Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton’s Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform

Hiew, Vun Vun; Akhir, Haselamirrah Mohd; Teoh, Peik Lin

doi:10.3390/cimb44090291

Cited by 3 publications

(1 citation statement)

References 51 publications

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“…We employed the qRT-PCR methodology according to previously described protocols [16]. In brief, RNA was extracted from SGC7901 and MGC-803 cells and subjected to cDNA synthesis.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qrt-pcr) A...mentioning

confidence: 99%

REST Promotes Autophagy in Gastric Cancer by Transcriptionally Activating FABP6 to Inhibit the Akt/mTOR Signaling Pathway

Luo,

Yu,

Yuan

et al. 2024

Front. Biosci. (Landmark Ed)

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Background: Gastric cancer (GC) is a leading cause of cancer-associated death worldwide. Its molecular mechanisms, especially concerning autophagy and various signaling pathways, are not fully understood. Fatty Acid Binding Protein 6 (FABP6) and RE1 Silencing Transcription Factor (REST) emerge as potential key players in this context. This study sought to analyze the functional relationship of FABP6 and REST concerning autophagy and their implications on the Akt/mTOR signaling pathway within GC cells. Methods: A comprehensive bioinformatics approach was used to identify key prognostic markers in GC. The effects of FABP6 and REST on autophagy along with Akt/mTOR signaling pathways were analyzed by techniques including Western blotting (WB), flow cytometry, Transwell assay, dual luciferase reporter assay, and others. Results: FABP6 was identified as overexpressed in GC, linked with poor prognosis. FABP6 silencing reduces GC cell proliferation, induces S- and G2-phase arrest, and downregulates cyclins CDK2 and CDK4. It also inhibited GC cell invasion/migration and autophagy, effects that were counteracted by MG132. When combined with PI3K inhibitor LY294002c, FABP6 knockdown showed synergistic anti-proliferative effects, modulating the Akt/mTOR pathway. Besides, the transcription factor REST has been shown to directly regulate FABP6 expression, affecting autophagy and the Akt/mTOR signaling pathway in a FABP6-dependent manner. Conclusions: REST positively regulates autophagy and negatively affects the Akt/mTOR signaling pathway in GC cells in a FABP6-dependent manner, providing valuable insights into regulatory networks involving FABP6 and REST.

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“…We employed the qRT-PCR methodology according to previously described protocols [16]. In brief, RNA was extracted from SGC7901 and MGC-803 cells and subjected to cDNA synthesis.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qrt-pcr) A...mentioning

confidence: 99%

REST Promotes Autophagy in Gastric Cancer by Transcriptionally Activating FABP6 to Inhibit the Akt/mTOR Signaling Pathway

Luo,

Yu,

Yuan

et al. 2024

Front. Biosci. (Landmark Ed)

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Research Progress on the Osteogenesis-Related Regulatory Mechanisms of Human Umbilical Cord Mesenchymal Stem Cells

Jiang

Meng

et al. 2023

Stem Cell Rev and Rep

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Porphyromonas gingivalis LPS‐stimulated BMSC‐derived exosome promotes osteoclastogenesis via miR‐151‐3p/PAFAH1B1

Dong,

Liao,

Zhou

et al. 2024

Oral Diseases

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ObjectivesPorphyromonas gingivalis‐LPS regulated bone metabolism by triggering dysfunction of osteoblasts directly, and affecting activity of osteoclasts through intracellular communication. Exosome, as the mediator of intercellular communication, was important vesicle to regulate osteogenesis and osteoclastogenesis. This research was designed for investigating the mechanism of BMSCs‐EXO in modulating osteoclastic activity under the P. gingivalis‐LPS.Materials and methodsThe cytotoxicity and osteogenic effects of P. gingivalis‐LPS on BMSCs was evaluated, and then osteoclastic activity of RAW264.7 co‐cultured with exosomes was detected. Besides, Affymetrix miRNA array and luciferase reporter assay were used to identify the target exosomal miRNA signal pathway.ResultsBMSCs' osteogenic differentiation and proliferation were decreased under 1 and 10 μg/mL P. gingivalis‐LPS. Osteoclastic‐related genes and proteins levels were promoted by P. gingivalis‐LPS‐stimulated BMSCs‐EXO. Based on the miRNA microarray analysis, exosomal miR‐151‐3p was lessened in BMExo‐LPS group, which facilitated osteoclastic differentiation through miR‐151‐3p/PAFAH1B1.ConclusionsPorphyromonas gingivalis‐LPS could regulated bone metabolism by inhibiting proliferation and osteogenesis of BMSCs directly. Also, P. gingivalis‐LPS‐stimulated BMSCs‐EXO promoted osteoclastogenesis via activating miR‐151‐3p/PAFAH1B1 signal pathway.

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Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton’s Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform

Cited by 3 publications

References 51 publications

REST Promotes Autophagy in Gastric Cancer by Transcriptionally Activating FABP6 to Inhibit the Akt/mTOR Signaling Pathway

REST Promotes Autophagy in Gastric Cancer by Transcriptionally Activating FABP6 to Inhibit the Akt/mTOR Signaling Pathway

Research Progress on the Osteogenesis-Related Regulatory Mechanisms of Human Umbilical Cord Mesenchymal Stem Cells

Porphyromonas gingivalis LPS‐stimulated BMSC‐derived exosome promotes osteoclastogenesis via miR‐151‐3p/PAFAH1B1

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