Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease

Neshan, Masoud; Campbell, Arezoo; Malakouti, Seyed Kazem; Zareii, Mahsa; Ahangari, Ghasem

doi:10.1016/j.heliyon.2020.e04716

Cited by 5 publications

(3 citation statements)

References 84 publications

(94 reference statements)

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“…For example, mRNA levels of 5-HT 2A R in PBMCs from patients with major depression have been reported to be significantly upregulated compared with those in healthy subjects, which is consistent with the reported increase in 5-HT 2A R binding sites in postmortem brain tissue (51)(52)(53). In Alzheimer's disease, a significant decrease in the mRNA level of 5-HT 2A R in PBMCs was reported (54). We speculated that changes in mRNA level of 5-HT 2A R in the brain may be reflected in PBMCs of patients with PIU.…”

Section: Discussionsupporting

confidence: 79%

mRNA Levels of MAOA and 5-HT2A Receptor in Patients With Pathological Internet Use: Correlations With Comorbid Symptoms

Qiu

Zhang²,

Dai

et al. 2021

Front. Psychiatry

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Objective: Uncontrolled internet use may lead to the emergence of pathological internet use (PIU). PIU has become a global public health concern that can cause a range of psychotic symptoms, including anxiety, depression, and impulse control disorder. To date, we know very little about the principal biological factors related to PIU. Monoamine oxidase type A (MAOA) and serotonin (5-HT) 5-HT2A receptor (5-HT2AR) play critical roles in the development of behavioural and drug addictions. Thus, the aim of this study was to measure the relative expression of mRNA of MAOA and 5-HT2AR in peripheral blood mononuclear cells (PBMCs) of patients with PIU and to determine the correlations between these biological indicators and the comorbid symptoms of patients with PIU.Methods: In this study, the mRNA of MAOA and 5-HT2AR was detected using real-time PCR in PBMCs of the patients with PIU (n = 24) and healthy controls (HCs, n = 25). The relationship between the mRNA levels of MAOA and 5-HT2AR and clinical symptoms in patients with PIU was further investigated.Results: MAOA mRNA in PBMCs was significantly upregulated in patients with PIU compared with that in HCs. mRNA levels of 5-HT2AR were not found to differ significantly between HCs and patients with PIU. Correlation analyses further revealed a significant positive correlation between the relative expression of MAOA mRNA in PBMCs of patients with PIU and the Young's Internet Addiction Test and Self-Rating Depression Scale scores.Conclusion: The present study revealed upregulated expression of MAOA mRNA in patients with PIU and an association between the expression of MAOA mRNA and clinical symptoms of PIU, suggesting that the neurobiological changes may be similar between PIU and substance addiction. Additionally, this study demonstrated a potential association between comorbid symptoms and mRNA levels of MAOA.

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Section: Discussionsupporting

confidence: 79%

mRNA Levels of MAOA and 5-HT2A Receptor in Patients With Pathological Internet Use: Correlations With Comorbid Symptoms

Qiu

Zhang²,

Dai

et al. 2021

Front. Psychiatry

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“…Serotonin also affects the production and release of cytokines and cell activation/proliferation. Through the 5-HTR2A receptor, serotonin hinders tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) production [18, 19]. Oncogenic or tumor-suppressive effects of these factors depend on the level of their production, the microenvironment, the type of producing cells, the stage of cancer, and the anti-cancer treatments used [20, 21].…”

Section: Discussionmentioning

confidence: 99%

Serotonin Receptors and Acetylcholinesterase Gene Expression Alternations: The Potential Value on Tumor Microenvironment of Gastric Cancer

et al. 2023

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Introduction: Gastric cancer is one of the common causes of cancer-related death in the world. Neurotransmitters have recently been related to the proliferation of cancer cells, but the role of neurotransmitters in the progression of gastric cancer is still unexplored. The cross-talk between the nervous system and immune cells through serotonin and its receptors in the tumor microenvironment can impact tumor progress. Our purpose is to expose probable changes in serotonin receptors, acetylcholinesterase, and monoamine oxidase A gene expression in gastric cancer. Methods: Transcript of serotonin receptors (5-HTR2A, 5-HTR2B, 5-HTR3A, 5-HTR7) and monoamine oxidase A genes in the peripheral blood mononuclear cells (40 patients and 40 control) and tissue (21 tumors and 21 normal adjacent tissues) were assessed. The gene expression was analyzed by quantitative real-time PCR using suitable primers. Statistical analysis was performed using appropriate software (REST, Prism). Results: Significantly higher amounts of 5-HTR2A, 5-HTR2B, 5-HTR3A, 5-HTR7, and acetylcholinesterase gene transcripts were found in the peripheral blood of gastric cancer patients compared with healthy individuals. The expression of 5-HTR2B and 5-HTR3A genes was significantly higher (p = 0.0250, p = 0.0005, respectively) and the acetylcholinesterase gene was lower in the tissue of patients (p = 0.0119) compared with adjacent normal tissue. Conclusion: This study highlights the role of serotonin receptors in gastric cancer that might have suggestions for the development of novel therapeutics and defensive approaches that target factors associated with the link between the nervous system, cancer cells, and the tumor microenvironment.

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“…As we know, 5-Hydroxytryptamine (5-HT) is primarily involved in the regulation of learning and memory. HTR3A (CS=114.1) was found to be the highest ranked, which has been found that relative mRNA expression of HTR2A, HTR3A (CS=114.1) and MAOA (CS=59.6) were significantly lower in PBMCs of patients with Late-Onset AD (LOAD) compared with controls [66] . At present, 5-HT receptor antagonists as a symptomatic treatment for cognitive and/or behavioral symptoms of AD have been studied [67,68] .…”

Section: Gene Symbolmentioning

confidence: 98%

Research on Potential Mechanism of Shenwu Capsule on Treating Alzheimer's Disease Based on Network Pharmacology

Xing¹,

Chen²,

Wang³

et al. 2023

IJPS

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The etiology and pathogenesis of Alzheimer's disease are extremely complicated and there is no effective cure available currently. Previous studies have proved the effectiveness of Shenwu capsule in treatment of Alzheimer's disease. However, its mechanism of action has not been systematically elucidated. Here, network pharmacology approach was used to revealing the underlying mechanism of Shenwu capsule in the treatment of Alzheimer's disease. The compound database of Shenwu capsule was constructed, and their potential targets were obtained. Alzheimer's disease gene set was acquired from databases. Subsequently, the potential therapeutic targets of Shenwu capsule on Alzheimer's disease by overlapping analysis, which validated by disease ontology and tissue enrichment analysis. Biological process and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed to find the core biological processes and signaling pathways regulated by Shenwu capsule in treating Alzheimer's disease. By constructing and analyzing the protein-protein interaction network and compound-target network, the core targets and key components were identified. A total of 798 compounds and 1105 potential Shenwu capsule targets, 2566 Alzheimer's disease-related genes and 453 Shenwu capsule therapeutic targets were identified. 3102 biological processes were significantly enriched, such as membrane potential, oxidative stress, synaptic transmission, and neuronal death. 194 pathways of significance were identified, including neuroactive ligand-receptor interactions, lipid-atherosclerosis, Alzheimer's disease, cyclic adenosine 3′,5′-monophosphate signaling and calcium signaling. 35 core therapeutic targets and 55 key components were screened out. Network pharmacology provides an effective way to elucidate the mechanism of action and identify the core targets and key compounds of traditional Chinese medicine. Further studies are needed to validate the validity of this prediction.

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Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease

Cited by 5 publications

References 84 publications

mRNA Levels of MAOA and 5-HT2A Receptor in Patients With Pathological Internet Use: Correlations With Comorbid Symptoms

mRNA Levels of MAOA and 5-HT2A Receptor in Patients With Pathological Internet Use: Correlations With Comorbid Symptoms

Serotonin Receptors and Acetylcholinesterase Gene Expression Alternations: The Potential Value on Tumor Microenvironment of Gastric Cancer

Research on Potential Mechanism of Shenwu Capsule on Treating Alzheimer's Disease Based on Network Pharmacology

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