Gene expression of P53 and adipoq as diagnostic markers for colorectal cancer

Abstract

AbstractÖz Purpose: Colorectal cancer is the most frequent cause of death and had high mortality rate in Western world. It is from complex, variable and patient-specific interaction between genetic, epigenetic and environmental factors. In the present study, we aimed to investigate the contribution of gene expression of the P53 and Adipoq, both genes to the risk of colorectal cancer. P53 gene is a tumor suppressor gene, encoded protein of P53 is a transcription factor and its pivotal role in maintaining genomic stability. Adipoq gene codes adiponectin. Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 32 colorectal cancer patients. The mRNA expression level of P53 and Adipoq were measured employing semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results:The mRNA expression level of P53 in colorectal cancer was significantly increased according to normal samples (over-expressed). However, the mRNA expression level of Adipoq in colorectal cancer was significantly reduced according to normal samples (downregulated). Conclusion: In current study, our data suggest those reduced mRNA expression of the Adipoq and increased mRNA expression of P53 might be useful molecular diagnostic markers for colorectal cancer patients. In order to understand the investigation between colorectal cancer and diagnostic biomarker; further analysis is necessary.Amaç: Kolorektal kanser Batı dünyasında çok sık karşılaşılan ve ölüm oranı yüksek bir hastalıktır. Genetik, epigenetik ve çevresel faktörler arasında, hasta spesifik bir interaksiyon vardır, dolayısı ile çok çeşitli ve komplekstir. Bu çalışmada P53 ve Adipoq genlerinin ekspresyon seviyelerinin, kolorektal kanser riski üzerine etkisini incelemeyi amaçladık. P53 geni tümör süpressör bir gen olup, bir transkripsiyon faktörü olan P53 proteinini kodlar, genomik stabilitenin devamlılığında çok önemlidir. Adipoq geni adiponektini kodlar. Gereç ve Yönetim: Otuz iki kolorektal kanserli hastanın, tümör ve normal dokusundan RNA izolasyonu yapıldı. P53 ve Adipoq mRNA ekspresyon seviyeleri semiquantitative reverse transkripsiyon-polimeraz zincir reaksiyonu (RT-PCR) yöntemi ile belirlendi. Bulgular: Kolorektal kanserde, P53 ekspresyon seviyesinin normal dokuya göre önemli oranda artış gösterdiği tesbit edilmiştir (aşırı ekspresyon). Fakat, Adipoq ekspresyon seviyesinin, kolorektal kanserinde normal dokuya oranla önemli derecede azaldığı belirlenmiştir (azalmış ekspresyon). Sonuç: Yaptığımız bu çalışmada elde ettiğimiz veriler, Adipoq gen ekspresyonu seviyesinin azaldığı, P53 geni ekspresyon seviyesinin ise artış gösterdiğine dair olup, bu genlerin kolorektal kanserli hastalarda teşhis markırı olarak kullanılabileceğini önermektedir. Kolorektal kanser ve teşhis biyomarkırları arasındaki ilişkiyi anlamak için, daha fazla çalışma yapılması gerekir.

KRAS and MT-CO1 genes in colorectal cancer: a molecular investigation

“…Furthermore, Maciag, Sithanandam (27) also reported that mutated KRAS in lung cancer resulted in COX-2 peroxidase activation DNA damage that led to intrinsic apoptosis pathway activation. Although there have been contradicting results on the impact of KRAS on apoptosis, loss of apoptosis is the major hallmark of cancer (28,29). We believe that KRAS mutation might be one of the reasons for preventing apoptosis mainly in CRC.…”

Erbil’de meme kanseri hastalarında P53 geni ekspresyonundaki değişikliklerin ilişkisi

“…The work area was cleaned by 70% (v/v) ethanol and filter tips were used in all steps. The cDNA was amplified by semi-quantitative RT-PCR and employed the expression primers 10 (Table 1). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as a housekeeping gene for the normalization of P53 gene expression data 12 .…”

“…The p53 is the most as often as possible mutated gene in human malignancies and more than half of human tumors contain p53 defects, which is guardian of the genome 7,8 . Its most pivotal ordinary capacity is liable to direct cell cycle arrest at the G1 or G2 phase of the cell cycle after specific sorts of DNA harm and to stimulate apoptosis when the impairment is too severe 9,10 . Human P53 gene, comprises of 11 exons, which is codes a protein with 393 amino acids 11 .…”

“…Human P53 gene, comprises of 11 exons, which is codes a protein with 393 amino acids 11 . A few earlier studies have showed gene and protein expression of p53 via reverse transcriptase-PCR and immunohistochemistry techniques in various tumors 10,11 . In the present study we aimed to assess the conceivable relationship between mRNA expression level of P53 and breast tumor by checking semi-quantitative RT-PCR technique, P53 gene alteration to the risk of breast cancer.…”