Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages

Abstract

The present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catiguá at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture.

In silico and in vivo analysis of ABI3 and VAL2 genes during somatic embryogenesis of Coffea arabica: competence acquisition and developmental marker genes

“…Moreover, high expression of ABI3 at all times of embryogenic cell suspension in conjunction with somatic embryos regeneration showed the maintenance of high embryogenic competence over the period of time studied. Unlike other reports that suggest culture age decreases embryogenic competence (Ikeda-Iwai et al 2002;Raja et al 2009;Savita et al 2011;Torres et al 2015), our results show that the high proliferation rate of embryogenic cell suspension does not lead to embryogenic competence decrease because cell suspension lines with 7 months cultive presented the highest regeneration rates. In addition, the expression pattern of the gene CaABI3 could be even higher for the ECS in every analysed times (Fig.…”

“…Distinction between embryogenic and nonembryogenic callus is important factor to improve protocols for induction and regeneration of somatic embryos in vitro (Ribas et al 2011;Silva et al 2014Silva et al , 2015Bartos et al 2018). Embryogenic cell suspensions can simultaneously present proembryogenic and non-embryogenic masses (Mazarei et al 2011;Torres et al 2015), as it was also observed in both lines evaluated in our study at all cultivation times. It is believed that this type of heterogeneity material can affect cell suspensions viability, since non-embryogenic cells have higher rates of cell division and, therefore, faster growth (Ribas et al 2011).…”

“…The somatic embryogenesis induction is related to biochemical and physiological modifications (Abbasi et al 2016), with changes in the explants gene expression pattern and in the competent cells reprogramming that will be involved in the embryogenic process (Karami et al 2009). Among the genes characterized in Coffea sp., emphasis has been given to the expression of SERK (Somatic Embryogenesis Receptor-Like Kinase), BBM (Baby Boom), WOX4 (Wuschel-Related Homeobox4) and LEC1 (LEAFY COT-YLEDON1) genes (Nic-Can et al 2013;Silva et al 2014Silva et al , 2015Torres et al 2015). Most of our knowledge about molecular regulation during somatic embryogenesis has been derived from others species such as Arabidopsis, while our knowledge about mechanisms that promote gene expression regulation of embryo development in coffee is limited.…”

Histological Analyses Reveal Promising Features in Coffea arabica Cell Suspension Culture

“…These characteristics corroborate with those observations in the cell suspensions of 10 months of this study. In another study, embryogenic cell suspensions of C. arabica cultivar Catiguá MG2 showed embryogenic characteristics as described in the present work with embryogenic cell suspensions cultivar Yellow Bourbon [31].…”

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

“…Microarray experiments revealed that BBM regulates a set of genes involved in growth and cell proliferation (Passarinho et al ). SERK and BBM displayed similar expression patterns in cell suspension cultures of C. arabica , indicating a close correlation in activity (Torres et al ). In Larix decidua Mill., SERK1 and BBM showed similar expression patterns during zygotic and somatic embryogenesis, while LEC1 exhibited an inverse expression pattern (Rupps et al ).…”