1989
DOI: 10.1016/0885-5765(89)90004-0
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Gene expression in Nicotiana tabacum in response to compatible and incompatible isolates of Pseudomonas solanacearum

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Cited by 32 publications
(22 citation statements)
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“…Total RNA extraction was done following either the guanidinium/cesium chloride method adapted by de Tapia et al (1987), or the phenol/chloroform/ isoamylalcohol technique (Ragueh et al 1989). Polyadenylated mRNAs were obtained by chromatographing twice total RNA on oligo-(dT) cellulose as described by Sambrook et al (1989).…”
Section: Methodsmentioning
confidence: 99%
“…Total RNA extraction was done following either the guanidinium/cesium chloride method adapted by de Tapia et al (1987), or the phenol/chloroform/ isoamylalcohol technique (Ragueh et al 1989). Polyadenylated mRNAs were obtained by chromatographing twice total RNA on oligo-(dT) cellulose as described by Sambrook et al (1989).…”
Section: Methodsmentioning
confidence: 99%
“…Preliminary experiments using the tobacco-P. solanacearum model system have shown that the HR-inducing isolate, GM11000, has an early and dramatic effect on plant gene expression. Significant changes in the mRNA population are not detected at this stage either with a H R -isolate, GMI1178 or with a compatible isolate, K60 [26]. Here, we describe the isolation of several cDNA clones corresponding to plant mRNAs whose expression has been studied in plants challenged with compatible and incompatible bacterial isolates.…”
Section: Introductionmentioning
confidence: 99%
“…Total RNA was isolated from 5 g of each sample according to the method described by Ragueh et al (1989). After grinding in liquid nitrogen, the material was resuspended in extraction buffer (200 mM Tris, pH 7.0, 20 mM EDTA, 100 mM NaCl, 1% SDS) and extracted twice with phenol:chloroform: isoamylalcohol (25:24:1).…”
Section: Rna Extraction and Purificationmentioning
confidence: 99%