Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage

Nozaki, Tadashige; Ohura, Kiyoshi

doi:10.1254/jphs.10163fp

Cited by 12 publications

(9 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a distinction has emerged between DPSC from murine molar and incisor teeth. While they both possess osteo-dentin and adipocyte differentiation potential, erupted murine molars, but not incisors, have been found to have chondrocytic potential [11-13,15]. Janebodin et al .…”

Section: Introductionmentioning

confidence: 99%

Neurogenic potential of dental pulp stem cells isolated from murine incisors

et al. 2014

View full text Add to dashboard Cite

IntroductionInterest in the use of dental pulp stem cells (DPSC) to enhance neurological recovery following stroke and traumatic injury is increasing following successful pre-clinical studies. A murine model of autologous neural stem cell transplantation would be useful for further pre-clinical investigation of the underlying mechanisms. However, while human-derived DPSC have been well characterised, the neurogenic potential of murine DPSC (mDPSC) has been largely neglected. In this study we demonstrate neuronal differentiation of DPSC from murine incisors in vitro.MethodsmDPSC were cultured under neuroinductive conditions and assessed for neuronal and glial markers and electrophysiological functional maturation.ResultsmDPSC developed a neuronal morphology and high expression of neural markers nestin, ßIII-tubulin and GFAP. Neurofilament M and S100 were found in lower abundance. Differentiated cells also expressed protein markers for cholinergic, GABAergic and glutaminergic neurons, indicating a mixture of central and peripheral nervous system cell types. Intracellular electrophysiological analysis revealed the presence of voltage-gated L-type Ca2+ channels in a majority of cells with neuronal morphology. No voltage-gated Na+ or K+ currents were found and the cultures did not support spontaneous action potentials. Neuronal-like networks expressed the gap junction protein, connexin 43 but this was not associated with dye coupling between adjacent cells after injection of the low-molecular weight tracers Lucifer yellow or Neurobiotin. This indicated that the connexin proteins were not forming traditional gap junction channels.ConclusionsThe data presented support the differentiation of mDPSC into immature neuronal-like networks.

show abstract

Section: Introductionmentioning

confidence: 99%

Neurogenic potential of dental pulp stem cells isolated from murine incisors

et al. 2014

View full text Add to dashboard Cite

show abstract

“…DPCs are easily obtained from extracted teeth, possess high proliferative ability, and can be reprogrammed into induced pluripotent stem (iPS) cells at relatively high rates [18], [19]. OCT4, NANOG and SOX2 have been detected in the early passages of cells derived from the dental pulp and might be markers of differentiation of DPCs [20], [21]. p300 can be detected during murine tooth development [22].…”

Section: Introductionmentioning

confidence: 99%

The Histone Acetyltransferase p300 Regulates the Expression of Pluripotency Factors and Odontogenic Differentiation of Human Dental Pulp Cells

et al. 2014

View full text Add to dashboard Cite

p300 is a well-known histone acetyltransferase (HAT) and coactivator that plays vital roles in many physiological processes. Despite extensive research on the involvement of p300 in the regulation of transcription in numerous cell lines, the roles of this protein in regulating pluripotency genes and odontogenic differentiation in human dental pulp cells (HDPCs) are poorly understood. To address this issue, we investigated the expression of OCT4, NANOG and SOX2 and the proliferation and odontogenic differentiation capacity of HDPCs following p300 overexpression. We found that p300 overexpression did not overtly affect the ability of HDPCs to proliferate. The overexpression of p300 upregulated the promoter activity and the mRNA and protein expression of NANOG and SOX2. The HAT activity of p300 appeared to partially mediate the regulation of these factors; indeed, when a mutant form of p300 lacking the HAT domain was overexpressed, the promoter activity and expression of NANOG and SOX2 decreased relative to p300 overexpression but was greater than in the control. Furthermore, we demonstrated that the mRNA levels of the odontogenic marker genes dentine matrix protein-1 (DMP-1), dentin sialophosphoprotein (DSPP), dentin sialoprotein (DSP), osteopontin (OPN) and osteocalcin (OCN) were significantly decreased in HDPCs overexpressing p300 cultured under normal culture conditions and increased in HDPCs inducted to undergo odontogenic differentiation. This finding was further confirmed by measuring levels of alkaline phosphatase (ALP) activity and assessing the formation of mineralized nodules. The HAT activity of p300 had no significant effect on odontogenic differentiation. p300 was recruited to the promoter regions of OCN and DSPP and might be acting as a coactivator to increase the acetylation of lysine 9 of histone H3 of OCN and DSPP. Collectively, our results show that p300 plays an important role in regulating the expression of key pluripotency genes in HDPCs and modifying odontogenic differentiation.

show abstract

“…Dental pulp-derived cells can differentiate into osteogenic, neurogenic, myogenic, and adipogenic lineage cells by applying in vitro induction conditions similar to those used for human bone marrow cells [1][2][3][4][5] . Notably, demethylation by 5-azacytidine triggers myogenic diff erentiation, suggesting that epigenetic modifi cation is involved in lineage diff erentiation in dental pulp cells 5) .…”

Section: Introductionmentioning

confidence: 99%

“…Notably, demethylation by 5-azacytidine triggers myogenic diff erentiation, suggesting that epigenetic modifi cation is involved in lineage diff erentiation in dental pulp cells 5) . Several pluripotent stem cell markers have been identifi ed, including Nanog, Sox2, and Oct3/4 (also known as Pou5f1) 6,7) .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of <i>miR-183</i> Induces Insulin in Dental Pulp Cells

Nozaki

Ohura

2017

J. Hard Tissue Biology.

Self Cite

View full text Add to dashboard Cite

We previously reported that miR-183 was downregulated during the direct conversion of dental pulp cells to insulin-producing cells. To further elucidate the role of miRNA regulation in dental pulp cells during this process, we examined the eff ect of miR-183 inhibition. Insulin expression was detected in induced cells 72 hours after ectopic expression of miR-183 inhibitor. The expression levels of insulin 1 and insulin 2 mRNA increased 1.7-fold and 1.6-fold, respectively. Insulin copy number estimates, following miR-183 inhibition, showed more than 10 copies each of insulin 1 and insulin 2. These data suggest that miR-183 downregulation induces the direct conversion of dental pulp cells to insulinproducing cells.

show abstract

Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage

Cited by 12 publications

References 38 publications

Neurogenic potential of dental pulp stem cells isolated from murine incisors

Neurogenic potential of dental pulp stem cells isolated from murine incisors

The Histone Acetyltransferase p300 Regulates the Expression of Pluripotency Factors and Odontogenic Differentiation of Human Dental Pulp Cells

Inhibition of <i>miR-183</i> Induces Insulin in Dental Pulp Cells

Contact Info

Product

Resources

About