Gene expression patterns and pearl formation in the Japanese pearl oyster (Pinctada fucata): A comparison of gene expression patterns between the pearl sac and mantle tissues

Inoue, Nariaki; Ishibashi, Ryo; Ishikawa, Takashi; Atsumi, Takashi; Aoki, Hideo; Komaru, Akira

doi:10.1016/j.aquaculture.2010.06.036

Cited by 33 publications

(23 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of each matrix protein gene is restricted to epithelial cells in specific parts of a mantle tissue (Takeuchi and Endo 2005). Differences have been reported in expression patterns of these genes encoding several shell matrix proteins between mantle and pearlsac epithelial cells, and among pearl-sac epithelial cells forming different types of pearls (Takeuchi and Endo 2005;Wang et al 2009;Inoue et al 2010Inoue et al , 2011aInoue et al , 2011b. Daily oscillation in the gene expression is also reported (Miyazaki et al 2008).…”

Section: -4 Relationships Between Morphological Characteristics Of mentioning

confidence: 90%

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Awaji¹,

Machii²

2011

Aqua-BioSci. Monogr. (ABSM)

View full text Add to dashboard Cite

is the pallial line, which is an impression of the pallial muscles lined in an arc in the middle of a mantle. Several types of epithelial cells are recognized in the outer epithelium of a pearl oyster mantle (Tsujii 1960;Wada 1966). The most abundant type is named as outer epithelial cells. They are regarded to play a major role in shell formation and regeneration, and their functions are a main theme of this article.A shell of pearl oyster Pinctada fucata, characterized by its beautiful luster over the inner surface, consists of two layers: periostracum and ostracum. The periostracum is a thin, pliable proteinaceous sheet covering the outer surface of the shell. Components of the periostracum are synthesized in a periostracum gland located at the bottom of a periostracal groove between an outer and a middle fold of a mantle. The periostracum is thought to serve as a barrier that iso- Fundamental Studies on in vivo and in vitro AbstractOuter epithelial cells, which constitute a monolayer epithelium covering the outer surface of pearl oyster mantle, play principal roles in shell and pearl formation. In pearl culture, a fragment of the mantle prepared from a donor is implanted into the recipient's gonad together with a small inorganic bead. Histological studies using pearl oyster Pinctada fucata have revealed that the outer epithelial cells emigrate from the allograft, proliferate, and form a pearl sac surrounding the bead. Following the pearl-sac formation, the pearl-sac epithelia start to form calcium carbonate crystals, such as nacre, on the bead showing morphological characteristics closely related with the crystal structures.To investigate cellular mechanisms of the pearl formation, organ and cell culture methods for the outer epithelial cells of pearl oyster mantle were developed. In the organ culture, crystal formation, deposition of shell matrix-like structure, and DNA synthesis of the outer epithelial cells were observed. The outer epithelial cells separated from the mantle started DNA synthesis in co-culture with hemocytes that revealed a part of cellto-cell interactions during the pearl-sac formation processes. Substitution of the cultured outer epithelial cells for a mantle allograft in pearl culture was tested by injection of the cultured cells; the results of which implied future possibilities for the application of the cultured outer epithelial cells for pearl production.

show abstract

Section: -4 Relationships Between Morphological Characteristics Of mentioning

confidence: 90%

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Awaji¹,

Machii²

2011

Aqua-BioSci. Monogr. (ABSM)

View full text Add to dashboard Cite

show abstract

“…The mantle is the most active factor in shell formation; the edge of the mantle plays a central role in the formation of the corneous and prismatic layers, whereas the mantle center is responsible for the nacreous layer formation (Takeuchi and Endo, 2006;Inoue et al, 2010). Owing to the narrow prismatic layer and the fact that it is covered by the nacreous layer in H. cumingii, the mantle pallial is often thought to be involved in the formation of the nacreous layer.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a novel acidic matrix protein hic22 from the nacreous layer of the freshwater mussel, Hyriopsis cumingii

Liu

Jin

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Matrix proteins that either weakly acidic or unusually highly acidic have important roles in shell biomineralization. In this study, we have identified and characterized hic22, a weakly acidic matrix protein, from the nacreous layer of Hyriopsis cumingii. Total protein was extracted from the nacre using 5 M EDTA and hic22 was purified using a DEAE-sepharose column. The N-terminal amino acid sequence of hic22 was determined and the complete cDNA encoding hic22 was cloned and sequenced by rapid amplification of cDNA endspolymerase chain reaction. Finally, the localization and distribution of hic22 was determined by in situ hybridization. Our results revealed that 2 X.J. Liu et al. Genetics and Molecular Research 15 (3): gmr.15038656 hic22 encodes a 22-kDa protein composed of 185 amino acids. Tissue expression analysis and in situ hybridization indicated that hic22 is expressed in the dorsal epithelial cells of the mantle pallial; moreover, significant expression levels of hic22 were observed after the early formation of the pearl sac (days 19-77), implying that hic22 may play an important role in biomineralization of the nacreous layer.

show abstract

“…Previous to this study, the expression of only nine putative biomineralisation genes had been identified within the pearl sac of a pearl oyster species, Pinctada fucata (msi31, n16, nacrein, msi60, prismalin-14, aspein, EFCBP, ACCBP and n19). These studies compared expression patterns of these shell matrix proteins showing differences in expression levels within the pearl sac and between the pearl sac and mantle tissue (Inoue et al, 2009(Inoue et al, , 2010Wang et al, 2009). In the present study, we found 19 putative biomineralisation genes similarly expressed in both species examined indicating little divergence in the biomineralisation processes of pearl formation between these two species.…”

Section: Pearl Sac Gene Expressionmentioning

confidence: 99%

“…Previous technology applied to examine the expression of biomineralisation-related genes has predominantly relied upon the examination of genes on an individual basis (e.g. through real time PCR) (Wang et al, 2009;Inoue et al, 2010). Recent developments in high-throughput mRNA sequencing using next-generation sequencing platforms now provide a potential way to simultaneously examine all biomineralisation genes that are being expressed at one time.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of biomineralisation-related genes within the pearl sac: Host and donor oyster contribution

et al. 2012

View full text Add to dashboard Cite

Gene expression patterns and pearl formation in the Japanese pearl oyster (Pinctada fucata): A comparison of gene expression patterns between the pearl sac and mantle tissues

Cited by 33 publications

References 13 publications

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Isolation and characterization of a novel acidic matrix protein hic22 from the nacreous layer of the freshwater mussel, Hyriopsis cumingii

Transcriptome analysis of biomineralisation-related genes within the pearl sac: Host and donor oyster contribution

Contact Info

Product

Resources

About