Gene expression of proteolytic system of Lactobacillus helveticus H9 during milk fermentation

Zhang, Wen Yi; Chen, Yong Fu; Zhao, Wan‐Lei; Kwok, Lai Yu; Zhang, He Ping

doi:10.1007/s13213-014-0902-3

Cited by 11 publications

(4 citation statements)

References 24 publications

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“…Previous research showed that the cell wall-associated proteinase (PrtP) and the Opp transport system are necessary for the growth of the strain in milk because they participate in the first step of casein hydrolysis and in the utilization of generated peptides (Tynkkynen et al, 1993;Juillard et al, 1995a,b). Although proteinase PrtP was not identified in the present study, expression of prtP was found upregulated in the log and stationary phases, and prtM was upregulated in log phase and then downregulated in stationary phase (Zhang et al, 2014). Additionally, PepE, PepO2, and OppC were highly expressed in both log and stationary phases.…”

Section: Proteins Related To the Proteolytic Systemcontrasting

confidence: 61%

Proteome analysis of Lactobacillus helveticus H9 during growth in skim milk

Chen¹,

Zhao²,

Wu³

et al. 2014

Journal of Dairy Science

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Lactobacillus helveticus H9 was isolated from traditionally fermented yak milk in Tibet (China) with the ability to produce the antihypertensive peptides Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) during milk fermentation. To understand the changes in the protein expression of L. helveticus H9, proteome analysis was performed at 3 different growth stages, lag phase (pH 6.1), log phase (pH 5.1), and stationary phase (pH 4.5) using 2-dimensional electrophoresis (2-DE). Further analysis showed that 257 differential protein spots were found and 214 protein spots were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The cellular functions of the differentially expressed proteins were complex. Interestingly, the proteolytic system-related proteins aminopeptidase N (PepN), aminopeptidase E (PepE), endopeptidase O2 (PepO2), and oligopeptide transport system permease protein (OppC) were observed only on the maps of pH 5.1 and pH 4.5, which was consistent with the presence of angiotensin I-converting enzyme (ACE)-inhibitory peptides VPP and IPP during these 2 growth stages (log phase and stationary phase). These results, combined with a previous study of gene expression of the proteolytic system, led us to conclude that the Opp transport system, pepE, and pepO2 are likely related to the production of ACE-inhibitory peptides.

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Section: Proteins Related To the Proteolytic Systemcontrasting

confidence: 61%

Proteome analysis of Lactobacillus helveticus H9 during growth in skim milk

Chen¹,

Zhao²,

Wu³

et al. 2014

Journal of Dairy Science

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“…To aid efficient acquisition of amino acids, LAB usually possess an effective proteolytic enzyme system that generally consists of multiple cell surface-associated proteinases, transport systems, and peptidases. Before any peptides or amino acids can be translocated to the cytoplasm, proteins would first need to be broken down by the bacterial proteinases (Zhang et al, 2015 ). Overall speaking, the expression of individual protein components of the proteolytic enzyme system was not induced except 1 oligopeptide ABC transporter periplasmic protein (LCAZH_0339), which might be necessary for oligopeptide uptake.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Adaptive Amoxicillin Resistance of Lactobacillus casei Zhang by Proteomic Analysis

et al. 2018

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Amoxicillin is one of the most commonly prescribed antibiotics for bacterial infections and gastrointestinal disorders. To investigate the adaptation of Lactobacillus (L.) casei Zhang to amoxicillin stress, an iTRAQ-based comparative proteomic analysis was performed to compare the protein profiles between the parental L. casei Zhang and its amoxicillin-resistant descendent strains. Our results revealed a significant increase in the relative expression of 38 proteins (>2.0-folds, P < 0.05), while the relative expression of 34 proteins significantly decreased (<−2.0-folds, P < 0.05). The amoxicillin-resistant descendent strain exhibited marked alterations in carbohydrate and amino acid metabolism. Moreover, certain components involving in membrane metabolism were activated. The differences in the proteomic profiles between the two strains might explain the enhanced stress resistance of the adapted bacteria.

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“…Unlike the relatively simple lactose catabolic pathways, the LAB proteolytic systems are made up of a diverse array of enzymes ( Chen et al, 2014 ). Compared with other detected koumiss LAB, the dominant species L. helveticus is characterized with a high proteolytic activity ( Zhang et al, 2015 ). Most LAB possess only one cell-envelope proteinase that initiates the milk casein hydrolysis, whereas L. helveticus contains at least two of these enzymes, namely PrtH and PrtH2 ( Zhao et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique

Yao

Hou

et al. 2017

Front. Microbiol.

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Koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. In the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. This approach aimed at detecting the low-abundant bacteria in the koumiss. Briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. Then, three diluted bacterial suspensions were randomly picked for further study. By analyzing 30 diluted koumiss suspensions, a total of 24 bacterial species were identified. In addition to the previously reported koumiss-associated species, such as Lactobacillus (L.) helveticus. Lactococcus lactis. L. buchneri, L. kefiranofaciens, and Acetobacter pasteurianus, we successfully detected three low-abundant taxa in the samples, namely L. otakiensis. Streptococcus macedonicus, and Ruminococcus torques. The functional koumiss metagenomes carried putative genes that relate to lactose metabolism and synthesis of typical flavor compounds. Our study would encourage the use of modern metagenomics to discover novel species of bacteria that could be useful in food industries.

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Gene expression of proteolytic system of Lactobacillus helveticus H9 during milk fermentation

Cited by 11 publications

References 24 publications

Proteome analysis of Lactobacillus helveticus H9 during growth in skim milk

Proteome analysis of Lactobacillus helveticus H9 during growth in skim milk

Characterization of the Adaptive Amoxicillin Resistance of Lactobacillus casei Zhang by Proteomic Analysis

A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique

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