Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves

Leão, Sydney Correia; Souto, Fernanda Maria Silveira; Costa, Ricardo Vieira da; Rocha, Thaisa de Fátima Almeida; Pacheco, Yolanda Galindo; Rodrigues, Tania Maria de Andrade

doi:10.5935/1678-9741.20120092

Cited by 3 publications

(3 citation statements)

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“…In our study, there is gene expression of endothelin-1 in damaged heart valves in patients that underwent mitral valve replacement, resembling those seen in other samples of the Brazilian population. In this sense, Moura et al [ 1 ] found that 40.7% of mitral valves (fibrosed and stenosed) replaced in patients with RF presented gene expression of ET-1 and, in our previous study [ 22 ] , we observed expression of both endothelin receptors (ETrA and ETrB) in replaced rheumatic mitral valves.…”

Section: Discussionsupporting

confidence: 52%

IL-10 and ET-1 as biomarkers of rheumatic valve disease

Leão¹,

Lima²,

Nascimento³

et al. 2014

Revista Brasileira De Cirurgia Cardiovascular

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Objective: To evaluate the immunological profile and gene expression of endothelin-1 (ET-1) in mitral valves of patients with rheumatic fever originated from a reference service in cardiovascular surgery.Methods: This was a quantitative, observational and cross-sectional study. Thirty-five subjects (divided into four groups) participated in the study, 25 patients with chronic rheumatic heart disease and ten control subjects. The mean age of the sample studied was 34.5 years. Seventeen of them (48.58%) were male and 18 (51.42%) were female. Inflammatory cytokines (TNF-α, IL-4 and IL-10) were measured and ten mitral valves of patients who underwent first valve replacement were collected for determination of gene expression of endothelin-1 by real time PCR.Results: Among the groups studied (patients vs. controls), there was a statistically significant difference in IL-10 levels (P=0.002), and no differences in other cytokines. Expression of endothelin-1 was observed in 70% of samples. Quantitatively, average of ET-1 expression was 62.85±25.63%.Conclusion: Inflammatory cytokine IL-10 participates in the maintenance of chronicity of rheumatic fever in patients who underwent valve replacement and those who are undergoing medical treatment. The expression of endothelin-1 in heart valve lesions in patients undergoing mitral valve replacement confirms its association with inflammatory activity in rheumatic fever. Rev Bras Cir Cardiovasc 2014;29(1):25-30 Descriptors

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Section: Discussionsupporting

confidence: 52%

IL-10 and ET-1 as biomarkers of rheumatic valve disease

Leão¹,

Lima²,

Nascimento³

et al. 2014

Revista Brasileira De Cirurgia Cardiovascular

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“…Por fim, também foram observados fibroblastos, linfócitos e áreas de calcificação distrófica (necrose tecidual) e fibrose em todas as amostras analisadas. 6 Brazilian Journal of Health Review, Curitiba, v. 5, n. 6, p. 22514-22524, nov./dec., 2022 A EM reumática leva a um aumento da pressão no átrio esquerdo, gerando assim também um aumento da contração do AE. No entanto eventualmente pode ocorre um remodelamento do AE, em decorrência de taquicardia e sobrecarga crônica de pressão, que desencadeia diversas alterações histológicas dentre elas hipertrofia, necrose e apoptose vindo então a gerar um possível quadro de fibrilação atrial.…”

Section: Fisiopatologiaunclassified

Estenose Mitral em pacientes com histórico de Febre Reumática

Castro¹,

Inácio²,

Oliveira³

et al. 2022

Braz. J. Hea. Rev.

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INTRODUÇÃO: A febre reumática é uma das principais causas de cardiopatias adquiridas na infância e na adolescência, tendo a cardite reumática como principal complicação. O diagnóstico no Brasil é feito pelos critérios de Jones e o tratamento é individual, geralmente clínico, mas podendo ser cirúrgico. O objetivo foi explorar o diagnóstico e o tratamento da doença em questão. MÉTODOS: O trabalho foi realizado com busca em quatro bases de dados, Medical Literature Analysis and Retrievel System Online (Pubmed/Medline), na Scientific Electronic Library Online (SciELO), Literatura Latino-americana e do Caribe em ciência da Saúde (LILACS) e Biblioteca Virtual em Saúde (BVS), seguida de triagem dos artigos que são relevantes ao assunto em questão, totalizando 24 artigos para a construção desse estudo. DISCUSSÃO: A etiologia da estenose mitral de origem reumática tem início com a faringoamigdalite estreptocócica, infecção causada pelo Streptococcus pyogenes. A faringoamigdalite, se não tratada de forma adequada, pode vir a evoluir para febre reumática, e consequentemente levar ao acometimento dos tecidos cardíacos, gerando a estenose mitral. A patogênese da doença pode ser explicada devido a um mimetismo celular, os anticorpos que deveriam se ligar aos antígenos identificam estruturas no corpo do hospedeiro e deflagram a inflamação que gera a doença. Existe certa dificuldade diagnóstica por escassos dados epidemiológicos. Apesar disso, estudos laboratoriais como ecografia, eletrocardiografia, radiografia de tórax e cateterismo cardíaco são grandes aliados, sendo o ecocardiograma com Doppler o principal para confirmar a investigação. o tratamento é baseado em vários parâmetros clínicos e a terapia vai se concentrar principalmente em três fundamentos: primeiro, a prevenção da recorrência da febre reumática, segundo a melhora dos sintomas e terceiro, a redução de eventos tromboembólicos. CONCLUSÃO: Constata-se que a estenose mitral é a principal complicação da febre reumática em crianças e jovens. Porém, têm-se demonstrado certa dificuldade no rastreio e diagnóstico da doença. Por conseguinte, demonstrou-se que o uso de ecocardiografia como rastreio é aliado durante a investigação.

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“…Rheumatic fever (RF) is a recurring, inflammatory and autoimmune disease as a result of complications of infections by Lancefield group A β-hemolytic Streptococcus pyogenes. RF is rare in developed countries but remains a disease with high prevalence in developing countries, and is a major cause of acquired heart diseases in children and young adults, such as mitral or aortic stenosis [ 3 - 6 ] . It is estimated that more than 125000 people are hospitalized per year in Brazil because of acute RF [ 7 ] .…”

Section: Introductionmentioning

confidence: 99%

Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

Leão¹,

Dashwood²,

Andrade³

et al. 2015

Revista Brasileira De Cirurgia Cardiovascular

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IntroductionRheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.ObjectiveThe aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.MethodsImmunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.ResultsET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.ConclusionThe strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.

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Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves

Cited by 3 publications

References 25 publications

IL-10 and ET-1 as biomarkers of rheumatic valve disease

IL-10 and ET-1 as biomarkers of rheumatic valve disease

Estenose Mitral em pacientes com histórico de Febre Reumática

Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

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