Gene Expression Measurement Module (GEMM) for space application: Design and validation

Peyvan, Kianoosh; Karouia, Fathi; Cooper, John; Chamberlain, J S; Suciu, Dominic; Slota, Michael; Pohorille, Andrew

doi:10.1016/j.lssr.2019.07.004

Cited by 7 publications

(6 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracting products of interest at sufficient quality is equally essential to develop biomanufacturing. Systems such as Wetlab-2 or the Gene Expression Measurement Module (GEMM) illustrate the challenge of adapting biological sample processing for RNA extraction and molecular analysis in the microgravity environment 23,24 . In these systems, the sample mass and volume processed was small with the goal of providing biological inputs for analytical experiments 23,24 .…”

Section: Carbonicmentioning

confidence: 99%

“…Systems such as Wetlab-2 or the Gene Expression Measurement Module (GEMM) illustrate the challenge of adapting biological sample processing for RNA extraction and molecular analysis in the microgravity environment 23,24 . In these systems, the sample mass and volume processed was small with the goal of providing biological inputs for analytical experiments 23,24 . Future space biomanufacturing systems need to address post-growth processing at larger scales to extract products for in situ use.…”

Section: Carbonicmentioning

confidence: 99%

See 1 more Smart Citation

Theoretical design of a space bioprocessing system to produce recombinant proteins

Soundararajan

Paddock

Dougherty

et al. 2022

Preprint

View full text Add to dashboard Cite

Space-based biomanufacturing has the potential to improve the sustainability of deep space exploration. To advance biomanufacturing, bioprocessing systems need to be developed for space applications. Here, commercial technologies were assessed to design space bioprocessing systems to supply a liquid amine carbon dioxide scrubber with active carbonic anhydrase produced recombinantly. Design workflows encompassed biomass dewatering of 1 L Escherichia coli cultures through to recombinant protein purification. Equivalent system mass (ESM) analyses had limited utility for selecting specific technologies. Instead, bioprocessing system designs focused on minimizing complexity and enabling system versatility. Three designs that differed in biomass dewatering and protein purification approaches had nearly equivalent ESM of 357-522 kg eq. Values from the system complexity metric (SCM), technology readiness level (TRL), and degree of crew assistance metric identified a simpler, less costly, and easier to operate design for automated biomass dewatering, cell lysis, and protein affinity purification.

show abstract

Section: Carbonicmentioning

confidence: 99%

Section: Carbonicmentioning

confidence: 99%

Theoretical design of a space bioprocessing system to produce recombinant proteins

Soundararajan

Paddock

Dougherty

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The Key Considerations in Space Koehn [41] Discuss the design and prototype tests of the fast-imaging plasma spectrometer (FIPS) deflection system Lightweight, fast, and have a very large field of view Koga [42] Discuss the results of the engineering model (EM) and its properties Particle and plasma Krebs [43] Develop the mercury laser altimeter Space-flight environmental tests Lepri [44] Discuss an adaptation of the fast-imaging plasma spectrometer (FIPS) for the measurement of negatively charged particles. Design modification LIFE [45] Developed an automated, miniaturized, integrated fluidic system for in-situ measurements of gene expression in microbial samples…”

Section: Aims and Objectivesmentioning

confidence: 99%

“…A new neutron monitor instrument is designed to understand the particle acceleration mechanism at the solar surface. Life et al [45] design a new biological system that can be deployed in near future for space missions. platforms other than the ISS to advance biological research in space.…”

Section: Product Innovation and Designmentioning

confidence: 99%

A Systematic Review of Product Design for Space Instrument Innovation, Reliability, and Manufacturing

Yung

Tang

et al. 2021

Machines

View full text Add to dashboard Cite

The design and development of space instruments are considered to be distinct from that of other products. It is because the key considerations are vastly different from those that govern the use of products on planet earth. The service life of a space instrument, its use in extreme space environments, size, weight, cost, and the complexity of maintenance must all be considered. As a result, more innovative ideas and resource support are required to assist mankind in space exploration. This article reviews the impact of product design and innovation on the development of space instruments. Using a systematic literature search review and classification, we have identified over 129 papers and finally selected 48 major articles dealing with space instrument product innovation design. According to the studies, it is revealed that product design and functional performance is the main research focuses on the studied articles. The studies also highlighted various factors that affect space instrument manufacturing or fabrication, and that innovativeness is also the key in the design of space instruments. Lastly, the product design is important to affect the reliability of the space instrument. This review study provides important information and key considerations for the development of smart manufacturing technologies for space instruments in the future.

show abstract

“…Extracting products of interest at sufficient quality is equally essential to develop biomanufacturing. Systems such as Wetlab-2 or the Gene Expression Measurement Module (GEMM) illustrate the challenge of adapting biological sample processing for RNA extraction and molecular analysis in the microgravity environment 29 , 30 . In these systems, the sample mass and volume processed was small with the goal of providing biological inputs for analytical experiments 29 , 30 .…”

Section: Introductionmentioning

confidence: 99%

Theoretical design of a space bioprocessing system to produce recombinant proteins

Soundararajan,

Paddock,

Dougherty

et al. 2023

npj Microgravity

View full text Add to dashboard Cite

Space-based biomanufacturing has the potential to improve the sustainability of deep space exploration. To advance biomanufacturing, bioprocessing systems need to be developed for space applications. Here, commercial technologies were assessed to design space bioprocessing systems to supply a liquid amine carbon dioxide scrubber with active carbonic anhydrase produced recombinantly. Design workflows encompassed biomass dewatering of 1 L Escherichia coli cultures through to recombinant protein purification. Non-crew time equivalent system mass (ESM) analyses had limited utility for selecting specific technologies. Instead, bioprocessing system designs focused on minimizing complexity and enabling system versatility. Three designs that differed in biomass dewatering and protein purification approaches had nearly equivalent ESM of 357–522 kg eq. Values from the system complexity metric (SCM), technology readiness level (TRL), integration readiness level (IRL), and degree of crew assistance metric identified a simpler, less costly, and easier to operate design for automated biomass dewatering, cell lysis, and protein affinity purification.

show abstract

Gene Expression Measurement Module (GEMM) for space application: Design and validation

Cited by 7 publications

References 118 publications

Theoretical design of a space bioprocessing system to produce recombinant proteins

Theoretical design of a space bioprocessing system to produce recombinant proteins

A Systematic Review of Product Design for Space Instrument Innovation, Reliability, and Manufacturing

Theoretical design of a space bioprocessing system to produce recombinant proteins

Contact Info

Product

Resources

About