Gene expression of differentiated parent in teratocarcinoma cell hybrids. Repression or reprogramming?

Forejt, Jiřı́; Gregorová, Soňa; Dohnal, Karel; Nosek, J

doi:10.1016/0045-6039(84)90079-4

Cited by 15 publications

(10 citation statements)

References 15 publications

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“…These cells often have a tetraploid or near-tetraploid complement of chromosomes (Rousset et al 1983, Takagi et al 1983, Forejt et al 1984, Takagi 1993, 1997, Mise et al 1996. A similar chromosome composition has been described for hybrid cells obtained by fusion of primordial germ cells with lymphocytes (Tada et al 1997).…”

Section: Segregation Of the Parental Chromosomes In Es And Eg Hybrid mentioning

confidence: 93%

“…Thus, TC-somatic hybrid cells are able to give rise to true carcinomas containing derivatives of all three embryonic germ layers (Andrews and Goodfellow 1980, Atsumi et al 1982, Rousset et al 1983, Takagi 1983 or to form embryoid bodies in suspension culture (Takagi 1993). The presence of embryonic antigens has been also observed in teratocarcinoma intra-and interspecific hybrids (Forejt et al 1984, Serov et al 1990). However, this was true only in the case when the somatic parents in fusion with the TC cells were lymphocytes or thymocytes.…”

Section: Teratocarcinoma-somatic Cell Hybridsmentioning

confidence: 95%

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Embryonic hybrid cells: a powerful tool for studying pluripotency and reprogramming of the differentiated cell chromosomes

Serov

Matveeva

Kuznetsov

et al. 2001

An. Acad. Bras. Ciênc.

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The properties of embryonic hybrid cells obtained by fusion of embryonic stem (ES) or teratocarcinoma (TC) cells with differentiated cells are reviewed. Usually, ES-somatic or TC-somatic hybrids retain pluripotent capacity at high levels quite comparable or nearly identical with those of the pluripotent partner. When cultured in vitro, ES-somatic-and TC-somatic hybrid cell clones, as a rule, lose the chromosomes derived from the somatic partner; however, in some clones the autosomes from the ES cell partner were also eliminated, i.e. the parental chromosomes segregated bilaterally in the ES-somatic cell hybrids. This opens up ways for searching correlation between the pluripotent status of the hybrid cells and chromosome segregation patterns and therefore for identifying the particular chromosomes involved in the maintenance of pluripotency. Use of selective medium allows to isolate in vitro the clones of ES-somatic hybrid cells in which "the pluripotent" chromosome can be replaced by "the somatic" counterpart carrying the selectable gene. Unlike the TCsomatic cell hybrids, the ES-somatic hybrids with a near-diploid complement of chromosomes are able to contribute to various tissues of chimeric animals after injection into the blastocoel cavity. Analysis of the chimeric animals showed that the "somatic" chromosome undergoes reprogramming during development. The prospects for the identification of the chromosomes that are involved in the maintenance of pluripotency and its cis-and trans-regulation in the hybrid cell genome are discussed.

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Section: Segregation Of the Parental Chromosomes In Es And Eg Hybrid mentioning

confidence: 93%

Section: Teratocarcinoma-somatic Cell Hybridsmentioning

confidence: 95%

Embryonic hybrid cells: a powerful tool for studying pluripotency and reprogramming of the differentiated cell chromosomes

Serov

Matveeva

Kuznetsov

et al. 2001

An. Acad. Bras. Ciênc.

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“…We next looked at modifications at the Thy-1 gene, which is expressed in thymocytes and repressed in ES and hybrid cells (12). The transcriptionally active thymocyte-derived promoter region was characterized by hyperacetylation of histone H3 4E).…”

Section: Resultsmentioning

confidence: 99%

Histone Code Modifications on Pluripotential Nuclei of Reprogrammed Somatic Cells

Kimura

Tada

Nakatsuji

et al. 2004

Molecular and Cellular Biology

192

119

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Following hybridization with embryonic stem (ES) cells, somatic genomes are epigenetically reprogrammed and acquire pluripotency. This results in the transcription of somatic genome-derived tissue-specific genes upon differentiation. During nuclear reprogramming, it is expected that DNA and chromatin modifications, believed to function in cell-type-specific epigenotype memory, should be significantly modified. Indeed, current evidence indicates that acetylation and methylation of histone H3 and H4 amino termini play a major role in the regulation of gene activity through the modulation of chromatin conformation. Here, we show that the reprogrammed somatic genome of ES hybrid cells becomes hyperacetylated at H3 and H4, while lysine 4 (K4) of H3 becomes globally hyper-di-and -tri-methylated. In the Oct4 promoter region, histones H3 and H4 are acetylated and H3-K4 is highly tri-methylated on both the ES and reprogrammed somatic genomes, which correlates with gene activation and DNA demethylation. However, H3-K4 is also di-and tri-methylated in the promoter regions of Neurofilament-M (Nfm), Nfl, and Thy-1, which are all silent in both ES and hybrid cells. Thus, H3-K4 di-and tri-methylation of reprogrammed somatic genomes is independent of gene activity and represents one of the major events that occurs during somatic genome reprogramming towards a transcriptional activation-permissive state.

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“…The molecular dominance of pluripotent embryonic stem (ES), embryonal carcinoma (EC) or embryonic germ (EG) cells is demonstrated by the repression of tissue-specific genes [5], de novo expression of the transcription factor Oct-4 by the somatic genome, reactivation of the previously inactivated X-chromosome, and specific demethylation of imprinted genes [6,7]. The hybrid cells generated in these studies were able to differentiate into various tissues in chimeras in vivo, confirming their pluripotentiality.…”

Section: Recipient Cytoplasm Suitable For Reprogramming a Foreign Nucmentioning

confidence: 99%

Reprogramming in nuclear transfer

Jouneau¹,

Renard²

2003

Current Opinion in Genetics & Development

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Gene expression of differentiated parent in teratocarcinoma cell hybrids. Repression or reprogramming?

Cited by 15 publications

References 15 publications

Embryonic hybrid cells: a powerful tool for studying pluripotency and reprogramming of the differentiated cell chromosomes

Embryonic hybrid cells: a powerful tool for studying pluripotency and reprogramming of the differentiated cell chromosomes

Histone Code Modifications on Pluripotential Nuclei of Reprogrammed Somatic Cells

Reprogramming in nuclear transfer

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