Gene expression analysis of maize seedlings (DKB240 variety) inoculated with plant growth promoting bacterium Herbaspirillum seropedicae

Amaral, Fernanda Plucani do; Bueno, Jéssica Cavalheiro Ferreira; Hermes, Vanessa Stahl; Arisi, Ana Carolina Maisonnave

doi:10.1007/s13199-014-0270-6

Cited by 36 publications

(16 citation statements)

References 57 publications

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“…However, their diversity was highest in treatments PGPB and HA + PGPB. A significant increase in transcript levels of ent-kaurene oxidase genes that are involved in gibberellin synthesis occurs in maize seedlings treated with H. seropedicae [38]. The agreement between gene expression in the gibberellin biosynthesis pathway (Zmko1) and the exudation profile in the same biological model supports our suggestion that the gibberellin synthesis pathway in root tissues is modulated by H. seropedicae colonization.…”

Section: Discussionsupporting

confidence: 80%

Root exudate profiling of maize seedlings inoculated with Herbaspirillum seropedicaeand humic acids

Lima

Olivares

Oliveira

et al. 2014

Chem. Biol. Technol. Agric.

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Background: Co-inoculation of maize with Herbaspirillum seropedicae and humic substances increases the sizes of plant-associated bacterial populations and enhances grain yields under laboratory and field conditions. Root exudation is a key mechanism in the regulation of plant-bacterial interactions in the rhizosphere; humic matter supplementation is known to change the exudation of H + ions and organic acids from maize roots. Our starting premise was that H. seropedicae and humic acids would modify maize seedling exudation profiles. We postulated that a better understanding of these shifts in exudate profiles might be useful in improving the chemical environment to promote better performance of plant growth-promoting bacteria delivered as bioinoculants. Thus, root exudates of maize were collected and analyzed by gas chromatography-mass spectrometry (GC-MS) and proton nuclear magnetic resonance (

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Section: Discussionsupporting

confidence: 80%

Root exudate profiling of maize seedlings inoculated with Herbaspirillum seropedicaeand humic acids

Lima

Olivares

Oliveira

et al. 2014

Chem. Biol. Technol. Agric.

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“…On the other hand, the observed overall increase in transcripts level at 7 DAI could be related to the moment in which plant defense responses here analyzed reached their maximum expression level in aerial tissues in response to root bacterial colonization. The subsequent decrease observed at 15 DAI could indicate that the presence of this endophytic bacterium in roots is somehow recognized as “non-hostile.” Therefore, initially-induced defense responses are eventually modulated/attenuated allowing the establishment of this beneficial association, as reported elsewhere (Plucani do Amaral et al, 2014). …”

Section: Discussionsupporting

confidence: 58%

The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots

et al. 2014

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Pseudomonas fluorescens PICF7, a native olive root endophyte and effective biocontrol agent (BCA) against Verticillium wilt of olive, is able to trigger a broad range of defense responses in root tissues of this woody plant. In order to elucidate whether strain PICF7 also induces systemic defense responses in above-ground organs, aerial tissues of olive plants grown under non-gnotobiotic conditions were collected at different time points after root bacterization with this endophytic BCA. A suppression subtractive hybridization (SSH) cDNA library, enriched in up-regulated genes, was generated. This strategy enabled the identification of 376 ESTs (99 contigs and 277 singlets), many of them related to response to different stresses. Five ESTs, involved in defense responses, were selected to carry out time-course quantitative real-time PCR (qRT-PCR) experiments aiming to: (1) validate the induction of these genes, and (2) shed light on their expression pattern along time (from 1 to 15 days). Induction of olive genes potentially coding for lipoxygenase 2, catalase, 1-aminocyclopropane-1-carboxylate oxidase, and phenylananine ammonia-lyase was thus confirmed at some time points. Computational analysis also revealed that different transcription factors were up-regulated in olive aerial tissues (i.e., JERF, bHLH, WRKY), as previously reported for roots. Results confirmed that root colonization by this endophytic bacterium does not only trigger defense responses in this organ but also mounts a wide array of systemic defense responses in distant tissues (stems, leaves). This sheds light on how olive plants respond to the “non-hostile” colonization by a bacterial endophyte and how induced defense response can contribute to the biocontrol activity of strain PICF7.

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“…The endophytic colonization of rice plants with positive growth responses lends credence to the idea that these endophytes are indeed symbionts. Several studies have shown growth promotion in different crops such as rice, maize, wheat, sugarcane, and switchgrass (Panicum virgatum L.) through BNF mediated by diazotrophic endophytes (Amaral et al, 2014;Kim et al, 2012;Iniguez et al, 2004;Momose et al, 2009;James et al, 2002). To our knowledge, this is the first evidence to show growth promotion in rice by poplar and willow endophytes.…”

Section: Discussionmentioning

confidence: 51%

“…strain B50 showed the community of endophytes in intercellular spaces in roots, stems, and leaves, sites of lateral root emergence, the cavities of root aerenchyma, and xylem vessels in leaf sheaths and stems (Gyaneshwar et al, 2001;Singh et al, 2009;Govindarajan et al, 2008;Egener et al, 1999;Verma et al, 2001;Elbeltagy et al, 2001). Several studies have shown growth promotion in different crops such as rice, maize, wheat, sugarcane, and switchgrass (Panicum virgatum L.) through BNF mediated by diazotrophic endophytes (Amaral et al, 2014;Kim et al, 2012;Iniguez et al, 2004;Momose et al, 2009;James et al, 2002). Rahnella sp.…”

Section: Discussionmentioning

confidence: 99%

Diazotrophic Endophytes of Poplar and Willow for Growth Promotion of Rice Plants in Nitrogen‐Limited Conditions

et al. 2015

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Rice (Oryza sativa L.) is one of the most important staple food crops. Its cultivation requires a relatively high input of N fertilizers; however, rice plants do not absorb a significant proportion of added fertilizers, resulting in soil and water pollution. The use of diazotrophic (N‐fixing) endophytes can provide benefits for rice cultivation by reducing the demand of N fertilizers. Diazotrophic endophytes from the early successional plant species poplar (Populus trichocarpa Torr. & A. Gray) and willow (Salix sitchensis C. A. Sanson ex Bong.) were added to rice seedlings. Inoculated rice plants were grown in N‐limited conditions in the greenhouse, and plant physical characteristics were assessed. Endophyte‐inoculated rice plants had greater biomass, higher tiller numbers, and taller plant stature than mock‐inoculated controls. Endophyte populations were quantified and visualized in planta within rice plants using fluorescent microscopy. The endophytes colonized rice plants effectively in both roots and foliage. These results demonstrated that diazotrophic endophytes of the eudicots poplar and willow can colonize rice plants and enhance plant growth in N‐limited conditions.

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Gene expression analysis of maize seedlings (DKB240 variety) inoculated with plant growth promoting bacterium Herbaspirillum seropedicae

Cited by 36 publications

References 57 publications

Root exudate profiling of maize seedlings inoculated with Herbaspirillum seropedicaeand humic acids

Root exudate profiling of maize seedlings inoculated with Herbaspirillum seropedicaeand humic acids

The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots

Diazotrophic Endophytes of Poplar and Willow for Growth Promotion of Rice Plants in Nitrogen‐Limited Conditions

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