Gene expression analysis of Populus deltoides roots subjected to copper stress

Guerra, Fernando; Duplessis, Sébastien; Kohler, Annegret; Martin, Francis; Tapia, Jaime; Lebed, Pablo J.; Zamudio, Francisco; González, Enrique

doi:10.1016/j.envexpbot.2009.08.004

Cited by 31 publications

(17 citation statements)

References 54 publications

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“…Photosynthetic tissues are known to be especially sensitive to Cu-induced oxidative stress occurring as a result of the inhibition of photosynthetic electron transport (Ratkevicius et al 2003;Kalinowska and Pawlik-Skowrońska 2010) and a higher content of MDA recorded in the leaves of our Cu-treated plants than in control further support this hypothesis. Moreover, according to Guerra et al (2009), leaf necrosis in Cu-treated plants may be observed even in the absence of obvious Cu accumulation within leaf tissues and could occur as a result of long-distance signalling involving ethylene and abscisic acid.…”

Section: Discussionmentioning

confidence: 99%

Effects of Salinity on the Response of the Wetland Halophyte Kosteletzkya virginica (L.) Presl. to Copper Toxicity

Han

Lefèvre

Ruan

et al. 2011

Water Air Soil Pollut

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Kosteletzkya virginica (L.) Presl. is a perennial wetland halophyte which could be exposed to heavy metals in polluted salt marsh environments. In order to investigate the interaction between salinity (50 mM NaCl) and heavy metal, young plants were exposed in hydroponic culture to 10 μM Cu in the presence or absence of 50 mM NaCl. Copper strongly inhibited the leaf emergence and lateral branch development as well as leaf expansion, and induced a significant decrease in plant dry weight (DW), water content (WC), osmotic potential (Ψ s ) and leaf water potential (Ψ w ). Copper treated plants accumulated significantly higher level of Cu in the roots than in the shoots although Cu intake rates decreased with the duration of stress exposure. Additions of NaCl in the absence of Cu excess had no detrimental impact on plant growth. In the presence of Cu excess, NaCl decreased Cu accumulation in roots and stems but had no positive impact on plant growth. Copper induced a large decrease in K concentration in roots and stems as well as a decrease in Ca concentration in the leaves. The impact of Cu and NaCl appeared to be additive on leaf Ψ s and leaf quaternary ammonium compounds concentrations. It is concluded that K. virginica exhibits a high bioconcentration factor for Cu which could be, at least partly, responsible for its sensitivity to this toxic element. The impact of stress on K and Ca homeostasis is discussed in relation to ion distribution and presence of mucilage in the plant.

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Section: Discussionmentioning

confidence: 99%

Effects of Salinity on the Response of the Wetland Halophyte Kosteletzkya virginica (L.) Presl. to Copper Toxicity

Han

Lefèvre

Ruan

et al. 2011

Water Air Soil Pollut

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“…Previous studies have reported that, in various plant species, gene expression and function of PIPs can be modulated by heavy metals such as cadmium, copper, and mercury (Guo et al, 2006). Expression of PsPIP2-1 showed an increasing trend after HgCl 2 [mercury (II) chloride] treatment in Pisum sativum (Beaudette et al, 2007), whereas copper stress genes encoding plasmalemma PIP were down-regulated under copper application in Populus deltoides roots (Guerra et al, 2009). Similarly, 15 tags annotated to PIPs were significantly down-regulated, while two tags annotated to Arabidopsis PIPs were up-regulated in response to cadmium stress in Solanum torvum.…”

Section: Deps Between Pb1000-grown and Control Groupsmentioning

confidence: 98%

Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics

Gao

Peng

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Lead (Pb), a heavy metal, has become a crucial pollutant in soil and water, causing not only permanent and irreversible health problems, but also substantial reduction in crop yields. In this study, we conducted proteome analysis of the roots of the non-hyperaccumulator inbred maize line 9782 at four developmental stages (0, 12, 24, and 48 h) under Pb pollution using isobaric tags for relative and absolute quantification technology. A total of 252, 72 and 116 proteins were differentially expressed between M12 (after 12-h Pb treatment) and CK (water-mocked treatment), M24 (after 24-h Pb treatment) and CK, and M48 (after 48-h Pb treatment) and CK, respectively. In addition, 14 differentially expressed proteins were common within each comparison group. Moreover, Cluster of Orthologous Groups enrichment analysis revealed predominance of the proteins involved in posttranslational modification, protein turnover, and chaperones. Additionally, the changes in protein profiles showed a lower concordance with corresponding alterations in transcript levels, indicating important roles for transcriptional and posttranscriptional regulation in the response of maize roots to Pb pollution. Furthermore, enriched functional categories between the successive comparisons showed that the proteins in functional categories of stress, redox, signaling, and transport were highly up-regulated, while those in the functional categories of nucleotide metabolism, amino acid metabolism, RNA, and protein metabolism were down-regulated. This information will help in furthering our understanding of the detailed mechanisms of plant responses to heavy metal stress by combining protein and mRNA profiles.

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“…Borghi et al (2007) found that the Cu concentration in the leaves and stems was only about 10 μg/g DW, while the root Cu concentration reached 2000-4000 μg/g DW; similarly, Borghi et al (2008) noted that the average Cu concentration was 5.9-8.8 μg/g DW in the leaves and stems, whereas the root Cu concentration reached 6×10 5 -1.4×10 6 μg/g DW. Guerra et al (2009) found the Cu concentration in the leaves of Populus deltoides to be close to 50 μg/g DW, while the Cu concentration in the roots ranged from 5000 to 6000 μg/g DW. Indeed, these studies on poplar using the hydroponic system evaluated Cu toxicity tolerance rather than Cu accumulation potential.…”

Section: Variation In Cu and Zn Accumulationmentioning

confidence: 99%

Variation in copper and zinc tolerance and accumulation in 12 willow clones: implications for phytoextraction

Yang

Wang

Zhao

et al. 2014

J. Zhejiang Univ. Sci. B

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Willows (Salix spp.) have shown high potential for the phytoextraction of heavy metals. This study compares variations in copper (Cu) and zinc (Zn) tolerance and accumulation potential among 12 willow clones grown in a nutrient solution treated with 50 μmol/L of Cu or Zn, respectively. The results showed differences in the tolerance and accumulation of Cu and Zn with respect to different species/clones. The biomass variation among clones in response to Cu or Zn exposure ranged from the stimulation of growth to inhibition, and all of the clones tested showed higher tolerance to Cu than to Zn. The clones exhibited less variation in Cu accumulation but larger variation in Zn accumulation. Based on translocation factors, it was found that most of the Cu was retained in the roots and that Zn was more mobile than Cu for all clones. It is concluded that most willow clones are good accumulators of Zn and Cu.

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Gene expression analysis of Populus deltoides roots subjected to copper stress

Cited by 31 publications

References 54 publications

Effects of Salinity on the Response of the Wetland Halophyte Kosteletzkya virginica (L.) Presl. to Copper Toxicity

Effects of Salinity on the Response of the Wetland Halophyte Kosteletzkya virginica (L.) Presl. to Copper Toxicity

Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics

Variation in copper and zinc tolerance and accumulation in 12 willow clones: implications for phytoextraction

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