Gene expression and in vitro replication of bovine gammaherpesvirus type 4

Romeo, Florencia; Uriarte, Enrique Leopoldo Louge; González-Altamiranda, Erika; Delgado, Santiago; Pereyra, Susana; Morán, Pedro Edgardo; Odeón, Anselmo; Pérez, Sandra; Verna, Andrea Elizabeth

doi:10.1007/s00705-020-04898-8

Cited by 4 publications

(8 citation statements)

References 40 publications

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“…A mechanism involved in the limitation or blocking of virion neutralization by antibodies seems to be the N-and O-glycans of the BoGHV4 gp180, which masks antigenic epitopes in gB, gH, and gL [10]. Our research group showed that isolate 07-435 induced the production of high levels of neutralizing antibodies in different ruminant species, including cattle, deer, sheep, and goat [23][24][25]. On the contrary, in the same studies, the absence of neutralizing antibody response was observed for isolate 10-154.…”

Section: Discussionmentioning

confidence: 89%

“…Supporting this, Dubuisson et al [26] demonstrated in their research that the humoral immune response in bovines infected with BoGHV4 is characterized by low efficiency or low levels of neutralizing antibodies. Therefore, this finding underscores antigenic differences in strain 07-435 that are capable of inducing a neutralizing humoral response in the host [24,25].…”

Section: Introductionmentioning

confidence: 77%

“…The high genomic divergence between the Argentinean and the available BoGHV4 CG as well as the body of research showing differences in biological features between isolates 07-435 and 10-154 [23][24][25]35,36] motivated the detailed analysis of different ORFs. In accordance with the CG divergency of both isolates, variations in nt and aa were also observed in the 10 further explored ORFs.…”

Section: Discussionmentioning

confidence: 99%

“…Previous research conducted by our group revealed marked differences in molecular and biological features between 07-435 and 10-154 strains, including in vitro and in vivo replication, gene expression, antibody neutralization, and protein profile [23][24][25]35,36]. These findings prompted further characterization of the ORFs encoding IE2, gH, gL, gM, gB, gp180, and Bo17 genes.…”

Section: Orf Analyses 241 Sequence Analysesmentioning

confidence: 94%

“…Verna et al [18] demonstrated a high genetic variability of Argentinean BoGHV4 strains isolated from vaginal fluids of aborted cows and the existence of a new phylogenetic group known as Genotype 3. Romeo et al [23,24] and Verna et al [25] conducted comparative studies of two Argentinean BoGHV4 strains corresponding to Genotypes 2 and 3 (10-154 and 07-435 strains, respectively), showing significant molecular and biological distinctions between these isolates. A noteworthy finding is the feature of strain 07-435 to be neutralized by sera from various ruminant species [23], highlighting a distinctive trait of this strain.…”

Section: Introductionmentioning

confidence: 99%

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Whole Genome Sequence-Based Analysis of Bovine Gammaherpesvirus 4 Isolated from Bovine Abortions

Romeo,

Spetter,

Pereyra

et al. 2024

Viruses

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Bovine gammaherpesvirus 4 (BoGHV4) is a member of the Gammaherspivirinae subfamily, Rhadinovirus genus. Its natural host is the bovine, and it is prevalent among the global cattle population. Although the complete genome of BoGHV4 has been successfully sequenced, the functions of most of its genes remain unknown. Currently, only six strains of BoGHV4, all belonging to Genotype 1, have been sequenced. This is the first report of the nearly complete genome of Argentinean BoGHV4 strains isolated from clinical cases of abortion, representing the first BoGHV4 Genotype 2 and 3 genomes described in the literature. Both Argentinean isolates presented the highest nt p-distance values, indicating a greater level of divergence. Overall, the considerable diversity observed in the complete genomes and open reading frames underscores the distinctiveness of both Argentinean isolates compared to the existing BoGHV4 genomes. These findings support previous studies that categorized the Argentinean BoGHV4 strains 07-435 and 10-154 as Genotypes 3 and 2, respectively. The inclusion of these sequences represents a significant expansion to the currently limited pool of BoGHV4 genomes while providing an important basis to increase the knowledge of local isolates.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Orf Analyses 241 Sequence Analysesmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Whole Genome Sequence-Based Analysis of Bovine Gammaherpesvirus 4 Isolated from Bovine Abortions

Romeo,

Spetter,

Pereyra

et al. 2024

Viruses

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First detection and impact of bovine herpesvirus type 4 on dairy cattle reproduction in Thailand

Jongsuwanwattana,

Setthawong,

Suadsong

et al. 2024

Vet World

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Background and Aim: Postpartum reproductive tract infections pose significant challenges to dairy farms, leading to economic losses due to reduced fertility associated with uterine inflammation. In veterinary practice, numerous research groups have explored the underlying causes of subfertility in cows, including surveying endemic viral infections related to endometritis in local areas. This study investigated bovine herpesvirus 4 (BoHV-4) infection in Thai dairy herds and assessed its impact on endometritis and subsequent reproductive outcomes. Materials and Methods: The present study analyzed BoHV-4 DNA in various samples, including milk, blood, and endometrial tissue, from 44 Holstein-Friesian cows 21–47 days postpartum across five dairy herds in Central Thailand. BoHV-4 glycoprotein B and thymidine kinase DNA sequences were detected using the polymerase chain reaction (PCR) and nested PCR, with sequence comparisons made to GenBank data for phylogenetic analysis. The endometritis status was diagnosed through vaginal mucus examination and endometrial cytology, with reproductive performance monitored up to the subsequent calving. Results: BoHV-4 DNA was identified in blood and endometrial tissues (15.91%) but not in milk samples. Phylogenetic analysis revealed that the local BoHV-4 strains are similar to those identified in Brazil and Japan. Notably, the presence of BoHV-4 was correlated with reduced postpartum reproductive performance, particularly extending the interval from calving to the first service. Conclusion: Our findings underscore the importance of integrating BoHV-4 genomic surveys and uterine health assessments to refine reproductive management strategies within the dairy industry. Keywords: bovine herpesvirus-4, dairy cattle, endometritis, phylogenetic analysis, reproductive performance, Thailand.

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Modulation of Apoptosis by Bovine Herpesvirus Type 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in this Process

Romeo,

Delgado,

Yavorsky

et al. 2023

Preprint

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The prevalent pathogens associated with bovine uterine infections are bacteria that appear to increase the host's susceptibility to secondary infections with other bacteria or viruses, among which BoHV-4 is the most frequently found. In this work, the study of the pathways of apoptosis induction was carried out on an experimental model of primary culture of endometrial cells, in order to know the implication of BoHV-4 and the presence of bacterial LPS in the pathogenesis of the bovine reproductive tract. For this, different staining techniques and molecular analysis by RT-PCR were used. The results obtained allowed us to conclude that the level of cell death observed in the proposed primary culture is directly related to the time of viral infection and the presence of LPS in BoHV-4 infection. The apoptosis indices in cells infected with BoHV-4, BoHV-4 + LPS presented a maximum that correlates with the appearance of the cytopathic effect, and the maximum viral titers in the model studied. However, morphological, biochemical and molecular changes were evident during both early and late stages of apoptosis. These findings provide information on the factors that may influence the pathogenesis of BoHV-4 and help to better understand the mechanisms involved in virus infection.

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Gene expression and in vitro replication of bovine gammaherpesvirus type 4

Cited by 4 publications

References 40 publications

Whole Genome Sequence-Based Analysis of Bovine Gammaherpesvirus 4 Isolated from Bovine Abortions

Whole Genome Sequence-Based Analysis of Bovine Gammaherpesvirus 4 Isolated from Bovine Abortions

First detection and impact of bovine herpesvirus type 4 on dairy cattle reproduction in Thailand

Modulation of Apoptosis by Bovine Herpesvirus Type 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in this Process

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