Gene expression and activity of antioxidant enzymes in barley (<i>Hordeum vulgare</i>L.) under controlled severe drought

Harb, Amal; Awad, Dalal; Samarah, Nezar H.

doi:10.1080/17429145.2015.1033023

Cited by 88 publications

(67 citation statements)

References 27 publications

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“…On the other hand, inconsistent expression profile of SOD, CAT and POX genes with the corresponding enzyme activity under 25mM salt stress conditions was also recorded. In accordance with other researchers (Gill &Tuteja, 2010 andHarb et al, 2015), this finding could be attributed to the presence of isoforms of the antioxidant enzymes. At the biochemical level, the activities of all isoforms were assayed, whereas at the transcriptional level, the expression of only one isoform was examined (Furlan et al, 2013).…”

Section: Discussionsupporting

confidence: 93%

Exogenous Salicylic acid Ameliorates the Adverse Effects of Salt Stress on Antioxidant System in Rosmarinus officinalis L.

2018

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S ALT stress is a main factor limiting plant growth and productivity. Salicylic acid (SA) has been shown to alleviate the adverse effects of different environmental stresses on plants. To investigate the protective role of salicylic acid (SA) in alleviating salt stress on Rosmarinus officinalis L. (rosemary) plant, a pot experiment was conducted to measure the growth parameters such as plant height and branch number. An antioxidant defense system, represented by catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX), was evaluated at both biochemical and transcriptional levels. NaCl was applied at 0, 25, 50 and 100mM and 0, 0.2 and 0.4mM of SA were used. The exposure of rosemary plant to salt conditions resulted in significant reduction of plant height, branches number and increases in the three measured antioxidant enzyme activities, compared to control. The foliar application of SA effectively increased growth rates and enhanced the activities of CAT, SOD and POX enzymes. The PCR (RT-PCR) analysis revealed that the relative transcript levels of SOD, CAT, and POX genes were changed and up-regulated compared to the control due to NaCl stress and SA treatments, reached the highest expression level by applying 25mM NaCl and 0 SA treatments. The results implied that SA regulated the transcript levels of the antioxidant genes, resulting in the increased contents of antioxidant enzymes and enhanced salt tolerance.

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Section: Discussionsupporting

confidence: 93%

Exogenous Salicylic acid Ameliorates the Adverse Effects of Salt Stress on Antioxidant System in Rosmarinus officinalis L.

2018

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“…The STBd line accumulated the lowest amount of MDA probably due to the rapid expression of the SOD, CAT, and POX antioxidant enzymes. Such antioxidant enzymes are considered as reliable indicators of environmental stress tolerance in plants (Harb et al 2015). These data correlate with those of Scandalios (1993), who reported that the efficient protection against oxidative damage caused by salt stress results from high SOD and CAT activities.…”

Section: Discussionsupporting

confidence: 69%

Enhanced antioxidant enzyme activities and respective gene expressionsin potato somatic hybrids under NaCl stress

Jbir-Koubaa¹,

Charfeddine²,

Bouaziz³

et al. 2019

Biologia plant.

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Potato (Solanum tuberosum L.), a plant of great economic importance worldwide, is known to be highly sensitive to salinity. Improving the tolerance of this crop was envisaged using interspecific somatic hybridization. In this report, the impact of salinity on three hybrid lines (STBa, STBc, and STBd) produced by protoplast fusion between the cv. BF15 and the wild species Solanum berthaultii was investigated in vitro. An analysis of plant response to oxidative stress was considered when plantlets were submitted to 100 mM NaCl for 5 d. The peroxidation of membrane lipids was screened by measuring malondialdehyde accumulation in these lines. Moreover, gene expressions and activities of antioxidant enzymes, such as catalase (CAT), peroxidase (POX), and superoxide dismutase (SOD), were assessed. The results show a lower degree of lipid peroxidation in the hybrid lines in comparison to the BF15 parent. These hybrids also showed higher activities of CAT, POX, and SOD than the BF15, especially in roots. The significant inductions of FeSOD, (Cu-Zn)SOD, MnSOD, and CAT genes in hybrid plants suggest their participation in salt tolerance. The differential expressions of the SOD and CAT genes between leaves and roots also indicate their tissue specificity.

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“…Moreover, it was recently reported that BRs induce apoplastic H 2 O 2 accumulation (Nie et al , Xia et al ), therefore analysis of an impact of BRs on the non‐enzymatic antioxidant system upon application of exogenous BR may become difficult to interpret because of this secondary H 2 O 2 ‐mediated effect, and also because the BR‐induced accumulation of H 2 O 2 results in stimulation of ABA biosynthesis (Zhou et al ). Therefore, the role of endogenous BRs in the regulation of non‐enzymatic antioxidant homeostasis under the control and drought conditions remains not fully elucidated, especially in crop species (Harb et al , Kka et al ).…”

Section: Discussionmentioning

confidence: 99%

Non‐enzymatic antioxidant accumulations in BR‐deficient and BR‐insensitive barley mutants under control and drought conditions

Gruszka

Janeczko

Dziurka

et al. 2018

Physiologia Plantarum

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Drought is one of the most adverse stresses that affect plant growth and yield. Disturbances in metabolic activity resulting from drought cause overproduction of reactive oxygen species. It is postulated that brassinosteroids (BRs) regulate plant tolerance to the stress conditions, but the underlying mechanisms remain largely unknown. An involvement of endogenous BRs in regulation of the antioxidant homeostasis is not fully clarified either. Therefore, the aim of this study was to elucidate the role of endogenous BRs in regulation of non-enzymatic antioxidants in barley (Hordeum vulgare) under control and drought conditions. The plant material included the 'Bowman' cultivar and a group of semi-dwarf near-isogenic lines (NILs), representing mutants deficient in BR biosynthesis or signaling. In general, accumulations of 11 compounds representing various types of non-enzymatic antioxidants were analyzed under both conditions. The analyses of accumulations of reduced and oxidized forms of ascorbate indicated that the BR mutants contain significantly higher contents of dehydroascorbic acid under drought conditions when compared with the 'Bowman' cultivar. The analysis of glutathione accumulation indicated that under the control conditions the BR-insensitive NILs contained significantly lower concentrations of this antioxidant when compared with the rest of genotypes. Therefore, we postulate that BR sensitivity is required for normal accumulation of glutathione. A complete accumulation profile of various tocopherols indicated that functional BR biosynthesis and signaling are required for their normal accumulation under both conditions. Results of this study provided an insight into the role of endogenous BRs in regulation of the non-enzymatic antioxidant homeostasis.

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Gene expression and activity of antioxidant enzymes in barley (Hordeum vulgareL.) under controlled severe drought

Cited by 88 publications

References 27 publications

Exogenous Salicylic acid Ameliorates the Adverse Effects of Salt Stress on Antioxidant System in Rosmarinus officinalis L.

Exogenous Salicylic acid Ameliorates the Adverse Effects of Salt Stress on Antioxidant System in Rosmarinus officinalis L.

Enhanced antioxidant enzyme activities and respective gene expressionsin potato somatic hybrids under NaCl stress

Non‐enzymatic antioxidant accumulations in BR‐deficient and BR‐insensitive barley mutants under control and drought conditions

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