Gene expression profiles of hepatic cell-type specific marker genes in progression of liver fibrosis

Abstract

Analysis of marker genes for specific hepatic cell types can identify the key aspects of fibrogenesis. Sequential activation of inflammatory cells and the self-supporting properties of HSCs play an important role in development of fibrosis.

Serial changes in expression of functionally clustered genes in progression of liver fibrosis in hepatitis C patients

“…and regulates the cellular redox state, and PPT, which is known as a lysosomal hydrolase of long chain fatty acyl CoA and has a role in maintenance of synaptic function, may be related to mitochondrial damage, as we and others have suggested [3,8,9] . RGN, a calcium-binding protein that plays a pivotal role in maintaining cell homeostasis and function, was also down-regulated.…”

“…Marker genes were selected as representative members of functional categories or molecular networks based on their expression changes in an experimental hepatic fibrosis model [3] . Marker genes with statistically significant changes in expression are listed in Table 1.…”

“…Prevention of HCC and inhibition of fibrosis in the early phase is important, but the detailed hepatic biological changes corresponding to the F stage are unclear. To understand the background of the early stage of fibrosis, we previously identified genes that can be used as markers of biological changes in progression of hepatic fibrosis and diagnosis of fibrotic progression; these data were obtained from DNA microarray data from an experimental DMN (dimethylnitrosamine)-treated rat model of hepatic fibrosis [3] . This work led to marker genes that were arranged in functional categories related to fibrosis, based on genes associated with hepatic cell types such as Kupffer cells, hepatic stellate cells, and hepatocytes.…”

Chronic Administration of Valproic Acid Inhibits Activation of Mouse Hepatic Stellate Cells in Vitro and in Vivo

“…2D). Gene expression levels of several genes known to be regulated during HSC activation in vitro and in vivo 3,23 were analyzed during the same 10-day in vitro culture period using qPCR. The strongest VPA-dependent gene expression changes during HSC activation were observed for Acta2 (␣-SMA), Myh11 (smooth muscle myosin), Lox (lysyl oxidase), and Spp1 (secreted phosphoprotein 1, osteopontin), whereas Gfap and Timp1 were not influenced (Fig.…”

Adverse Outcome Pathway on Protein Alkylation Leading to Liver Fibrosis

“…tissue levels of TNF-a (Vajdova et al, 2004), IL-6 expression, measured by immunoassays or Elisa (offered by various companies), soluble CD163 (Grønbaek et al, 2012;Møller, 2012) or increase in expression of Kupffer cell marker genes such as Lyz, Gzmb, and Il1b (Genome U34A Array, Affymetrix) (Takahara et al, 2006). Boltjes et al, 2014;Kegel et al, 2015;Su et al, 2002.…”