Gene expression during the first 28 days of axolotl limb regeneration I: Experimental design and global analysis of gene expression

Voss, S. Randal; Palumbo, Alex; Nagarajan, Radha; Gardiner, David M.; Muneoka, Ken; Stromberg, Arnold J.; Athippozhy, Antony

doi:10.1002/reg2.37

Cited by 68 publications

(111 citation statements)

References 42 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…Indeed, Tara is also upregulated after pathogen-induced damage in the adult Drosophila gut (Chakrabarti et al, 2012). Furthermore, transcriptional profiling of regenerating tissue reveals the presence of TRIP-Br family members in the Xenopus tropicalis tadpole tail blastema and upregulation of TRIP-Br family members in regenerating zebrafish spinal cord and the axolotl limb blastema (Hui et al, 2014;Love et al, 2011;Voss et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Taranis Protects Regenerating Tissue from Fate Changes Induced by the Wound Response in Drosophila

Schuster

Smith-Bolton

2015

Developmental Cell

View full text Add to dashboard Cite

Regenerating tissue must replace lost structures with cells of the proper identity and function. How regenerating tissue establishes or maintains correct cell fates during regrowth is an open question. We have identified a gene, taranis, that is essential for maintaining proper cell fate in damaged and regenerating Drosophila wing imaginal discs but that is dispensable for these fates in normal wing development. In regenerating tissue with reduced levels of Taranis, expression of the posterior selector gene engrailed is silenced through an autoregulatory silencing mechanism that requires the PRC1 component polyhomeotic, resulting in a transformation of posterior tissue into anterior tissue late in regeneration. An essential component of the wound response, JNK signaling, induces this misregulation of engrailed expression. Taranis can suppress these JNK-induced cell fate changes without interfering with JNK signaling activity. Thus, taranis protects regenerating tissue from deleterious side effects of wound healing and regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Taranis Protects Regenerating Tissue from Fate Changes Induced by the Wound Response in Drosophila

Schuster

Smith-Bolton

2015

Developmental Cell

View full text Add to dashboard Cite

show abstract

“…Microarray and RNA‐Seq analysis of regenerating axolotl limbs has identified suites of genes encoding progenitor cell markers, stage‐specific genes, and genes regulated by neural signals (Knapp et al., 2013; Looso et al., 2013; Mercer et al., 2012; Monaghan et al., 2009, 2012; Stewart et al., 2013; Vascotto, Beug, Liversage, & Tsilfildis, 2005; Voss et al., 2015). Bryant, et al.…”

Section: Formation Of the Accumulation Blastemamentioning

confidence: 99%

“…Consistent with the scarring of denervated and amputated newt limbs, collagen fibrillogenesis begins prematurely (Bryant, Fyfe, & Singer, 1971; Vanrapenbush & Lasalle, 1989). The effect of denervation on a wide array of genes and proteins revealed by global genomic and proteomic analysis (Looso et al., 2013; Monaghan et al., 2009; Rao et al., 2009, 2014; Voss et al., 2015) is now wide open for investigation.…”

Section: Blastema Growthmentioning

confidence: 99%

Mechanisms of urodele limb regeneration

Stocum

2017

Regeneration

109

View full text Add to dashboard Cite

This review explores the historical and current state of our knowledge about urodele limb regeneration. Topics discussed are (1) blastema formation by the proteolytic histolysis of limb tissues to release resident stem cells and mononucleate cells that undergo dedifferentiation, cell cycle entry and accumulation under the apical epidermal cap. (2) The origin, phenotypic memory, and positional memory of blastema cells. (3) The role played by macrophages in the early events of regeneration. (4) The role of neural and AEC factors and interaction between blastema cells in mitosis and distalization. (5) Models of pattern formation based on the results of axial reversal experiments, experiments on the regeneration of half and double half limbs, and experiments using retinoic acid to alter positional identity of blastema cells. (6) Possible mechanisms of distalization during normal and intercalary regeneration. (7) Is pattern formation is a self‐organizing property of the blastema or dictated by chemical signals from adjacent tissues? (8) What is the future for regenerating a human limb?

show abstract

“…RA is present in regenerating limbs (Scadding and Maden, 1994) and RA-reporter axolotls show RA signaling in regenerating limbs (Monaghan and Maden, 2012b). Genes that regulate RA signaling are expressed in regenerating frog limbs (McEwan et al, 2011) and salamanders including Rdh10 (Monaghan et al, 2012), Raldh1 , Raldh3 (Monaghan et al, 2012), Rarα (Ragsdale et al, 1989) Rarβ (Carter et al, 2011;Giguere et al, 1989) and Rarγ (Hill et al, 1993;Ragsdale et al, 1989;Voss et al, 2015). Also, Raldh inhibition blocks axolotl limb regeneration (Maden, 1998;Scadding, 2000), and also epimorphic fin zebrafish regeneration (Blum and Begemann, 2012), and excess RA induces duplicated patterning during Xenopus hindlimb regeneration (Cuervo and ChimalMonroy, 2013) as it does in salamanders.…”

Section: Introductionmentioning

confidence: 99%

Retinoic acid receptor regulation of epimorphic and homeostatic regeneration in the axolotl

et al. 2017

Self Cite

View full text Add to dashboard Cite

Salamanders are capable of regenerating amputated limbs by generating a mass of lineage-restricted cells called a blastema. Blastemas only generate structures distal to their origin unless treated with retinoic acid (RA), which results in proximodistal (PD) limb duplications. Little is known about the transcriptional network that regulates PD duplication. In this study, we target specific retinoic acid receptors (RARs) to either PD duplicate (RA treatment or RARγ agonist) or truncate (RARβ antagonist) regenerating limbs. RARE-EGFP reporter axolotls showed divergent reporter activity in limbs undergoing PD duplication versus truncation, suggesting differences in patterning and skeletal regeneration. Transcriptomics identified expression patterns that explain PD duplication, including upregulation of proximal homeobox gene expression and silencing of distal-associated genes, whereas limb truncation was associated with disrupted skeletal differentiation. RARβ antagonism in uninjured limbs induced a loss of skeletal integrity leading to long bone regression and loss of skeletal turnover. Overall, mechanisms were identified that regulate the multifaceted roles of RARs in the salamander limb including regulation of skeletal patterning during epimorphic regeneration, skeletal tissue differentiation during regeneration, and homeostatic regeneration of intact limbs.

show abstract

Gene expression during the first 28 days of axolotl limb regeneration I: Experimental design and global analysis of gene expression

Cited by 68 publications

References 42 publications

Taranis Protects Regenerating Tissue from Fate Changes Induced by the Wound Response in Drosophila

Taranis Protects Regenerating Tissue from Fate Changes Induced by the Wound Response in Drosophila

Mechanisms of urodele limb regeneration

Retinoic acid receptor regulation of epimorphic and homeostatic regeneration in the axolotl

Contact Info

Product

Resources

About