Gene expression profile of dermal mesenchymal stem cells from patients with psoriasis

Hou, Ruixia; Hu, Yan; Niu, Xuping; Chang, Wenjuan; An, Peng; Wang, C; Yang, Yaru; Yan, Xin; Li, J; Liu, R; Li, X; Zhang, K

doi:10.1111/jdv.12420

Cited by 40 publications

(71 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported in our previously published data (Hou et al, 2014), the cells cultured in this study were adherent, CD105 -, CD34 -, HLA -DR -, and presented a typical fibroblast flat triangular or spindle morphology, and could be induced to differentiate into both osteoblasts and adipocytes.…”

Section: Identification Of Cultured Dermal Mscssupporting

confidence: 48%

“…Although the exact mechanism of MSCs remains to be elucidated, soluble cytokines, such as TGF-b (Groh et al, 2005;Nemeth et al, 2010), IL-10 (Beyth et al, 2005), prostaglandin E2 (Aggarwal and Pittenger, 2005), nitric oxide (NO), and iNOS (Ren et al, 2008), are reportedly involved in these functions. In our previous study, we observed that inflammation and angiogenesisrelated genes, such as CXCL14,, and IGFBP5, were differentially expressed in dermal MSCs of patients with psoriasis (Hou et al, 2014). Therefore, researchers have attempted to identify the role of MSCs in the pathogenesis of psoriasis.…”

Section: Discussionmentioning

confidence: 99%

“…Dermal MSCs were isolated and expanded as described in previous studies (Hou et al, 2014, Chang et al, 2015. Non-adherent cells were removed after 48 h, and half the medium was changed every 3-4 days.…”

Section: Culture and Characterization Of Dermal Mscsmentioning

confidence: 99%

“…We have previously reported that some inflammation-and angiogenesis-related genes, such as IL-1β, CXCL14, VEGFα, GATA-6, insulin-like growth factor-binding protein-5 (IGFBP5), lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), and dual-specificity protein phosphatase 1 (DUSP1), are significantly differentially expressed in dermal MSCs from patients with psoriasis (Liu et al, 2013;Hou et al, 2014;Chang et al, 2015). Psoriatic dermal MSCs present an impaired capacity to inhibit T cell proliferation (Liu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray

Rx¹,

Rf²,

Xc³

et al. 2016

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

Section: Identification Of Cultured Dermal Mscssupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Culture and Characterization Of Dermal Mscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray

Rx¹,

Rf²,

Xc³

et al. 2016

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

“…We previously investigated the biological characteristics and related gene expression patterns of bone marrow-derived MSCs in patients with psoriasis Hou et al, 2014). In addition to reduced proliferation activity and increased spontaneous apoptosis, bone marrow-derived MSCs in psoriasis were found to be associated with high expression of stem cell factor and granulocyte-colony stimulating factor, as well as low expression of inflammatory cytokines such as IL-1, IL-3, IL-18, and TNF-α.…”

Section: Introductionmentioning

confidence: 99%

LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis

Chang¹,

Niu²,

Hou³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation status, and cytokine secretion of MSCs in psoriatic skin lesions, as well as some gene expression abnormalities related to inflammation and angiogenesis. We further verified the gene and protein expressions of inflammation-related lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), and angiogenesis-related hematopoietically expressed homeobox (HHEX) in MSCs derived from the skin lesions of psoriasis patients. The gene expression of LITAF, DUSP1, and HHEX in dermal MSCs was measured at the mRNA level using reverse transcription-polymerase chain reaction and the corresponding protein expression levels were analyzed by western (2015) blotting analysis. The gene and protein expression levels of LITAF, HHEX, and DUSP1 in dermal MSCs were significantly lower in psoriasis patients compared to controls. Amplification and western blotting results were consistent with our previously reported gene chip data. Our results suggest that dermal MSCs in psoriatic skin lesions may be involved in the development, progression, and regulation of localized inflammatory abnormalities by reducing the expression of LITAF, HHEX, and DUSP1, which are related to inflammation and angiogenesis.

show abstract

Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis

Han

Niu

Hou

et al. 2020

Cell Biology International

View full text Add to dashboard Cite

The unusual dilatation of dermal capillaries and angiogenesis played important roles in psoriasis. Some genes and proteins of dermal mesenchymal stem cells (DMSCs) from psoriasis are abnormal and related to the function of endothelial cells (ECs). The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization‐related integrins in psoriasis. Human DMSCs, collected from psoriasis lesions and healthy skin, respectively, were cocultured with human umbilical vein endothelial cells (HUVECs). The expression levels of three integrins, that is, αvβ3, αvβ5, and α5β1 in HUVECs were tested by quantitative real‐time polymerase chain reaction and Western blot analysis. The adhesion and migration of HUVECs were detected by adhesion assay and migration assay. The results showed that in psoriasis group, the expression of αVβ3 and α5β1 of HUVECs markedly increased 2.50‐ and 3.71‐fold in messenger RNA levels, and significantly increased 1.63‐ and 1.92‐fold in protein levels, comparing to healthy control group (all p < .05). But β5 was not significantly different between the two groups (p > .05). In addition, compared with control, psoriatic DMSCs promoted HUVECs adhesion by 1.62‐fold and migration by 2.91‐fold (all p < .05). In conclusion, psoriatic DMSCs impact HUVECs adhesion and migration by upregulating the expression of integrins αVβ3 and α5β1.

show abstract

Gene expression profile of dermal mesenchymal stem cells from patients with psoriasis

Abstract: These results indicate that MSCs from the derma of psoriasis patients might be involved in the early development of psoriasis because of their pro-angiogenic potential as well as the immunoregulatory effect.

Cited by 40 publications

References 39 publications

Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray

Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray

LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis

Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis

Contact Info

Product

Resources

About