2009
DOI: 10.1007/s12275-009-0003-3
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Gene expression profile of Helicobacter pylori in response to growth temperature variation

Abstract: A Helicobacter pylori whole-genome DNA microarray was constructed to study expression profiles of H. pylori in response to a sudden temperature transfer from 37 degrees C to 20 degrees C. The expression level of the genome at each of four time points (15, 30, 60, and 120 min) after temperature downshift was compared with that just before cold treatment. Globally, 10.2 % (n=167) of the total predicted H. pylori genes (n=1636) represented on the microarray were significantly differentially expressed (p<0.05) ove… Show more

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Cited by 5 publications
(4 citation statements)
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“…This suggests that a reduction in energy-requiring processes in C. botulinum ATCC 3502 may represent a survival strategy of bacteria during heat shock stress [26]. …”
Section: Resultsmentioning
confidence: 99%
“…This suggests that a reduction in energy-requiring processes in C. botulinum ATCC 3502 may represent a survival strategy of bacteria during heat shock stress [26]. …”
Section: Resultsmentioning
confidence: 99%
“…Transcriptomic profiling showed good internal consistency within treatment conditions and widespread changes in gene expression with more than one third of all genes differentially expressed. Previous literature showed transcriptomic changes in H. pylori with sudden decreased temperature ( Han et al, 2009 ). Approximately equal proportion of DEGs was up and down-regulated similar to findings seen with decreased temperature.…”
Section: Discussionmentioning
confidence: 99%
“…Here, rpoA was used as housekeeping gene for normalization of expression. Fold changes were calculated according to the ΔΔ C T method [23] and genes with an expression ratio ≥ 2.0 were considered to be up‐regulated and those with an expression ratio ≤ 0.5 were considered to be down‐regulated [24]. The expression of each gene was analyzed using three independent cDNA samples with two technical replicates in each run.…”
Section: Methodsmentioning
confidence: 99%