Gene expression of MC3T3-E1 osteoblastic cells on titanium and zirconia surface

Abstract

PURPOSEThis study was performed to define attachment and growth behavior of osteoblast-like cells and evaluate the gene expression on zirconia compared to titanium.MATERIALS AND METHODSMC3T3-E1 cells were cultured on (1) titanium and (2) zirconia discs. The tetrazolium-based colorimetric assay (MTT test) was used for examining the attachment of cells. Cellular morphology was examined by scanning electron microscopy (SEM) and alkaline phosphatase (ALP) activity was measured to evaluate the cell differentiation rate. Mann-Whitney test was used to assess the significance level of the differences between the experimental groups. cDNA microarray was used for comparing the 20215 gene expressions on titanium and zirconia.RESULTSFrom the MTT assay, there was no significant difference between titanium and zirconia (P>.05). From the SEM image, after 4 hours of culture, cells on both discs were triangular or elongated in shape with formation of filopodia. After 24 hours of culture, cells on both discs were more flattened and well spread compared to 4 hours of culture. From the ALP activity assay, the optical density of E1 cells on titanium was slightly higher than that of E1 cells on zirconia but there was no significant difference (P>.05). Most of the genes related to cell adhesion showed similar expression level between titanium and zirconia.CONCLUSIONZirconia showed comparable biological responses of osteoblast-like cells to titanium for a short time during cell culture period. Most of the genes related to cell adhesion and signal showed similar expression level between titanium and zirconia.

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

“…When the MTF was modified with hrBMP‐2 (Figure S4, Supporting Information), the cell shape was more elongated if compared with cells cultured 48 h on the bare MTFs (Figure S5, Supporting Information) or hrBMP‐2 and gentamicin (Figure ). This cell shape and higher filopodia means cells were interacting better when hrBMP‐2 was present on the surface and were well attached. This does not mean that cell attachment was not good on the other substrates, on which focal adhesion points and a well‐arranged cytoskeleton were as well present.…”

Zirconia <i>versus</i> titanium in dentistry: A review

“…Zirconia showed comparable biological responses of osteoblast-like cells to titanium for a short time during the cell culture period. Most genes related to cell adhesion and signal showed a similar expression level between titanium and zirconia 102) . No significant 93) This review started with a PubMed search from 1975 to 2009.…”

Osteoblast integration of dental implant materials after challenge by sub-gingival pathogens: a co-culture study in vitro

“…Osteoblasts-like cell adhesion in monoculture has been found to be enhanced on ZrO 2 compared to pure Ti and also the gene expression of integrin β1, ERK1/2 and c-fos was higher on ZrO 2 than on Ti, 44 although other monoculture studies showed no difference in initial cell response to Ti and ZrO 2 . 45,46 Previous work 11 employing an aerobic, peri-operative, co-culture model consisting of human gingival fibroblasts and supra-gingival oral bacteria, pointed out that a soft tissue seal as needed on the neck part of an implant, develops more readily on bacterially contaminated, smooth Ti surfaces (see also Figure 7, right panels). Therewith our previous 11 and present co-culture studies on dental implant materials indicate that different requirements should be set to the materials properties for the neck versus the osseointegratable part of a dental implant.…”