Gene expression plasticity facilitates different host feeding in Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae)

Sellamuthu, Gothandapani; Naseer, Aisha; Hradecký, Jaromír; Chakraborty, Amrita; Synek, Jiří; Modlinger, Roman; Roy, Amit

doi:10.1016/j.ibmb.2023.104061

Cited by 4 publications

(1 citation statement)

References 172 publications

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“…Each sample had five biological replicates. Differential gene expression analysis (DGE) was conducted by mapping raw reads to the P. tremula reference genome [ 27 ] using the OmicsBox transcriptomics module (ver 1.4.11) following the developer protocol as described thoroughly in our latest publication [ 115 ]. DGE was performed using pairwise differential expression analysis in OmicsBox, which was based on edgeR software package (Bioconductor project) [ 116 ], deploying a negative binomial Generalized Linear Model (GLM) for multi-factorial statistical analysis to identify differentially abundant transcripts, with FDR corrected p -value < 0.05 and fold change ±2 as thresholds for differentially expressed transcripts (DETs).…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Transcriptomic and Metabolomic Analyses Unveiling the Defence Mechanisms of Populus tremula against Sucking and Chewing Insect Herbivores

Pastierovič,

Mogilicherla,

Hradecký

et al. 2024

IJMS

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Plants and insects coevolved as an evolutionarily successful and enduring association. The molecular arms race led to evolutionary novelties regarding unique mechanisms of defence and detoxification in plants and insects. While insects adopt mechanisms to conquer host defence, trees develop well-orchestrated and species-specific defence strategies against insect herbivory. However, current knowledge on the molecular underpinnings of fine-tuned tree defence responses against different herbivore insects is still restricted. In the current study, using a multi-omics approach, we unveiled the defence response of Populus tremula against aphids (Chaitophorus populialbae) and spongy moths (Lymantria dispar) herbivory. Comparative differential gene expression (DGE) analyses revealed that around 272 and 1203 transcripts were differentially regulated in P. tremula after moth and aphid herbivory compared to uninfested controls. Interestingly, 5716 transcripts were differentially regulated in P. tremula between aphids and moth infestation. Further investigation showed that defence-related stress hormones and their lipid precursors, transcription factors, and signalling molecules were over-expressed, whereas the growth-related counterparts were suppressed in P. tremula after aphid and moth herbivory. Metabolomics analysis documented that around 37% of all significantly abundant metabolites were associated with biochemical pathways related to tree growth and defence. However, the metabolic profiles of aphid and moth-fed trees were quite distinct, indicating species-specific response optimization. After identifying the suitable reference genes in P. tremula, the omics data were further validated using RT-qPCR. Nevertheless, our findings documented species-specific fine-tuning of the defence response of P. tremula, showing conservation on resource allocation for defence overgrowth under aphid and moth herbivory. Such findings can be exploited to enhance our current understanding of molecular orchestration of tree responses against herbivory and aid in developing insect pest resistance P. tremula varieties.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Transcriptomic and Metabolomic Analyses Unveiling the Defence Mechanisms of Populus tremula against Sucking and Chewing Insect Herbivores

Pastierovič,

Mogilicherla,

Hradecký

et al. 2024

IJMS

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Diet breadth in two polyphagousSpodopteramoths in a wide range of host and non-host plants and the potential for range expansion

Roy,

Wäschke,

Chattington

et al. 2024

Preprint

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Several polyphagous moths are severe crop pests. Diet breadth patterns and mechanisms among polyphagous insects provide an excellent system to study ecological and evolutionary processes in herbivores, driving dietary specialization. However, studies of diet breadth on more than a handful of crops are scarce. Here, we estimated the diet breadth in two species of lepidopteran herbivores from the genus Spodoptera: S. littoralis (SL), with host range including both mono- and dicotyledonous plants and S. frugiperda (SF) Corn strain, primarily adapted to different grass species. Larval performance on 23 crop and wild plant species from 17 families from terrestrial and wetland habitats was compared to an artificial diet in no-choice feeding bioassays. SL survived and performed better on most tested plants, particularly on the family level, except on two monocot plants (maize and leek), where SF performed well. There were five wild non-host plants where both generalists failed to survive. Nutrition indices assay corroborates the findings on a subset of plants. In a subset of plants, larval feeding preference correlated partly, and larval attraction correlated well with larval performance. Female oviposition choice showed a weak correlation with larval performance. This weak correlation implies that these traits are decoupled, and other factors are crucial for female host plant selection. During larval dispersal greenhouse experiments, SL and SF larvae strongly tended to migrate onto their suitable host plants, indicating that this is one factor that modulates female host plant selection. In summary, SL has a broader diet breath compared to SF, surviving on wild plants with no previous exposure. The present study provides the first comprehensive data on the diet breadth of two range-expanding and highly invasive polyphagous herbivores.

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Plasticity for the win: Flexible transcriptional response to host plant switches in the comma butterfly (Polygonia c‐album)

Schneider,

Steward,

Celorio‐Mancera

et al. 2024

Molecular Ecology

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Generalist plant‐feeding insects are characterised by a broad host repertoire that can comprise several families or even different orders of plants. The genetic and physiological mechanisms underlying the use of such a wide host range are still not fully understood. Earlier studies indicate that the consumption of different host plants is associated with host‐specific gene expression profiles. It remained, however, unclear if and how larvae can alter these profiles in the case of a changing host environment. Using the polyphagous comma butterfly (Polygonia c‐album) we show that larvae can adjust their transcriptional profiles in response to a new host plant. The switch to some of the host plants, however, resulted in a larger transcriptional response and, thus, seems to be more challenging. At a physiological level, no correspondence for these patterns could be found in larval performance. This suggests that a high transcriptional but also phenotypic flexibility are essential for the use of a broad and diverse host range. We furthermore propose that host switch tests in the laboratory followed by transcriptomic investigations can be a valuable tool to examine not only plasticity in host use but also subtle and/or transient trade‐offs in the evolution of host plant repertoires.

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Gene expression plasticity facilitates different host feeding in Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae)

Cited by 4 publications

References 172 publications

Genome-Wide Transcriptomic and Metabolomic Analyses Unveiling the Defence Mechanisms of Populus tremula against Sucking and Chewing Insect Herbivores

Genome-Wide Transcriptomic and Metabolomic Analyses Unveiling the Defence Mechanisms of Populus tremula against Sucking and Chewing Insect Herbivores

Diet breadth in two polyphagousSpodopteramoths in a wide range of host and non-host plants and the potential for range expansion

Plasticity for the win: Flexible transcriptional response to host plant switches in the comma butterfly (Polygonia c‐album)

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