Gene expression induction of volatile organic compound and/or polycyclic aromatic hydrocarbon-metabolizing enzymes in isolated human alveolar macrophages in response to airborne particulate matter (PM2.5)

Saint-Georges, Françoise; Abbas, Imane; Billet, Sylvain; Verdin, Anthony; Gosset, Pierre; Mulliez, P.; Shirali, Pirouz; Garçon, Guillaume

doi:10.1016/j.tox.2007.11.016

Cited by 45 publications

(30 citation statements)

References 45 publications

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“…A recent study has demonstrated that PAH coated onto PM 2.5 could induce gene expression of cyto-chrome P450 (CYP) 1A1, CYP2E1, NADPH quinone oxydo-reductase-1, and glutathione S -transferasepi 1 and mu 3 in human alveolar macrophages, suggesting the formation of biologically reactive metabolites and the role of carbonaceous core of PM as a physical carrier (Saint-Georges et al 2008). Quinones act as catalysts that produce ROS and may be key compounds in PM toxicity along with transition metals.…”

Section: Discussionmentioning

confidence: 99%

The Adjuvant Effect of Ambient Particulate Matter Is Closely Reflected by the Particulate Oxidant Potential

Li¹,

Wang

Bramble

et al. 2009

Environ Health Perspect

208

165

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BackgroundIt has been demonstrated that ambient particulate matter (PM) can act as an adjuvant for allergic sensitization. Redox-active organic chemicals on the particle surface play an important role in PM adverse health effects and may determine the adjuvant effect of different particle types according to their potential to perturb redox equilibrium in the immune system.ObjectivesWe determined whether the adjuvant effect of ambient fine particles versus ultrafine particles (UFPs) is correlated to their prooxidant potential.MethodsWe have established an intranasal sensitization model that uses ambient PM as a potential adjuvant for sensitization to ovalbumin (OVA), which enhances the capacity for secondary OVA challenge to induce allergic airway inflammation.ResultsUFPs with a greater polycyclic aromatic hydrocarbon (PAH) content and higher oxidant potential enhanced OVA sensitization more readily than did fine particles. This manifests as enhanced allergic inflammation upon secondary OVA challenge, leading to eosinophilic inflammation and mucoid hyperplasia starting at the nasal turbinates all the way down to the small pulmonary airways. The thiol antioxidant N-acetyl cysteine was able to suppress some of these sensitization events.ConclusionsThe adjuvant effects of ambient UFP is determined by their oxidant potential, which likely plays a role in changing the redox equilibrium in the mucosal immune system.

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Section: Discussionmentioning

confidence: 99%

The Adjuvant Effect of Ambient Particulate Matter Is Closely Reflected by the Particulate Oxidant Potential

Li¹,

Wang

Bramble

et al. 2009

Environ Health Perspect

208

165

View full text Add to dashboard Cite

show abstract

“…Organic chemical compounds such as oxy-PAHs and quinones are relevant organic chemical species in terms of PM redox chemistry and ROS generation (Bonvallot et al 2001;Cho et al 2005;Kumagai et al 1997;Monks et al 1992;Penning et al 1999). A recent study has demonstrated that PAH coated onto PM 2.5 could induce gene expression of cytochrome P450 (CYP) 1A1, CYP2E1, NADPH quinone oxydo-reductase-1, and glutathione S-transferase-pi 1 and mu 3 in human alveolar macrophages, suggesting the formation of biologically reactive metabolites and the role of carbonaceous core of PM as a physical carrier (Saint-Georges et al 2008). Quinones act as catalysts that produce ROS and may be key compounds in PM toxicity along with transition metals.…”

Section: Discussionmentioning

confidence: 99%

The Adjuvant Effect of Ambient Particulate Matter Is Closely Reflected by the Particulate Oxidant Potential

Li¹,

Wang²,

Bramble³

et al. 2009

Environ. Health Perspect.

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Background: It has been demonstrated that ambient particulate matter (PM) can act as an adjuvant for allergic sensitization. Redox-active organic chemicals on the particle surface play an important role in PM adverse health effects and may determine the adjuvant effect of different particle types according to their potential to perturb redox equilibrium in the immune system. oBjectives: We determined whether the adjuvant effect of ambient fine particles versus ultrafine particles (UFPs) is correlated to their prooxidant potential. Methods: We have established an intranasal sensitization model that uses ambient PM as a potential adjuvant for sensitization to ovalbumin (OVA), which enhances the capacity for secondary OVA challenge to induce allergic airway inflammation. results: UFPs with a greater polycyclic aromatic hydrocarbon (PAH) content and higher oxidant potential enhanced OVA sensitization more readily than did fine particles. This manifests as enhanced allergic inflammation upon secondary OVA challenge, leading to eosinophilic inflammation and mucoid hyperplasia starting at the nasal turbinates all the way down to the small pulmonary airways. The thiol antioxidant N-acetyl cysteine was able to suppress some of these sensitization events. conclusions: The adjuvant effects of ambient UFP is determined by their oxidant potential, which likely plays a role in changing the redox equilibrium in the mucosal immune system. key words: adjuvant, allergic inflammation, allergic sensitization, ambient ultrafine particles, asthma, oxidant potential, oxidative stress, redox-active organic chemicals, T H 2 immune response. Environ

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“…Increases in Cyp2e1 mRNA expression were observed in human alveolar epithelial carcinoma-derived cell line (A549) and in human alveolar macrophages incubated with fine particles (PM2.5) isolated from ambient air in the industrial area of the city of Dunkerque (France). It was suggested that volatile organic compounds (VOC; e.g., benzene, toluene, xylene), and polycyclic aromatic hydrocarbons (PAH; e.g., anthracene, phenanthrene, pyrene) both constituents of particulate air pollutants are responsible for the increased expression of Cyp2e1 in cell cultures (Billet et al 2007;Saint-Georges et al 2008). Cyp2e1 was also reported to be constitutively expressed in rat cardiomyocyte cultures, was found in all regions of human hearts and its expression was up-regulated in rat hearts exposed to benzo-α-pyrene (one of the PAHs identified in ambient PM) (Thum and Borlak 2000a;Thum and Borlak 2000b;Saint-Georges et al 2008;Aboutabl et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Once in the remote organ, volatile organic compounds (VOC) and PAH can up-regulate the expression of Cyp2e1 and undergo oxidative Phase I metabolism. Oxidation of VOC and PAH by the cytochrome P450 monooxygenase system could yield highly reactive epoxy compounds with increased toxic potentials (Billet et al 2007;Saint-Georges et al 2008). However, following phase I oxidation, activated foreign substances undergo Phase II reactions catalyzed primarily by glutathione-S-transferase.…”

Section: Discussionmentioning

confidence: 99%

Chronic exposure to ambient particulate matter alters cardiac gene expression patterns and markers of oxidative stress in rats

Simkhovich

Kleinman

Mehrian‐Shai

et al. 2010

Air Qual Atmos Health

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Exposure to ambient particulate matter (PM) significantly increases cardiovascular morbidity and mortality in the general population. We hypothesized that some components of PM can affect the gene expression patterns in the hearts of rats exposed for 3 months to filtered air (FA), coarse (CP; 2.51.5 (for both up-and down-regulated genes), we found three genes in the CP and nine genes in the UFP groups with significantly changed expression, compared with FA. No significant changes in gene expression patterns were observed in the FP group. In the UFP group thioredoxin interacting protein (Txnip), a negative regulator of an antioxidant enzyme thioredoxin, and cytochrome P450 (Cyp2e1), an enzyme involved in the metabolism of foreign substances demonstrated significant up-regulation (fold ratios 1.79 and 1.57, respectively, with false discovery rate, FDR<0.05). In the CP group there was also a trend towards increased Txnip expression (fold ratio 1.43, FDR>0.05) and significant increase in the Cyp2e1 expression (fold ratio 1.79, FDR<0.05). Changes in the Txnip and Cyp2e1 expression showed statistically significant positive correlation to each other (p<0.0009) and were confirmed by real-time PCR. In addition Txnip and Cyp2e1 expression demonstrated statistically significant moderate correlation with the levels of MDA in the heart. Upregulation of both Cyp2e1 and Txnip are observed in hearts of patients with certain cardiac diseases. Therefore, chronic exposure to CP and UFP directly affects expression of disease-relevant genes in the myocardium.

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Gene expression induction of volatile organic compound and/or polycyclic aromatic hydrocarbon-metabolizing enzymes in isolated human alveolar macrophages in response to airborne particulate matter (PM2.5)

Cited by 45 publications

References 45 publications

The Adjuvant Effect of Ambient Particulate Matter Is Closely Reflected by the Particulate Oxidant Potential

The Adjuvant Effect of Ambient Particulate Matter Is Closely Reflected by the Particulate Oxidant Potential

The Adjuvant Effect of Ambient Particulate Matter Is Closely Reflected by the Particulate Oxidant Potential

Chronic exposure to ambient particulate matter alters cardiac gene expression patterns and markers of oxidative stress in rats

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