Gene expression of cytokinesis regulators PRC1, KIF14 and CIT has no prognostic role in colorectal and pancreatic cancer

Abstract

Colorectal cancer is one of the most common cancers and pancreatic cancer is among the most fatal and difficult to treat. New prognostic biomarkers are urgently needed to improve the treatment of colorectal and pancreatic cancer. Protein regulating cytokinesis 1 ( PRC1 ), kinesin family member 14 ( KIF14 ) and citron Rho-interacting serine/threonine kinase ( CIT ) serve important roles in cytokinesis, are strongly associated with cancer progression and have prognostic potential. The present study aimed to investigate the prognostic relevance of the PRC1, KIF14 and CIT genes in colorectal and pancreatic cancer. PRC1, KIF14 and CIT transcript expression was assessed by reverse transcription-quantitative PCR in tumors and paired distant unaffected mucosa from 67 patients with colorectal cancer and tumors and paired non-neoplastic control tissues from 48 patients with pancreatic cancer. The extent of transcript dysregulation between tumor and control tissues and between groups of patients divided by main clinical characteristics, namely patients' age and sex, disease stage, localization and grade, was determined. Finally, the associations of transcript levels in tumors with disease-free interval and overall survival time were evaluated. PRC1, KIF14 and CIT transcripts were upregulated in tumors compared with control tissues. PRC1, KIF14 and CIT levels strongly correlated to each other in both colorectal and pancreatic tumor and control tissues after correction for multiple testing. However, no significant associations were found among the transcript levels of PRC1, KIF14 and CIT and disease-free interval or overall survival time. In summary, the present study demonstrated mutual correlation of PRC1, KIF14 and CIT cytokinesis regulators with no clear prognostic value in pancreatic and colorectal cancers. Hence, according to the results of the present study, transcript levels of these genes cannot be clinically exploited as prognostic biomarkers in colorectal or pancreatic cancer patients.

Prognostic Impact and Functional Annotations of KIF11 and KIF14 Expression in Patients with Colorectal Cancer

“…In the multivariate Cox model, accounting for additional covariates, the strength of the association increased and was statistically significant. Thus, the present investigation identified KIF14 expression as an independent favorable prognostic factor, although it was not reflected in the other report demonstrating that KIF14 gene expression did not affect the survival of CRC patients [42]. Contrary to the TCGA cohort used in the present study, the cited research was carried out on a relatively small cohort group of patients with little ethnic diversity, and cut-off values for high and low gene expression were established using different methods, which may explain the divergent results.…”

“…According to our results, and unlike the data published for other examined cancers, KIF14 protein expression was downregulated in CRC tissues compared to the adjacent noncancerous tissues. In turn, KIF14 mRNA expression was upregulated in tumors compared to normal tissues, which is in line with the previous reports on various cancers, including colorectal cancer [ 37 , 38 , 39 , 40 , 41 , 42 ]. A mismatch between mRNA and protein levels has been frequently observed and extensively debated in the literature [ 43 , 44 ].…”

A robust six-gene prognostic signature based on two prognostic subtypes constructed by chromatin regulators is correlated with immunological features and therapeutic response in lung adenocarcinoma

“…CIT (Citron Rho-Interacting Serine/Threonine Kinase) is a gene encoding a serine/threonine-protein kinase that functions in cell division. Several recent studies have reported the potential role of CIT gene in the initiation and progression of some tumors [ 55 – 57 ]. The upregulation of CIT could promote the growth of cancer cells [ 56 , 57 ], and was associated with a poor outcome in some cancers such as bladder cancer and pancreatic ductal adenocarcinomas [ 58 , 59 ].…”

“…CIT (Citron Rho-Interacting Serine/Threonine Kinase) is a gene encoding a serine/threonine-protein kinase that functions in cell division. Several recent studies have reported the potential role of CIT gene in the initiation and progression of some tumors [55][56][57].…”

Survey on Mental Health Status and Quality of Life and Correlation among Patients with Permanent Stoma of Colorectal Tumor

“…Although this ratio was lower than that in Western European and American countries, the total number of colorectal cancer cases is still considerable due to the large population base in China [ 10 – 12 ]. In recent years, the incidence rate of colorectal cancer has shown a gradual increasing trend with the improvement of the living standard [ 13 ]. Surgery is the cornerstone of various treatments for colorectal cancer, but the surgical trauma is large, and patients suffer great physical and mental pain during perioperative period [ 14 – 16 ].…”