Gene expression changes of thermo‐sensitive transient receptor potential channels in obese mice

Sun, Wuping; Li, Chen; Zhang, Yonghong; Jiang, Changyu; Zhai, Mingzhu; Zhou, Qian; Xiao, Lizu; Deng, Qi

doi:10.1002/cbin.10783

Cited by 22 publications

(32 citation statements)

References 37 publications

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“…TRPV3 could be primarily activated by a high noxious threshold which is over 50°C and then becomes responsive to warm temperatures [66]. It has been reported that the expression level of TRPV3 was decreased in visceral adipose tissue in HFD-treatment mice, ob/ob and db/db mice [67], and also reduced in subcutaneous WAT and interscapular BAT in HFD-treated and db/db mice, which is similar to TRPV1 [68]. HFD feeding increases TRPV3 expression in the medial nucleus tractus solitarius (mNTS) and hypoglossal nucleus (HN) in rats, which is accompanied by a reduced expression of proopiomelanocortin (POMC) and resulted in increased food intake and a gain of body-weight [69].…”

Section: Trpv Family In Adipogenesismentioning

confidence: 85%

“…TRPV2 and TRPV4 function as an osmo-and/or mechano-sensor, which could be activated by hypotonic solution or mechanical stimulation [70][71][72][73]. TRPV2 has also been reported to be expressed in both WAT and BAT [68,74], which can be activated by noxious heat with the threshold above 52°C [75]. The expression level of TRPV2 is higher in mature adipocytes than in pre-adipocytes.…”

Section: Trpv Family In Adipogenesismentioning

confidence: 99%

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Involvement of calcium channels in the regulation of adipogenesis

Zhai

Yang

et al. 2020

Adipocyte

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As an important second messenger in adipocytes, calcium ions (Ca 2+) are essential in regulating various intracellular signalling pathways that control critical cellular functions. Calcium channels show selective permeability to Ca 2+ and facilitate Ca 2+ entry into the cytoplasm, which are normally located in the plasmatic and intracellular membranes. The increase of cytosolic Ca 2+ modulates a variety of signalling pathways and results in the transcription of target genes that contribute to adipogenesis, a key cellular event includes proliferation and differentiation of adipocyte. In the past decades, the involvement of some Ca 2+-permeable ion channels, such as Ca 2+ release-activated Ca 2+ channels, transient receptor potential channels, voltage-gated calcium channels and others, in adipogenesis has been extensively explored. In the present review, we provided a summary of the expression and contributions of these Ca 2+-permeable channels in mediating Ca 2+ influxes that drive adipogenesis. Moreover, we discussed their potentials as future therapeutic targets.

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Section: Trpv Family In Adipogenesismentioning

confidence: 85%

Section: Trpv Family In Adipogenesismentioning

confidence: 99%

Involvement of calcium channels in the regulation of adipogenesis

Zhai

Yang

et al. 2020

Adipocyte

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“…and Trpv3 mRNA were significantly decreased, whereas Trpv2 and Trpv4 mRNA were significantly increased in WAT of either db/db or diet-induced obesity mice [42]. It has been reported that TRPV1…”

Section: Discussionmentioning

confidence: 93%

A transcriptomic analysis reveals novel patterns of gene expression during 3T3-L1 adipocyte differentiation

Sun

Yang

et al. 2020

Preprint

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Background: Obesity is characterized by increased adipose tissue mass that results from increased fat cell size (hypertrophy) and number (hyperplasia). The molecular mechanisms that govern the regulation and differentiation of adipocytes play a critical role for better understanding of the pathological mechanism of obesity. However, the mechanism of adipocyte differentiation is still unclear. Objective: The present study aims to compare the gene expression changes during adipocyte differentiation in the transcriptomic level, which may help to better understand the mechanism of adipocyte differentiation. Methods: RNA sequencing technology, GO and KEGG analysis and quantitative RT-PCR menthod were used in this study. Results: A lot of genes were up- or down- regulated between each two stages of 3T3-L1 adipocyte. GO and KEGG analysis revealed that lipid metabolism and oxidation-reduction reaction were mainly involved in the whole process of adipocyte differentiation. Moreover, decreased immune response and cell cycle, adhesion were occurred in the late phase of adipocyte differentiation, which were demonstrated by divergent expression pattern analysis. In addition, quantitative RT-PCR results demonstrated that the mRNA expression level of Trpv4 , Trpm4 , Trpm5 and Trpm7 were significantly decreased in the differentiated adipocytes. On the other hand, the mRNA expression level of Trpv1 , Trpv2 , Trpv6 and Trpc1 were significantly increased. Conclusions: These data presents the description of transcription profile changes in adipocyte differentiation and provides an in-depth analysis of the possible mechanisms of adipocyte differentiation. These data offer new insight into the understanding of the mechanisms of adipocyte differentiation.

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“…Recent research has also characterized the expression of TRPV1 in these tissues. TRPV1 expression has been shown on cultured adipocytes [90][91][92] and epididymal, subcutaneous and brown adipocytes [6,7,93]. The validation of expression of TRPV1 on adipose tissues suggests a plausible role of TRPV1 in the recruitment of BAT activity and thermogenesis and the induction of the molecular conversion of WAT to beige like cells.…”

Section: Adipose Depots Functions and Trpv1 Expressionmentioning

confidence: 87%

CAP and Metabolic Diseases: A Mini Review on Preclinical Mechanisms and Clinical Efficacy

Thyagarajan¹,

Krishnan²,

Baskaran³

2018

Capsaicin and Its Human Therapeutic Development

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Capsaicin (CAP) is the chief active ingredient of natural chili peppers. It has culinary and medicinal benefits. CAP activates its receptor, transient receptor potential vanilloid subfamily 1 (TRPV1), which is expressed in the sensory and motor neurons, adipocytes, liver, vascular smooth muscle cells, neuromuscular junction, skeletal muscle, heart and brain. The specificity of CAP to activate TRPV1 is the fundamental mechanism for its medicinal benefits to treat pain, obesity, hypertension, and other diseases. Preclinical data from rodent model of high fat diet-induced obesity collectively suggest that CAP exerts its effects by activating TRPV1 signaling pathway, which stimulates thermogenic mechanisms in the white and brown adipose tissues to induce browning of white adipose tissues and brown adipose tissue thermogenesis. This leads to enhancement of metabolic activity and thermogenesis to counter obesity. Although CAP and its pungent and nonpungent analogs are used in human clinical studies, their effects on satiety and energy expenditure have been the highlights of such studies. The precise mechanism of action of CAP has not been evaluated in humans. This article summarizes these data and suggests that long-term safety and tolerance studies are important for advancing CAP to treat human obesity.

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Gene expression changes of thermo‐sensitive transient receptor potential channels in obese mice

Cited by 22 publications

References 37 publications

Involvement of calcium channels in the regulation of adipogenesis

Involvement of calcium channels in the regulation of adipogenesis

A transcriptomic analysis reveals novel patterns of gene expression during 3T3-L1 adipocyte differentiation

CAP and Metabolic Diseases: A Mini Review on Preclinical Mechanisms and Clinical Efficacy

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