Gene Expression during Vascular Pericyte Differentiation

Doherty, Mary Jo; Canfield, Ann E.

doi:10.1615/critreveukaryotgeneexpr.v9.i1.10

Cited by 110 publications

(76 citation statements)

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“…Moreover, in this model, we have described α-smooth muscle actin-positive (SMA+) cells with similar ultrastructural morphology to that of pericytes (Alliot-Licht et al 2001). Pericytes have been demonstrated to differentiate into osteoblasts (Brighton et al 1992;Diaz-Flores et al 1992;Schor et al 1995;Doherty et al 1998), and other studies have suggested that pericytes may also have the potential to differentiate into chondrocytes, adipocytes, smooth muscle cells, macrophages, and fibroblasts (Doherty and Canfield 1999;Farrington-Rock et al 2004). It is currently hypothesized that pericytes present in This work was supported by grants from the "Institut Français pour la Recherche Odontologique" (B. Alliot-Licht), INSERM EM 9903 (J. Guicheux), and University of Nantes (G. Bluteau, B. Lieubeau).…”

Section: Introductionsupporting

confidence: 52%

“…However, we cannot rule out the possibility that the decrease in the proportion of SMA+ cells is related to an indirect effect of Dex that induces changes in culture conditions, such as matrix deposition or growth factor synthesis. Indeed, these parameters are well-documented to influence the expression of SMA in culture (Shepro and Morel 1993;Verbeek et al 1994;Schor et al 1995;Doherty and Canfield 1999).…”

Section: Discussionmentioning

confidence: 99%

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Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures

et al. 2005

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Regenerative dental pulp strategies require the identification of precursors able to differentiate into odontoblast-like cells that secrete reparative dentin after injury. Pericytes have the ability to give rise to osteoblasts, chondrocytes, and adipocytes, a feature that has led to the suggestion that odontoblast-like cells could derive from these perivascular cells. In order to gain new insights into this hypothesis, we investigated the effects of dexamethasone (Dex), a synthetic glucocorticoid employed to induce osteogenic differentiation in vitro, in a previously reported model of human dental pulp cultures containing pericytes as identified by their expression of smooth muscle actin (SMA) and their specific ultrastructural morphology. Our data indicated that Dex (10(-8) M) significantly inhibited cell proliferation and markedly reduced the proportion of SMA-positive cells. Conversely, Dex strongly stimulated alkaline phosphatase (ALP) activity and induced the expression of the transcript encoding the major odontoblastic marker, dentin sialophosphoprotein. Nevertheless, parathyroid hormone/parathyroid hormone-related peptide receptor, core-binding factor a1/osf 2, osteonectin, and lipoprotein lipase mRNA levels were not modified by Dex treatment. Dex also increased the proportion of cells expressing STRO-1, a marker of multipotential mesenchymal progenitor cells. These observations indicate that glucocorticoids regulate the commitment of progenitors derived from dental pulp cells to form odontoblast-like cells, while reducing the proportion of SMA-positive cells. These results provide new perspectives in deciphering the cellular and molecular mechanisms leading to reparative dentinogenesis.

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Section: Introductionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures

et al. 2005

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“…Thus Brighton et al (1992), demonstrated that cultured bovine capillary and microvessel pericytes synthesized alkaline phosphatase and osteocalcin and formed colonies that mineralize their matrix. Studies by the group of Doherty and Canfield (Doherty and Canfield 1999;Doherty et al 1998) brought further strong evidence as they also showed that pericytes isolated from retinal capillaries express STRO-1, a primitive clonogenic marker of marrow stromal cells, and other markers of the osteoblast lineage, such as bone sialoprotein, osteocalcin, osteonectin, and osteopontin. They also inoculated the isolated pericytes into diffusion chambers that were implanted into athymic mice and achieved the formation of cartilage and bone nodules (Schor and Canfield 1998;Schor et al 1990).…”

Section: Discussionmentioning

confidence: 94%

Expression of vascular antigens by bone cells during bone regeneration in a membranous bone distraction system

Lewinson

Maor

Rozen

et al. 2001

Histochem Cell Biol

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An in vivo system of membranous bone formation during distraction has been investigated in order to follow cells that express vascular markers with the objective of understanding the neovascularization process. Concomitantly, sustained proliferation of preskeletal cells was achieved through the application of mechanical force. New capillaries and leading edges that arose by angiogenesis from the periosteal and mucosal surfaces and invaded the central zone of the regenerating distraction tissue temporally preceded the growth of delicate woven bone trabeculae from both edges of the cut bone. Concentrically arranged 'onion-like' configurations were abundant in paracentral zones and in association with mesenchymal condensations, suggesting their de novo formation in situ. Vascular specific markers, the angiopoietin receptor Tie-2 and factor VIII-related antigen (FVIIIrAg), were localized immunohistochemically in order to follow cells of vascular origin. Endothelial cells of the new capillaries, centrally located cells of the concentric configurations, pericytes, and most of the adjacent polygonal mesenchymal cells stained positively with specific antibodies to both antigens. Moreover, preosteoblasts and osteoblasts that lie adjacent to or already embedded in the osteiod of the newly formed trabeculae were also FVIIIrAg and Tie-2 immunopositive. As the source of the bone-forming cells in regenerating tissue during distraction is not yet fully understood, this observation might support the possibility of their vascular origin.

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“…It is also possible that pericytes constitute a cellular reserve for use in nonvessel tissue formation, remodeling, or repair, e.g., bone formation (Diaz-Flores et al 1992;Doherty and Canfield 2000). Pericytes have an intermediate phenotype between vSMC and fibroblasts and seem to have the capacity to differentiate also in the latter direction.…”

Section: General Characteristics and Functions Of Pericytesmentioning

confidence: 99%

Endothelial-pericyte interactions in angiogenesis

Gerhardt

Betsholtz

2003

Cell and Tissue Research

952

758

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It takes two to make blood vessels-endothelial cells and pericytes. While the endothelial cells are the better characterized of the two, pericytes are now coming into focus as important regulators of angiogenesis and blood vessel function, and as potential drug targets. However, pericytes are still surrounded by much controversy. They are difficult to define, they constitute a heterogeneous population of cells, and their ontogeny is not well understood. They are plastic and have the capacity to differentiate into other mesenchymal cell types, such as smooth muscle cells, fibroblasts and osteoblasts. Recent interest in pericytes also stems from their potential involvement in diseases such as diabetic microangiopathy, tissue fibrosis, cancer, atherosclerosis and Alzheimer's disease. The present review focuses on the role of pericytes in physiological angiogenesis. The currently favored view states that the initial endothelial tubes form without pericyte contact, and that subsequent acquisition of pericyte coverage leads to vessel remodeling, maturation and stabilization. Improved means of identifying and visualizing pericytes now challenge this view and show that high numbers of pericytes invest in actively sprouting and remodeling vessels. Genetic data demonstrate the critical importance of pericytes for vascular morphogenesis and function, and imply specific roles for the cell type in various aspects of angiogenesis.

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Gene Expression during Vascular Pericyte Differentiation

Cited by 110 publications

References 0 publications

Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures

Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures

Expression of vascular antigens by bone cells during bone regeneration in a membranous bone distraction system

Endothelial-pericyte interactions in angiogenesis

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