Gene expression pattern of glucose transporters in the skeletal muscles of newly hatched chicks

Shimamoto, Saki; Ijiri, Daichi; Kawaguchi, Mana; Nakashima, Kazuki; Ohtsuka, Akira

doi:10.1080/09168451.2016.1162088

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…Indeed, GLUT4 is important in insulin stimulating glucose uptake from blood in mammals . In spite of the fact that chickens are short of a GLUT4 gene, some studies reported that the insulin responsive glucose transport mechanism also exists in broilers. , Several reports revealed that the mRNA expressions of GLUT1, 3, 8, and 12 are detected in muscles and speculated that these isoforms may play a role in the insulin stimulating glucose transport in broiler chicken muscle. − In the present study, CrPyr injection increased GLUT3 and GLUT8 mRNA expression levels in the breast muscle of broiler chickens at hatch. Cr supplementation could increase the GLUT4 expression in human and rat skeletal muscles. , A recent study has demonstrated that CrPyr injection enhanced the muscle Cr concentration of broilers at hatch .…”

Section: Discussionmentioning

confidence: 42%

In Ovo Feeding of Creatine Pyruvate Increases the Glycolysis Pathway, Glucose Transporter Gene Expression, and AMPK Phosphorylation in Breast Muscle of Neonatal Broilers

Zhao

Gong

Gao

et al. 2018

J. Agric. Food Chem.

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This study aims to investigate in ovo feeding (IOF) of creatine pyruvate (CrPyr) on glucose metabolism, hormone concentration, and the 5'-AMP-activated protein kinase (AMPK) pathway in breast muscle of embryos and neonatal broilers. The three treatments were noninjected control, 0.75% NaCl treatment, and 12 mg CrPyr/egg treatment. The solution was injected on the 17.5 day of incubation. At hatch, 120 male broilers from each treatment were chosen for a 7 day feeding trial. Compared with other treatments, CrPyr treated broilers enhanced insulin and thyroxine levels in plasma, adenosine triphosphate (ATP) concentration, hexokinase and pyruvate kinase activities, glucose transporter protein mRNA expressions, as well as protein abundances of phosphor-liver kinase B1 and phosphor-AMPK in breast muscle at hatch. In conclusion, IOF of CrPyr improved the energy status, increased the gene expression of glucose transporter proteins, and facilitated glycolysis in breast muscle, which may be associated with the activated AMPK pathway.

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Section: Discussionmentioning

confidence: 42%

In Ovo Feeding of Creatine Pyruvate Increases the Glycolysis Pathway, Glucose Transporter Gene Expression, and AMPK Phosphorylation in Breast Muscle of Neonatal Broilers

Zhao

Gong

Gao

et al. 2018

J. Agric. Food Chem.

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“…As for mammals, glucose transport in chickens involves several glucose transporters that play a crucial role in cellular glucose uptake and glucose homeostasis. Among them, GLUT1, 3, 8, and 12 are expressed in chicken muscles (Kono et al, 2005;Coudert et al, 2015Coudert et al, , 2018Shimamoto et al, 2016), with the levels of mRNA expressions of GLUT1, 3, and 8 (but not GLUT12) being particularly high during the post-hatching period in the glycolytic Pectoralis major muscle compared to more oxidative muscles (Shimamoto et al, 2016). Moreover, although GLUT4 is the major glucose transporter in mammalian muscles, it is not expressed in chickens and the insulin-sensitive glucose transporter GLUT12 could partially play the role of GLUT4 in this species (Coudert et al, 2015(Coudert et al, , 2018.…”

Section: Discussionmentioning

confidence: 99%

“…To answer the growing demand for poultry meat, breeding companies have focused their efforts on improving live performances (i.e., growth rate and feed efficiency) and breast meat yield (Rauw et al, 1998;Havenstein et al, 2003a,b). The selection of meat-type chicken lines for increased growth and muscle development has been accompanied by significant anatomical, physiological, and metabolic changes.…”

Section: Introductionmentioning

confidence: 99%

Early Growth and Protein-Energy Metabolism in Chicken Lines Divergently Selected on Ultimate pH

et al. 2021

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In chickens, a divergent selection on the Pectoralis major pHu allowed the creation of the pHu+ and pHu− lines, which represent a unique model for studying the biological control of carbohydrate storage in muscle. The present study aimed to describe the early mechanisms involved in the establishment of pHu+ and pHu− phenotypes. At hatching, pHu+ chicks were slightly heavier but exhibited lower plasma glucose and triglyceride and higher uric acid. After 5 days, pHu+ chicks exhibited higher breast meat yield compared to pHu− while their body weight was no different. At both ages, in vivo muscle glycogen content was lower in pHu+ than in pHu− muscles. The lower ability of pHu+ chicks to store carbohydrate in their muscle was associated with the increased expression of SLC2A1 and SLC2A3 genes coding glucose transporters 1 and 3, and of CS and LDHα coding key enzymes of oxidative and glycolytic pathways, respectively. Reduced muscle glycogen content at hatching of the pHu+ was concomitant with higher activation by phosphorylation of S6 kinase 1/ribosomal protein S6 pathway, known to activate protein synthesis in chicken muscle. In conclusion, differences observed in muscle at slaughter age in the pHu+ and pHu− lines are already present at hatching. They are associated with several changes related to both carbohydrate and protein metabolism, which are likely to affect their ability to use eggs or exogenous nutrients for muscle growth or energy storage.

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“…SLC2A3 ( GLUT3 ), was classically defined as a neuronal glucose transporter due to its high level of expression and initial characterization in nervous tissue [36]. However, SLC2A3 is also highly expressed in other tissues with high energy needs, including muscle [37, 38]. The animals in the HIF group had higher expression levels of genes associated with lipid metabolism, indicating that these genes may contribute to adipogenesis and the maintenance of lipid metabolism in HIF animals.…”

Section: Resultsmentioning

confidence: 99%

Prediction of hub genes associated with intramuscular fat content in Nelore cattle

et al. 2019

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Background The aim of this study was to use transcriptome RNA-Seq data from longissimus thoracis muscle of uncastrated Nelore males to identify hub genes based on co-expression network obtained from differentially expressed genes (DEGs) associated with intramuscular fat content. Results A total of 30 transcriptomics datasets (RNA-Seq) obtained from longissimus thoracis muscle were selected based on the phenotypic value of divergent intramuscular fat content: 15 with the highest intramuscular fat content (HIF) and 15 with the lowest intramuscular fat content (LIF). The transcriptomics datasets were aligned with a reference genome and 65 differentially expressed genes (DEGs) were identified, including 21 upregulated and 44 downregulated genes in HIF animals. The normalized count data from DEGs was then used for co-expression network construction. From the co-expression network, four modules were identified. The topological properties of the network were analyzed; those genes engaging in the most interactions (maximal clique centrality method) with other DEGs were predicted to be hub genes ( PDE4D, KLHL30 and IL1RAP ), which consequently may play a role in cellular and/or systemic lipid biology in Nelore cattle. Top modules screened from the gene co-expression network were identify. The two candidate modules had clear associated biological pathways related to fat development, cell adhesion, and muscle differentiation, immune system, among others. The hub genes belonged in top modules and were downregulated in HIF animals. PDE4D and IL1RAP have known effects on lipid metabolism and the immune system through the regulation of cAMP signaling. Given that cAMP is known to play a role in lipid systems, PDE4D and IL1RAP downregulation may contribute to increased levels of intracellular cAMP and thus may have effects on IF content differences in Nelore cattle. KLHL30 may have effects on muscle metabolism. Klhl protein families play a role in protein degradation. However, the downregulation of this gene and its role in lipid metabolism has not yet been clarified. Conclusions The results reported in this study indicate candidate genes and molecular mechanisms involved in IF content difference in Nelore cattle. Electronic supplementary material The online version of this article (10.1186/s12864-019-5904-x) contains supplementary material, which is available to authorized users.

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Gene expression pattern of glucose transporters in the skeletal muscles of newly hatched chicks

Cited by 8 publications

References 27 publications

In Ovo Feeding of Creatine Pyruvate Increases the Glycolysis Pathway, Glucose Transporter Gene Expression, and AMPK Phosphorylation in Breast Muscle of Neonatal Broilers

In Ovo Feeding of Creatine Pyruvate Increases the Glycolysis Pathway, Glucose Transporter Gene Expression, and AMPK Phosphorylation in Breast Muscle of Neonatal Broilers

Early Growth and Protein-Energy Metabolism in Chicken Lines Divergently Selected on Ultimate pH

Prediction of hub genes associated with intramuscular fat content in Nelore cattle

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