Gene electrotransfer: from biophysical mechanisms to in vivo applications

Escoffre, Jean‐Michel; Mauroy, Chloé; Portet, Thomas; Wasungu, Luc; Rosazza, Chrystelle; Gilbart, Yoann; Mallet, Laetitia; Bellard, Elisabeth; Golzio, Muriel; Rols, Marie‐Pierre

doi:10.1007/s12551-009-0022-7

Cited by 9 publications

(4 citation statements)

References 66 publications

(89 reference statements)

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“…The logarithm of the transfection depends linearly on the logarithm of the pulse duration T [172]. Therefore, the gene expression can be written, for field conditions not affecting to large extend the cell viability, [118, 172, 213]:…”

Section: Gene Electrotransfermentioning

confidence: 99%

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Gene Electrotransfer: A Mechanistic Perspective

Rosazza¹,

Meglič²,

Zumbusch³

et al. 2016

CGT

Self Cite

181

142

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Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability. Although gene electrotransfer has been extensively studied in in vitro and in vivo environments, the mechanisms by which DNA enters and navigates through cells are not fully understood. Here we present a comprehensive review of the body of knowledge concerning gene electrotransfer that has been accumulated over the last three decades. For that purpose, after briefly reviewing the medical applications that gene electrotransfer can provide, we outline membrane electropermeabilization, a key process for the delivery of DNA and smaller molecules. Since gene electrotransfer is a multipart process, we proceed our review in describing step by step our current understanding, with particular emphasis on DNA internalization and intracellular trafficking. Finally, we turn our attention to in vivo testing and methodology for gene electrotransfer.

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Section: Gene Electrotransfermentioning

confidence: 99%

“…( b ) DNA interacts with cationic lipids (darker grey) at the pore edges and via coalescence of the pores DNA passes through the membrane. From [213]. …”

Section: Tablementioning

confidence: 99%

Gene Electrotransfer: A Mechanistic Perspective

Rosazza¹,

Meglič²,

Zumbusch³

et al. 2016

CGT

Self Cite

181

142

View full text Add to dashboard Cite

show abstract

“…In comparison to traditional injection methods, GET not only significantly enhances the number of transfected cells and the intensity of gene expression but also enables co-trans- fection, resulting in a dramatic increase in gene expression levels by 100 to 1000-fold. 119,120 These advantages have positioned GET as a highly promising novel gene delivery tool with immense potential in the field of gene therapy.…”

Section: Electroporation For Gene Therapy In Clinical Settingsmentioning

confidence: 99%

Advanced micro/nano-electroporation for gene therapy: recent advances and future outlook

Liu,

Su,

Jiang

et al. 2024

Nanoscale

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“…Electroporation is a delivery method based on the application of electrical pulses to cells and tissues to destabilize the cell membrane and facilitate the entry of molecules. When nucleic acids are introduced into the cells and tissues, the application is called gene 2 of 25 electrotransfer [4,5]. Another biomedical application of electroporation, in which cytotoxic drugs are introduced into tumours, is called electrochemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Gene Immunotherapy of Colon Carcinoma with IL-2 and IL-12 Using Gene Electrotransfer

Komel,

Omerzel,

Kamensek

et al. 2023

IJMS

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Gene immunotherapy has become an important approach in the treatment of cancer. One example is the introduction of genes encoding immunostimulatory cytokines, such as interleukin 2 and interleukin 12, which stimulate immune cells in tumours. The aim of our study was to determine the effects of gene electrotransfer of plasmids encoding interleukin 2 and interleukin 12 individually and in combination in the CT26 murine colon carcinoma cell line in mice. In the in vitro experiment, the pulse protocol that resulted in the highest expression of IL-2 and IL-12 mRNA and proteins was used for the in vivo part. In vivo, tumour growth delay and also complete response were observed in the group treated with the plasmid combination. Compared to the control group, the highest levels of various immunostimulatory cytokines and increased immune infiltration were observed in the combination group. Long-term anti-tumour immunity was observed in the combination group after tumour re-challenge. In conclusion, our combination therapy efficiently eradicated CT26 colon carcinoma in mice and also generated strong anti-tumour immune memory.

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Gene electrotransfer: from biophysical mechanisms to in vivo applications

Cited by 9 publications

References 66 publications

Gene Electrotransfer: A Mechanistic Perspective

Gene Electrotransfer: A Mechanistic Perspective

Advanced micro/nano-electroporation for gene therapy: recent advances and future outlook

Gene Immunotherapy of Colon Carcinoma with IL-2 and IL-12 Using Gene Electrotransfer

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