Gene Expression during Fracture Healing in Normal Mouse: In Situ Hybridization on Hard Tissues for Investigation of Regenerative Mechanisms.

Kitazawa, Riko; Kitazawa, Sohei

doi:10.1267/ahc.34.321

Cited by 6 publications

(5 citation statements)

References 47 publications

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“…5,12 BMP can induce differentiation of mesenchymal cells and stem cells into osteoblasts and chondroblasts, and can induce ectopic bone formation in soft tissue 7,13,14 . It has been demonstrated that BMP also plays an important role in embryogenesis 4 , and that BMP is expressed at bone fracture sites 8,9 . BMP chiefly induces new bone via the endochondral pathway.…”

mentioning

confidence: 99%

rh-BMP2-induced ectopic bone for grafting critical size defects: a preliminary histological evaluation in rat calvariae

Inoda

Yamamoto

Hattori

2007

International Journal of Oral and Maxillofacial Surgery

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confidence: 99%

rh-BMP2-induced ectopic bone for grafting critical size defects: a preliminary histological evaluation in rat calvariae

Inoda

Yamamoto

Hattori

2007

International Journal of Oral and Maxillofacial Surgery

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“…In contrast to that, in our study, both herbal extracts were not able to reverse osteogenic or chondrogenic potentials in the smoker in vitro fracture hematomas. Generally, it has to be remarked that for the analysis of osteogenic differentiation in fracture repair, longer incubation times would be preferable, since an increase in osteogenic differentiation potential is usually observed between 48 and 72 h post-fracture [65,66].…”

Section: Discussionmentioning

confidence: 99%

Herbal Extracts of Ginseng and Maqui Berry Show Only Minimal Effects on an In Vitro Model of Early Fracture Repair of Smokers

Rinderknecht,

Mayer,

Histing

et al. 2023

Foods

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Smoking is a major risk factor for delayed fracture healing, affecting several aspects of early fracture repair, including inflammation, osteogenesis, and angiogenesis. Panax ginseng (GE) and maqui berry extract (MBE) were shown in our previous studies to reduce smoke-induced cellular damage in late bone-healing in vitro models. We aimed here to analyze their effects on the early fracture repair of smokers in a 3D co-culture model of fracture hematomas and endothelial cells. Both extracts did not alter the cellular viability at concentrations of up to 100 µg/mL. In early fracture repair in vitro, they were unable to reduce smoking-induced inflammation and induce osteo- or chondrogenicity. Regarding angiogenesis, smoking-induced stress in HUVECs could not be counteracted by both extracts. Furthermore, smoking-impaired tube formation was not restored by GE but was harmed by MBE. However, GE promoted angiogenesis initiation under smoking conditions via the Angpt/Tie2 axis. To summarize, cigarette smoking strikingly affected early fracture healing processes in vitro, but herbal extracts at the applied doses had only a limited effect. Since both extracts were shown before to be very effective in later stages of fracture healing, our data suggest that their early use immediately after fracture does not appear to negatively impact later beneficial effects.

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“…Osteonectin is the most abundant noncollagen extracellular matrix protein in bone (33). Expression of Cbfa1, oseteonectin, and osteocalcin is observed in mesenchymal cells in bone fracture in mice for at least 21–28 days (3,16). Thus, expression of these factors for at least 21–28 days would be appropriate for effective bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed BMP-2 release from fibrin gel for 11 days (35). BMP-2 delivery for up to 21 days would be appropriate for effective bone formation, because BMP-2 expression is observed from day 2 to day 21 in bone fracture in mice (16). The results of a previous study demonstrated that BMP-2 released from scaffolds induced bone marrow-derived OPCs in peripheral blood to regenerate bone in the scaffolds (27).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Human Peripheral Blood Mononuclear Cell Transplantation-Mediated Bone Formation

Yang

Kim

et al. 2011

Cell Transplant

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Recent studies have demonstrated the existence of osteoblast progenitor cells in circulating blood. Here we show that local delivery of bone morphogenetic protein-2 (BMP-2) to cell transplantation sites induces in situ osteogenic differentiation of transplanted human peripheral blood mononuclear cells (PBMNCs) and enhances in vivo bone formation mediated by PBMNC transplantation. Human PBMNCs were seeded on scaffolds with or without BMP-2 and implanted subcutaneously into athymic mice. Nonseeded scaffolds with BMP-2 were also implanted. Eight weeks later, radiographic and histological analyses showed that the PBMNC + BMP-2 group had undergone much more extensive bone formation than either the PBMNC group or BMP-2 group. Only the PBMNC + BMP-2 group expressed human Cbfa1, osteonectin, and osteocalcin, suggesting in situ osteogenic differentiation of and bone formation by transplanted human PBMNCs, while the other groups did not express these genes. This study provides a method to enhance human PBMNC transplantation-mediated bone formation.

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Gene Expression during Fracture Healing in Normal Mouse: In Situ Hybridization on Hard Tissues for Investigation of Regenerative Mechanisms.

Cited by 6 publications

References 47 publications

rh-BMP2-induced ectopic bone for grafting critical size defects: a preliminary histological evaluation in rat calvariae

rh-BMP2-induced ectopic bone for grafting critical size defects: a preliminary histological evaluation in rat calvariae

Herbal Extracts of Ginseng and Maqui Berry Show Only Minimal Effects on an In Vitro Model of Early Fracture Repair of Smokers

Enhancement of Human Peripheral Blood Mononuclear Cell Transplantation-Mediated Bone Formation

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