Gene expression and metabolite changes during Tuber magnatum fruiting body storage

Zampieri, Elisa; Guzzo, Flavia; Commisso, Mauro; Mello, Antonietta; Bonfante, Paola; Balestrini, Raffaella

doi:10.1007/s00294-014-0434-1

Cited by 8 publications

(6 citation statements)

References 31 publications

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“…Evolutionary analyses of genetic structures (Frizzi et al 2001 ), together with geographically specific ratios of climate-related stable isotopes, such as δ 15 N and δ 18 O (Krauß and Vetter 2020 ), of WTs help their market traceability from different regions (Bontempo et al 2020 ). This proved useful not only for protection against illegal trade and for preserving the volatile (enzyme) spectrum under different storage conditions but also for reconstructing dispersal gradients/pathways (Buzzini et al 2005 ; Pennazza et al 2013 ; Zampieri et al 2014 ; Vita et al 2015 ; Segelke et al 2020 ). Although still uncertain, the colonisation of new habitats may have been ongoing since the late Neogene, when the Apulo-Dalmatic Realm was formed (De Giuli et al 1987 ).…”

Section: Resultsmentioning

confidence: 99%

Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding

2023

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The white truffle (Tuber magnatum Picco.; WT) is the most expensive and arguably also the most delicious species within the genus Tuber. Due to its hidden belowground life cycle, complex host symbiosis, and yet unknown distribution, cultivation of the enigmatic species has only recently been achieved at some plantations in France. A sustainable production of WTs under future climate change, however, requires a better ecological understanding of the species’ natural occurrence. Here, we combine information from truffle hunters with a literature review to assess the climatic, edaphic, geographic, and symbiotic characteristics of 231 reported WT sites in southeast Europe. Our meta-study shows that 75% of the WT sites are located outside the species’ most famous harvest region, the Piedmont in northern Italy. Spanning a wide geographic range from ~ 37° N in Sicily to ~ 47° N in Hungary, and elevations between sea level in the north and 1000 m asl in the south, all WT sites are characterised by mean winter temperatures > 0.4 °C and summer precipitation totals of ~ 50 mm. Often formed during past flood or landslide events, current soil conditions of the WT sites exhibit pH levels between 6.4 and 8.7, high macroporosity, and a cation exchange capacity of ~ 17 meq/100 g. At least 26 potential host species from 12 genera were reported at the WT sites, with Populus alba and Quercus cerris accounting for 23.5% of all plant species. We expect our findings to contribute to a sustainable WT industry under changing environmental and economic conditions.

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Section: Resultsmentioning

confidence: 99%

Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding

2023

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“…Orthogonal projections to latent structures discriminant analysis (OPLS-DA) was then used to compare the infiltrated and non-infiltrated plants in terms of both primary ( Figures 8C,D ) and secondary ( Figures 9C,D ) metabolites. The S-loading plots revealed primary metabolites ( Figures 8E,F and Table 2 ) and secondary metabolites ( Figures 9E,F and Table 2 ) with pq(corr) values lower than -0.8 or higher than 0.8, indicating specific classes ( Zampieri et al, 2014 ). The imposition of three different classes (Δ87GAD65mut vs. agroinfiltrated control vs. wild-type) or two classes (Δ87GAD65mut vs. agroinfiltrated control, excluding wild-type) showed no statistically significant differences among the groups ( data not shown ).…”

Section: Resultsmentioning

confidence: 99%

Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen

et al. 2018

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Type-1 diabetes (T1D) is a metabolic disease involving the autoimmune destruction of insulin-producing pancreatic beta cells. It is often diagnosed by the detection of autoantibodies, typically those recognizing insulin itself or the 65-kDa isoform of glutamic acid decarboxylase (GAD65). Oral insulin can be used to induce systemic immunological tolerance and thus prevent or delay the onset of T1D, suggesting that combination treatments with other autoantigens such as GAD65 could be even more successful. GAD65 has induced oral tolerance and prevented T1D in preclinical studies but it is difficult to produce in sufficient quantities for clinical testing. Here we combined edible plant systems, namely spinach (Spinacia oleracea cv Industra) and red beet (Beta vulgaris cv Moulin Rouge), with the magnICON® expression system to develop a safe, cost-effective and environmentally sustainable platform for the large-scale production of GAD65. The superior red beet platform was extensively characterized in terms of recombinant protein yields and bioequivalence to wild-type plants, and the product was tested for its ability to resist simulated gastric digestion. Our results indicate that red beet plants are suitable for the production of a candidate oral vaccine based on GAD65 for the future preclinical and clinical testing of T1D immunotherapy approaches.

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“…Changes in linoleate-derived metabolites occurred during the first 3 days of cold storage, while the other metabolites, such as the amino acids, did not change. In order to have information on the molecular events occurring during refrigeration of T. magnatum, Zampieri et al (2014) also focused on the expression of genes involved in cell wall metabolism, stress tolerance and phenol oxidation, showing through RT-qPCR experiments that gene expression is altered during the storage period. A different time regulation was observed for ripe and less-ripe fruiting bodies suggesting that the considered genes are mostly expressed, under refrigeration, at an advanced maturation level.…”

Section: Monitoring the Impact Of Post-harvest Storage On The Qualitymentioning

confidence: 99%

Truffle Research in the Post-Genomics Era

Balestrini

Mello

2014

Food Anal. Methods

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The results originated in the frame of the genome sequencing project of the black truffle of Périgord (Tuber melanosporum) were published in 2010. Taking advantages from the sequencing project, which included automatic and manual annotation steps, specific studies were developed, in order to focus on specific gene categories (e.g. cell wallrelated genes, carbohydrate metabolism, environmental response genes, etc.) and, among them, specific gene families. This has allowed us to have, together with the transcriptomic data obtained during the sequencing phase, new knowledge on metabolic processes that may happen during the complex life cycle of a symbiotic fungus, such as Tuber. The aim of this review is (i) to highlight the work that has been done in postgenomics stage to better understand the molecular mechanisms occurring during the interaction of this fungus with the host plant, as well as those involved in the formation of precious truffles and (ii) to describe the results derived from projects mainly devoted to truffle identification in processed products and quality monitoring, as well as to the contribution of bacteria to truffle aroma.

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Gene expression and metabolite changes during Tuber magnatum fruiting body storage

Cited by 8 publications

References 31 publications

Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding

Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding

Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen

Truffle Research in the Post-Genomics Era

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