Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field

Abstract

In a previous investigation we reported that exposure to a moderate (300 mT) static magnetic field (SMF) causes transient DNA damage and promotes mitochondrial biogenesis in human umbilical vein endothelial cells (HUVECs). To better understand the response of HUVECs to the 300 mT SMF, a high-quality subtracted cDNA library representative of genes induced in cells after 4 h of static magnetic exposure was constructed. The global gene expression profile showed that several genes were induced after the SMF exposure. The characterized clones are involved in cell metabolism, energy, cell growth/division, transcription, protein synthesis, destination and storage, membrane injury, DNA damage/repair, and oxidative stress response. Quantitative real-time polymerase chain reaction (qRT-PCR) experiments were performed at 4 and 24 h on four selected genes. Their expression profiles suggest that HUVEC's response to SMF exposure is transient. Furthermore, compared to control cells, an up-regulation of several genes involved in cell growth and division was observed. This up-regulation is likely to be the cause of the slight, but significant, increase in cell proliferation at 12 h post-treatment. These results provide additional support to the notion that SMFs may be harmless to human health, and could support the rationale for their possible use in medical treatments.

Low‐Frequency Magnetic Field of Appropriate Strengths Changed Secondary Metabolite Production and Na+ Concentration of Intracellular and Extracellular Monascus purpureus

“…Similar results have been reported by Wan et al [2017]. There also were reports that the magnetic field induced the relative expression levels of genes involved in cell growth, metabolism, oxidative stress response, and protein synthesis [Cambpbell‐Beachler et al, 1998; Polidori et al, 2012]. Meanwhile, no obvious difference in the content of secondary metabolites was detected at 0.9 or 2.0 mT LF‐MF compared with the control.…”

Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low‐­intensity electromagnetic field

“…Some cell cycle genes were upregulated by the LI‐EMF treatment, which probably contributed to the increased biomass. Polidori et al [] also reported that several genes involved in cell growth and division were upregulated by SMF (300 mT) in cultured human umbilical vein endothelial cells.…”

“…Gravitational and MF variations are synergized to cause global transcriptional changes of Arabidopsis in in vitro callus cultures, mainly on structural, abiotic stress genes, and secondary metabolism genes [Manzano et al, ]. The gene expression profile of cultured human umbilical vein endothelial cells induced by a 300 mT static magnetic field (SMF) indicates that the cells are involved in cell metabolism, energy, cell growth/division, transcription, protein synthesis, destination and storage, membrane injury, DNA damage/repair, and oxidative stress response [Polidori et al, ]. Gao et al [] reported that a strong SMF (14.1 T) changed the expression of 65 genes involved in processes ranging from amino acid synthesis and DNA and energy metabolism to transcription and regulatory functions.…”

Involvement of Na+/K+pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field

“…pump is regulated by protein phosphatases (PPases) and protein kinases A and G (PKA and PKG) (Therien and Blostein 2000;Ramnanan and Storey 2006). SMF-induced changes from literature are up-and downregulation of genes involved in cellular metabolism (Fos, Rbx1, BioB genes), membrane injury (Plscr 1 gene) (Wang et al 2009;Polidori et al 2012); reduction of mitochondrial DNA content and increase of reactive oxygen species (ROS) (Potenza et al 2010); activation of ERK signaling cascade (Prina-Mello et al 2006); inactivation of voltage-gated potassium and calcium channels (VGPC, VGCC, respectively) (Jie-Fei et al 2007;Rosen 1996); induction of an increase and decrease in [Ca 2? ] i ions (Prina-Mello et al 2006;Wang et al 2010) to the lower effective dose at the target tissue used in a number of randomized control trials of the therapeutic applications of magnetic fields for pain management, whereas a strength field of 100 mT was used in promising cell culture studies for Parkinson's disease treatment (Harlow et al 2004;Wolsko et al 2004;Wang et al 2010).…”