experimental approaches that are designed to develop a fundamental understanding about how dendrimers interact with guest molecules. This review focuses on computational studies aimed at providing a better understanding of the relevant physicochemical parameters at play in the binding and release mechanisms between polyamidoamine (PAMAM) dendrimers and guest species. We highlight recent contributions that model supramolecular dendrimer-guest complexes over the temporal and spatial scales spanned by simulation methods ranging from all-atom molecular dynamics to statistical field theory. The role of solvent effects on dendrimer-guest interactions and the importance of relating model parameters across multiple scales is discussed.

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Section: Introductionmentioning

confidence: 99%

Multiscale Modeling for Host-Guest Chemistry of Dendrimers in Solution

Kim

Lamm

2012

Polymers

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“…Peptides targeting the low-density lipoprotein receptor-related protein-1 (LRP1), such as angiopep-2, possess high brain penetration capability and exhibit high transcytosis capacity and parenchymal accumulation. They have been used to decorate dendrimers to increase their BBB crossing ability, leading to a high expression of the target gene in the brain and the spleen [63].…”

Section: Nanoparticles For Brain-specific Drug and Genetic Materials Delmentioning

confidence: 99%

Nanoparticles for Brain-Specific Drug and Genetic Material delivery, Imaging and Diagnosis

Posadas

Monteagudo

Ceña

2016

Nanomedicine (Lond.)

108

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The poor access of therapeutic drugs and genetic material into the central nervous system due to the presence of the blood-brain barrier often limits the development of effective noninvasive treatments and diagnoses of neurological disorders. Moreover, the delivery of genetic material into neuronal cells remains a challenge because of the intrinsic difficulty in transfecting this cell type. Nanotechnology has arisen as a promising tool to provide solutions for this problem. This review will cover the different approaches that have been developed to deliver drugs and genetic material efficiently to the central nervous system as well as the main nanomaterials used to image the central nervous system and diagnose its disorders.

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“…Afterwards, the cell pellet was resuspended in PBS, and fluorescence was measured by a flow cytometer (FACSCalibur, BD Biosciences, Bedford, MA) equipped with an argon ion laser (488 nm). Data were analyzed using WinMDI 2.9 (Microsoft, Redmond, WA) (Ke et al, 2009). The cells cultured under normal conditions and live cells were considered as a negative control and gate, respectively; this means that the only cells at the gate were analyzed.…”

Section: Cellular Uptake Of Dendrimersmentioning

confidence: 99%

“…The cell line MDA-MB231 was cultured at a density of 10 000 cells per well in the 35 mm glassbottom culture dish at 37 C, 5% Co 2 , for 48 h. Afterwards, the cells were incubated with the hPAMAM-PEGDGA complex (5 mg pDNA/well), washed with cold PBS, and finally incubated with 50 nM Lyso Tracker Green and DAPI for 15 and 60 min, respectively. Henceforth, the cells were washed three times with cold PBS, fixed with 3.7% paraformaldehyde (Ke et al, 2009) and observed under the confocal laser scanning microscopy (BioRad MRC1024 MP, CA), with a scan head on a Nikon E800 microscope (60x objective). Figure 1 shows the chemical structure of the DGA-PEG copolymer and hPAMAM.…”

Section: Confocal Microscopymentioning

confidence: 99%