Gene Expression of FADS2 mRNA Linked to Intelligence in Exclusively Breast Milk Fed Preterms

Abstract

Breastfeeding is important for brain development and was shown by many studies to result in higher scores Intelligent Quotient (IQ). However, the exact mechanism whereby Breastfeeding influences cognitive development remains unclear. This study aimed to study the expression of genetic code FADS2 linked to the cognitive function in relation to the mode of feeding. The study comprised 30 preterm infants of 32 to 34 weeks gestation age including 15 exclusively breastfed and 15 fed on Infant Milk Formula (IMF) selected from the Neonatal Intensive Care Unit (NICU) in Talkha Central Hospital in Dakahlia Governorate Egypt. Blood samples were drawn at birth and at 12 weeks of life and FADS2 mRNA gene expression-was measured using real time PCR at birth and at 12 weeks of life. Blood indices were measured at 12 weeks. FADS2 mRNA gene expression demonstrated a significant increase from birth to 3 months, with 109 folds rise in breast fed group compared to five folds rise in the artificially fed group at (p<0.001). Breast milk influences gene expression. The higher FADS2 expression observed in the breastfed group can be used to predict higher IQ scores in the breastfed preterm. The mammary gland could be an organ that stipulates and regulates genetic expression in the newborn.

Telomere Length Measurement by Quantitative Real-time PCR: A Molecular Marker for Human Age Prediction

“…Table 1. The primers used in reference DNA amplifications (Alhusseini et al, 2014) ( In the present study there was a gender-specific difference in relative TL; it was significantly (p<0.001) longer in females than males of the corresponding age among all studied individuals. When the studied individuals were classified in to eight age groups representing the decades of life, the mean relative TL was significantly (p<0.05) longer in females than males, except in age groups (<10 years, 31-40 years and 41-50 years) which was insignificantly (p>0.05) longer in females than males, as shown in Fig.…”

“…Concentration of DNA sample was measured = 50 ug mL −1 ×A260 (Alhusseini et al, 2014) Quantitative Real time PCR:…”

Telomere Length as a Molecular Biomarker for Human Age Prediction: A Quantitative Real-Time PCR Study Among Sample of Egyptians

“…It should be nm. Concentration of DNA sample was measured= 50 ug/mL -1 *A260* (Alhusseini et al, 2014).…”

“…; 72 o C,30sec. The primers used in these amplifications are described in table (Alhusseini et al, 2014). Mansoura J. Forensic Med.…”

Association between Helicobacter pylori Infection and Telomere Length: Effect of Eradication Therapy

“…were taken at wave lengths of 260 and 280 nm. Concentration of DNA samples were measured = 30 ng /µL at wave lengths 260 and 280 nm[17].…”