Gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses

Suekawa, Marina; Fujikawa, Yukichi; Inada, Shuhei; Murano, Asako; Esaka, Muneharu

doi:10.1016/j.jplph.2016.05.015

Cited by 21 publications

(14 citation statements)

References 42 publications

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“…The absorbance was measured at 340 nm using a Tecan Infinite M200 PRO microplate reader. The reaction was initiated according to a previous method ( Suekawa et al, 2016 ) by the addition of 20 μL of protein extract to a reaction mixture containing 50 mM Tris-HCl (pH 7.2), 0.1 mM NADPH, and 2 mM MG in a total volume of 200 μL. The absorbance was monitored for 2 min.…”

Section: Methodsmentioning

confidence: 99%

Silencing of D-Lactate Dehydrogenase Impedes Glyoxalase System and Leads to Methylglyoxal Accumulation and Growth Inhibition in Rice

Lan

Deng

et al. 2017

Front. Plant Sci.

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D-Lactate is oxidized by two classes of D-lactate dehydrogenase (D-LDH), namely, NAD-dependent and NAD-independent D-LDHs. Little is known about the characteristics and biological functions of D-LDHs in rice. In this study, a functional NAD-independent D-LDH (LOC_Os07g06890) was identified in rice, as a result of alternative splicing events. Characterization of the expression profile, subcellular localization, and enzymatic properties of the functional OsD-LDH revealed that it is a mitochondrial cytochrome-c-dependent D-LDH with high affinity and catalytic efficiency. Functional analysis of OsD-LDH RNAi transgenic rice demonstrated that OsD-LDH participates in methylglyoxal metabolism by affecting the activity of the glyoxalase system and aldo-keto reductases. Under methylglyoxal treatment, silencing of OsD-LDH in rice resulted in the accumulation of methylglyoxal and D-lactate, the decrease of reduced glutathione in leaves, and ultimately severe growth inhibition. Moreover, the detached leaves of OsD-LDH RNAi plants were more sensitive to salt stress. However, the silencing of OsD-LDH did not affect the growth under photorespiration conditions. Our results provide new insights into the role of NAD-independent D-LDHs in rice.

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Section: Methodsmentioning

confidence: 99%

Silencing of D-Lactate Dehydrogenase Impedes Glyoxalase System and Leads to Methylglyoxal Accumulation and Growth Inhibition in Rice

Lan

Deng

et al. 2017

Front. Plant Sci.

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“…For example, overexpression of AER stimulates salt tolerance in Arabidopsis (Papdi et al, 2008). Salt stress also enhanced the AKR4B gene expressions in tomato (Suekawa et al, 2016) and AKR1-overexpressing tobacco plants showed enhanced antioxidant capacity (Vemanna et al, 2017). Yalcinkaya et al (2019a) compared the response of the redox regulatory system in glycophytic A. thaliana and halophytic S. parvulum to exogenously applied RCS and found that the H 2 O 2 scavenging enzymes of S. parvulum were not affected as much as of A. thaliana by RCS treatments.…”

Section: Ros Metabolism Under Salt Stressmentioning

confidence: 99%

Plant response to salinity: an analysis of ROS formation, signaling, and antioxidant defense

Akyol¹,

Yilmaz²,

Uzilday³

et al. 2020

Turk J Bot

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Reactive oxygen species (ROS) are byproducts of normal plant metabolism and their production is elevated under environmental stresses such as drought, extreme temperature, and salinity. Among these, salinity is a worldwide problem that impacts the fertility of arable lands and sustainability of food security, which is getting more attention due to climate change. Halophytes can survive and reproduce in soils containing high concentrations of salt and have developed adaptation mechanisms at physiological, biochemical, and molecular levels including maintenance of ROS metabolism. In this review, we aim to summarize findings related to ROS production, signaling, scavenging, and especially ROS avoidance mechanisms under salt stress. In addition, expressions of antioxidant genes in Arabidopsis thaliana and its close relative, the model halophyte Schrenkiella parvula, are compared. Moreover, time-course expression levels of genes encoding major antioxidant enzymes in the model plant A. thaliana are analyzed with publicly available data to understand rapid responses of antioxidant defense under salt stress. The role of ROS-Ca +2 interaction and involvement of NADPH oxidases in this process are also discussed in the context of the perception and signaling of salt stress.

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“…Were GalUR genes a subgroup of AKR genes? According to previous reports, not all GalUR homologs contributed to the AsA content in plants, and it appears that the role of GalUR proteins in AsA biosynthesis might be unique [12]. Do the neofunctionalization or subfunctionalization models apply to these genes?…”

Section: Introductionmentioning

confidence: 99%

“…All GalUR protein sequences that were analyzed shared significant homology with cytosolic NAD(P)H-dependent aldo-keto reductase (AKR) with the specific domain Aldo-ket_red (PF00248) [12]. AKR comprises a diverse family of enzymes that catalyze the reduction of carbonyl compounds to the corresponding alcohols (or engage in reverse oxidation) and are widely present in fungi, animals, and plants [13].…”

Section: Introductionmentioning

confidence: 99%

Molecular Evolutionary and Expression Pattern Analysis of AKR Genes Shed New Light on GalUR Functional Characteristics in Brassica rapa

Duan

Huang

Liu

et al. 2020

IJMS

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The aldo-keto reductase (AKR) superfamily plays a major role in oxidation-reduction in plants. D-galacturonic acid reductase (GalUR), an ascorbic acid (AsA) biosynthetic enzyme, belongs to this superfamily. However, the phylogenetic relationship and evolutionary history of the AKR gene family in plants has not yet been clarified. In this study, a total of 1268 AKR genes identified in 36 plant species were used to determine this phylogenetic relationship. The retention, structural characteristics, and expression patterns of AKR homologous genes in Brassica rapa and Arabidopsis thaliana were analyzed to further explore their evolutionary history. We found that the AKRs originated in algae and could be divided into A and B groups according to the bootstrap value; GalURs belonged to group A. Group A AKR genes expanded significantly before the origin of angiosperms. Two groups of AKR genes demonstrated functional divergence due to environmental adaptability, while group A genes were more conservative than those in group B. All 12 candidate GalUR genes were cloned, and their expression patterns under stress were analyzed, in Pak-choi. These genes showed an obvious expression divergence under multiple stresses, and BrcAKR22 exhibited a positive correlation between its expression trend and AsA content. Our findings provide new insights into the evolution of the AKR superfamily and help build a foundation for further investigations of GalUR’s functional characteristics.

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Gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses

Cited by 21 publications

References 42 publications

Silencing of D-Lactate Dehydrogenase Impedes Glyoxalase System and Leads to Methylglyoxal Accumulation and Growth Inhibition in Rice

Silencing of D-Lactate Dehydrogenase Impedes Glyoxalase System and Leads to Methylglyoxal Accumulation and Growth Inhibition in Rice

Plant response to salinity: an analysis of ROS formation, signaling, and antioxidant defense

Molecular Evolutionary and Expression Pattern Analysis of AKR Genes Shed New Light on GalUR Functional Characteristics in Brassica rapa

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