Gene expression changes in bioceramic paste-treated human dental pulp cells

Abstract

Abstract:We evaluated the gene expression profiles of human dental pulp cells exposed to iRoot BP using microarray after 24 and 72 h. The results were verified using quantitative reverse transcriptase PCR analysis. Of the 36,000 transcripts arrayed, 21 were up-regulated and 15 were down-regulated by more than two fold. The largest group of up-regulated genes included those involved in nucleobase-containing compound metabolic processes, cell communication, protein metabolic processes, developmental processes, and biological regulation. The largest groups of down-regulated genes were those involved in cell communication, development, and biological regulation processes. In conclusion, iRoot BP affects the expression of genes involved in different biological processes in human dental pulp cells. (J Oral Sci 58, 307-315, 2016)

Molecular mechanism of a novel root-end filling material containing zirconium oxide on the osteogenic/odontogenic differentiation of human osteosarcoma MG-63 cells

“…When combined with bioinformatics analysis, it allows for a comprehensive study of gene-gene relationships, making it possible to elucidate the molecular mechanisms underlying the different effects of biomaterials on tissues and cells. For instance, gene expression profiling microarray technology has been applied to investigate the molecular mechanisms of osteogenic differentiation in calcium silicate-based materials such as MTA and iRoot BP ( Kim et al, 2010 ; Torun et al, 2016 ). However, to date, there have been no comparative studies on the osteogenic/odontogenic differentiation mechanisms between calcium silicate-based materials (such as MTA) and hydroxyapatite-based materials (such as NRFM-Zr).…”