Gene expression and protein secretion patterns in decidual macrophages and different M1 and M2 macrophage populations with focus on M-CSF and IL-10 as polarising factors

Svensson, J.; Jenmalm, Maria C.; Matussek, Andreas; Geffers, Robert; Berg, Göran; Ernerudh, Jan

doi:10.1016/j.jri.2011.06.038

Cited by 1 publication

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“…We supposed that the upregulated Alistipes stimulated the intestine to secrete TLR4, which could induce the macrophages into proinflammatory M1 phenotype via activating the NF-κB , to affect the dynamics of bone resorption and formation . As displayed by ELISA, the M-CSF content in OVX, which could induce the macrophage differentiation, , was significantly increased compared to those in ACP. In addition, the immunohistochemical staining shows that the CD68 content of the duodenum in ACP was remarkably lower than OVX ( P < 0.001).…”

Section: Discussionmentioning

confidence: 99%

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Arecanut (Areca catechu L.) Seed Polyphenol-Ameliorated Osteoporosis by Altering Gut Microbiome via LYZ and the Immune System in Estrogen-Deficient Rats

Mei

Meng

et al. 2020

J. Agric. Food Chem.

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Polyphenol can improve osteoporosis and is closely associated with gut microbiota, while the mechanism and the relationship among polyphenol, osteoporosis, and gut microbiota colonization remain unclear. Here, an osteoporosis rat model established by ovariectomy was employed to investigate the improving mechanism of arecanut (Areca catechu L.) seed polyphenol (ACP) on osteoporosis by regulating gut microbiota. We analyzed the bone microstructure, Paneth cells, regulating microbial protein (lysozyme (LYZ)), proinflammatory cytokines, macrophage infiltration levels, and gut microbial communities in a rat. ACP improved the trabecular microstructure compared to OVX, including the increased trabecular number (Tb.N) (P < 0.01) and trabecular thickness (Tb.Th) (P < 0.001) and decreased trabecular separation (Tb.Sp) (P < 0.01). At the phylum level, Bacteroidetes was increased after ovariectomy (P < 0.001) and Firmicutes and Proteobacteria were increased in ACP (P < 0.001). Antiosteoporosis groups with lower LYZ and Paneth cells (P < 0.001) showed that the microbiota Alistipes, which have a negative effect on bone metabolism were decreased in ACP (P < 0.001). Altogether, these studies showed that the estrogen deficiency could induce the shedding of Paneth cells, which leads to the decrease of LYZ, while ACP could increase the LYZ expression by maintaining the population of Paneth cells in an estrogen-deficient host, which were implicated in gut microbiota regulation and improved osteoporosis by controlling the inflammatory reaction.

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Section: Discussionmentioning

confidence: 99%

“…It should be noted that the inflammatory IL-6, IL-8, and TNF-α, which were proven to have a negative effect on the bone health, , were maintained at a normal level compared with OVX. The bone resorption is induced by the innate immune response .…”

Section: Discussionmentioning

confidence: 99%

Arecanut (Areca catechu L.) Seed Polyphenol-Ameliorated Osteoporosis by Altering Gut Microbiome via LYZ and the Immune System in Estrogen-Deficient Rats

Mei

Meng

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

Gene expression and protein secretion patterns in decidual macrophages and different M1 and M2 macrophage populations with focus on M-CSF and IL-10 as polarising factors

Cited by 1 publication

References 0 publications

Arecanut (Areca catechu L.) Seed Polyphenol-Ameliorated Osteoporosis by Altering Gut Microbiome via LYZ and the Immune System in Estrogen-Deficient Rats

Arecanut (Areca catechu L.) Seed Polyphenol-Ameliorated Osteoporosis by Altering Gut Microbiome via LYZ and the Immune System in Estrogen-Deficient Rats

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